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Molecular mapping of a new powdery mildew resistance gene Pm2b in Chinese breeding line KM2939

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Abstract

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An allele of Pm2 for wheat powdery mildew resistance was identified in a putative Agropyron cristatum -derived line and used in wheat breeding programs.

Abstract

Powdery mildew (caused by Blumeria graminis f. sp. tritici, Bgt) is one of the most devastating wheat diseases worldwide. It is important to exploit varied sources of resistance from common wheat and its relatives in resistance breeding. KM2939, a Chinese breeding line, exhibits high resistance to powdery mildew at both the seedling and adult stages. It carries a single dominant powdery mildew resistance (Pm) allele of Pm2, designated Pm2b, the previous allelic designation Pm2 will be re-designated as Pm2a. Pm2b was mapped to chromosome arm 5DS and flanked by sequence characterized amplified region (SCAR) markers SCAR112 and SCAR203 with genetic distances of 0.5 and 1.3 cM, respectively. Sequence tagged site (STS) marker Mag6176 and simple sequence repeat (SSR) marker Cfd81 co-segregated with SCAR203. Pm2b differs in specificity from donors of Pm2a, Pm46 and PmLX66 on chromosome arm 5DS. Allelism tests indicated that Pm2b, Pm2a and PmLX66 are allelic. Therefore, Pm2b appears to be a new allele at the Pm2 locus. The closely linked markers were used to accelerate transfer of Pm2b to wheat cultivars in current production.

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Abbreviations

AFLP:

Amplified fragment length polymorphism

Bgt :

Blumeria graminis f. sp. tritici

BSA:

Bulked segregant analysis

GISH:

Genomic in situ hybridization

MAS:

Marker-assisted selection

NIL:

Near-isogenic line

Pm :

Powdery mildew resistance gene

RAPD:

Random amplified polymorphic DNA

RFLP:

Restriction fragment length polymorphism

RGC:

Recipient genome composition

SCAR:

Sequence characterized amplified region

SSR:

Simple sequence repeat

STS:

Sequence tagged site

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Acknowledgments

We are grateful to Dr. R.A. McIntosh, University of Sydney, Australia for critically reviewing drafts of this paper. This research was financially supported by the National High-Tech Research and Development Program of China No. 2011AA1001, the National Natural Science Foundation of China No. 31171550, the National Scientific and Technological Supporting Program of China No. 2013BAD01B02 and the Chinese Academy of Sciences No. XDA08030107.

Conflict of interest

The authors (Pengtao Ma, Hongxing Xu, Yunfeng Xu, Lihui Li, Yanmin Qie, Qiaoling Luo, Xiaotian Zhang, Xiuquan Li, Yilin Zhou and Diaoguo An) declare that our experiments comply with the current laws of China and we have no conflicts of interest.

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Authors and Affiliations

  1. Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050021, China

    Pengtao Ma, Hongxing Xu, Yunfeng Xu, Yanmin Qie, Qiaoling Luo, Xiaotian Zhang & Diaoguo An

  2. The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Lihui Li & Xiuquan Li

  3. The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

    Yilin Zhou

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  1. Pengtao Ma
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  2. Hongxing Xu
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  3. Yunfeng Xu
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  4. Lihui Li
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  5. Yanmin Qie
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Correspondence to Lihui Li or Diaoguo An.

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Ma, P., Xu, H., Xu, Y. et al. Molecular mapping of a new powdery mildew resistance gene Pm2b in Chinese breeding line KM2939. Theor Appl Genet 128, 613–622 (2015). https://doi.org/10.1007/s00122-015-2457-5

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