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Molecular cytogenetic characterization of a new wheat–rye 4R chromosome translocation line resistant to powdery mildew

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Abstract

Rye is an important and valuable gene resource for wheat improvement. However, due to extensive growing of cultivars with disease resistance genes from short arm of rye chromosome 1R and coevolution of pathogen virulence and host resistance, these cultivars successively lost resistance to pathogens. Identification and deployment of new resistance gene sources in rye are, therefore, of especial importance and urgency. A new wheat–rye line, designated as WR41-1, was produced through distant hybridization and chromosome engineering protocols between common wheat cultivar Xiaoyan 6 and rye cultivar German White. It was proved to be a new wheat–rye T4BL·4RL and T7AS·4RS translocation line using sequential genomic in situ hybridization (GISH), multicolor fluorescence in situ hybridization (mc-FISH), and expressed sequence tag-simple sequence repeat (EST-SSR) marker analysis. WR41-1 showed high levels of resistance to powdery mildew (Blumeria graminis f. sp. tritici, Bgt) pathogens prevalent in China at the adult growth stage and 13 of 23 Bgt isolates tested at the seedling stage. According to its resistant pattern to 23 different Bgt isolates, WR41-1 may possess new gene(s) for resistance to powdery mildew, which differed from previously identified and known powdery mildew genes from rye (Pm7, Pm8, Pm17, and Pm20). In addition, WR41-1 was cytologically stable, had a desirable fertility, and is expected to be useful in wheat improvement.

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Abbreviations

Bgt:

Blumeria graminis f. sp. tritici

CS:

Chinese Spring wheat

DAPI:

4,6-Diamidino-2-phenylindole

EST-SSR:

Expressed sequence tag-simple sequence repeat

FISH:

Fluorescence in situ hybridization

FITC:

Fluorescence isothiocyanate

GISH:

Genomic in situ hybridization

IT:

Infection type

mc-FISH:

Multicolor FISH

PCR:

Polymerase chain reaction

TKW:

Thousand-kernel weight

References

  • An DG, Zhong GC, Li JM, Wang ZG, Wang YM, Ji J (2003) Chromosome doubling methods of immature embryo plants of wheat distant hybridization. Acta Agro Sin 6:955–957

    Google Scholar 

  • An DG, Li LH, Li JM, Li HJ, Zhu YG (2006) Introgression of resistance to powdery mildew conferred by chromosome 2R by crossing wheat nullisomic 2D with rye. J Integr Plant Biol 48:838–847

    Article  Google Scholar 

  • Borner A, Korzun V, Voylokov AV, Worland AJ, Weber WE (2000) Genetic mapping of quantitative trait loci in rye (Secale cereale L.). Euphytica 116:203–209

    Article  CAS  Google Scholar 

  • Catarino S, Alvarez E, Campa A, Vieira R, Roca A, Giraldez R (2006) Identification and physical mapping of induced translocation breakpoints involving chromosome 1R in rye. Chromosome Res 14:755–765

    Article  PubMed  CAS  Google Scholar 

  • Curtis CA, Lukaszewski AJ (1993) Localization of genes in rye that restore male fertility to hexaploid wheat with timopheevi cytoplasm. Plant Breeding 111:106–112

    Article  Google Scholar 

  • Duan XY, Sheng BQ, Zhou YL, Xiang QJ (1998) Monitoring of the virulence population of Erysiphe graminis DC. f. sp. tritici E. Marchal. Acta Phytopathol Sin 25:31–36

    Google Scholar 

  • Endo TR (2007) The gametocidal chromosome as a tool for chromosome manipulation in wheat. Chromosome Res 15:67–75

    Article  PubMed  CAS  Google Scholar 

  • Falke KC, Susic Z, Wilde P et al (2009) Testcross performance of rye introgression lines developed by marker-assisted backcrossing using an Iranian accession as donor. Theor Appl Genet 118:1225–1238

    Article  PubMed  CAS  Google Scholar 

  • Friebe B, Hatchett JH, Gill BS, Sebesta EE (1991) Transfer of hessian fly resistance from rye to wheat via radiation-induced terminal and intercalary chromosomal translocations. Theor Appl Genet 83:33–40

    Article  Google Scholar 

  • Friebe B, Heun M, Tuleen N, Zeller FJ, Gill BS (1994) Cytogenetically monitored transfer of powdery mildew resistance from rye into wheat. Crop Sci 34:621–625

    Article  Google Scholar 

  • Friebe B, Jiang J, Raupp W, McIntosh R, Gill B (1996) Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91:59–87

    Article  Google Scholar 

  • Friebe B, Kynast RG, Gill BS (2000) Gametocidal factor-induced structural rearrangements in rye chromosomes added to common wheat. Chromosome Res 8:501–511

    Article  PubMed  CAS  Google Scholar 

  • Fu SL, Tang ZX, Ren ZL, Zhang HQ (2010) Transfer to wheat (Triticum aestivum) of small chromosome segments from rye (Secale cereale) carrying disease resistance genes. J Applied Genetics 51:115–121

