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Putative Thinopyrum intermedium-derived stripe rust resistance gene Yr50 maps on wheat chromosome arm 4BL

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Abstract

Stripe rust-resistant wheat introgression line CH223 was developed by crossing the resistant partial amphiploid TAI7047 derived from Thinopyrum intermedium with susceptible cultivars. The resistance is effective against all the existing Chinese stripe rust races, including the most widely virulent and predominant pathotypes CYR32 and CYR33. Cytological analyses using GISH detected no chromosomal segments from Th. intermedium. It was presumed that the segment was too small to be detected. Normal bivalent pairing at meiosis in CH223 and its hybrids confirmed its stability. Genetic analysis of the F1, F2, F3 and BC1 populations from crosses of CH223 with susceptible lines indicated that resistance was controlled by a single dominant gene. The resistance gene was mapped using an F2:3 population from Taichung 29/CH223. The gene was linked to five co-dominant genomic SSR markers, Xgwm540, Xbarc1096, Xwmc47, Xwmc310 and Xgpw7272, and flanked by Xbarc1096 and Xwmc47 at 8.0 and 7.2 cM, respectively. Using the Chinese Spring nulli-tetrasomic and ditelosomic lines, the polymorphic markers and the resistance gene were assigned to chromosome arm 4BL. As no permanently named stripe rust resistance genes had been assigned to chromosome 4BL, this new resistance gene is designated Yr50. The gene, together with the identified closely linked markers, could be used in marker-assisted selection to combine two or more resistance genes in a single genotype.

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Acknowledgments

The authors are grateful to Drs. Robert McIntosh and Peng Zhang (Plant Breeding Institute, University of Sydney) and Dr. Xianming Chen (Washington State University) for critical reviews of this manuscript, to Dr. Fangpu Han for technical guidance in GISH analysis and to Prof. Zhensheng Kang (Northwest A&F University, China) for seedling resistance testing. This study was funded by the National Natural Science Foundation (31171839 and 30671299), Shanxi Provincial Program of International S & T Cooperation (2012081006-2) and Research Project Supported by Shanxi Scholarship Council (2012-102) of China and the Opening Project of State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Science.

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Authors and Affiliations

  1. College of Life Science, Shanxi University, Taiyuan, 030006, Shanxi, China

    Jie Liu

  2. Institute of Crop Science, Shanxi Academy of Agricultural Sciences, Taiyuan, 030031, Shanxi, China

    Zhijian Chang, Xiaojun Zhang, Xin Li, Haixian Zhan & Huijuan Guo

  3. Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan, 030031, Shanxi, China

    Zhijian Chang, Xiaojun Zhang, Xin Li, Haixian Zhan & Huijuan Guo

  4. School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan, China

    Zujun Yang

  5. College of Agronomy, Shanxi Agricultural University, Taigu, 030801, Shanxi, China

    Juqing Jia & Jianming Wang

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  1. Jie Liu
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Correspondence to Zhijian Chang.

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Communicated by B. Keller.

J. Liu and Z. Chang contributed equally to this work.

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Liu, J., Chang, Z., Zhang, X. et al. Putative Thinopyrum intermedium-derived stripe rust resistance gene Yr50 maps on wheat chromosome arm 4BL. Theor Appl Genet 126, 265–274 (2013). https://doi.org/10.1007/s00122-012-1979-3

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  • DOI: https://doi.org/10.1007/s00122-012-1979-3

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