    Article  CAS  Google Scholar 

  • Han FP, Lamb JC, Birchler JA (2006) High frequency of centromere inactivation resulting in stable dicentric chromosomes of maize. P Natl Acad Sci USA 103:3238–3243

    Article  CAS  Google Scholar 

  • Han FP, Gao Z, Birchler JA (2009) Centromere inactivation and reactivation reveals both epigenetic and genetic components for centromere specification. Plant Cell 21:1929–1939

    Article  PubMed  CAS  Google Scholar 

  • He ZH, Rajaram S, Xin ZY, Huang GZ (2001) A history of wheat breeding in China. CIMMYT, Mexico

    Google Scholar 

  • He ZH, Lan CX, Chen XM, Zou YC, Zhuang QS, Xia XC (2011) Progress and perspective in research of adult-plant resistance to stripe rust and powdery mildew in wheat. Sci Agric Sin 44:2193–2215

    Google Scholar 

  • Heun M, Friebe B (1990) Introgression of powdery mildew resistance from rye into wheat. Phytopathology 80:242–245

    Article  Google Scholar 

  • Heun M, Friebe B, Bushuk W (1990) Chromosomal location of the powdery mildew resistance gene of Amigo wheat. Phytopathology 80:1129–1133

    Article  Google Scholar 

  • Hsam SLK, Zeller FJ (1997) Evidence of allelism between genes Pm8 and Pm17 and chromosomal location of powdery mildew and leaf rust resistance genes in the common wheat cultivar ‛Amigo’. Plant Breeding 116:119–122

    Article  Google Scholar 

  • Huang J, Zhao ZH, Song FJ et al (2012) Molecular detection of a gene effective against powdery mildew in the wheat cultivar Liangxing 66. Mol Breeding 30:1737–1745

    Article  CAS  Google Scholar 

  • Hysing SC, Hsam SLK, Singh RP et al (2007) Agronomic performance and multiple disease resistance in T2BS·2RL wheat–rye translocation lines. Crop Sci 47:254–260

    Article  Google Scholar 

  • Jiang JM, Friebe B, Gill BS (1994) Recent advances in alien gene transfer in wheat. Euphytica 73:199–212

    Article  Google Scholar 

  • Koeszegi B, Farshadfar E, Vagujfalvi A, Sutka J (1996) Drought tolerance studies on wheat/rye disomic chromosome addition lines. Acta Agron Hun 44:121–126

    Google Scholar 

  • Korzun V, Malyshev S, Voylokov A, Borner A (1997) RFLP-based mapping of three mutant loci in rye (Secale cereale L.) and their relation to homoeologous loci within the Gramineae. Theor Appl Genet 95:468–473

    Article  CAS  Google Scholar 

  • Lee TG, Hong MJ, Johnson JW, Bland DE, Kim DY, Seo YW (2009) Development and functional assessment of EST-derived 2RL-specific markers for 2BS·2RL translocations. Theor Appl Genet 119:663–673

    Article  PubMed  CAS  Google Scholar 

  • Li HJ, Conner RL, McCallum BD et al (2004) Resistance of Tangmai 4 wheat to powdery mildew, stem rust, leaf rust, and stripe rust and its chromosome composition. Can J Plant Sci 84:1015–1023

    Article  Google Scholar 

  • Li ZS, Li B, Tong YP (2008) The contribution of distant hybridization with decaploid Agropyron elongatum to wheat improvement in China. J Genet Genomics 35:451–456

    Article  PubMed  Google Scholar 

  • Lind V (1982) Analysis of the resistance of wheat–rye addition lines to powdery mildew of wheat (Erysiphe graminis f. sp. tritici). Tagungsber Akad Landwirtsch Wiss DDR 198:509–520

    Google Scholar 

  • Masoudi-Nejad A, Nasuda S, McIntosh RA, Endo TR (2002) Transfer of rye chromosome segments to wheat by a gametocidal system. Chromosome Res 10:349–357

    Article  PubMed  CAS  Google Scholar 

  • McDonald BA, Linde C (2002) The population genetics of plant pathogens and breeding strategies for durable resistance. Euphytica 124:163–180

    Article  CAS  Google Scholar 

  • McIntosh RA, Yamazaki Y, Dubcovsky J et al. A catalogue of gene symbols for wheat. In: Proceedings of the 11th international wheat genetics symposium, Brisbane, Qld Australia, August 24–29 2008.

  • McIntosh RA, Zhang P, Cowger C, Parks R, Lagudah ES, Hoxha S (2011) Rye-derived powdery mildew resistance gene Pm8 in wheat is suppressed by the Pm3 locus. Theor Appl Genet 123:359–367

    Article  PubMed  CAS  Google Scholar 

  • Mcintyre CL, Pereira S, Moran LB, Appels R (1990) New Secale cereale (rye) DNA derivatives for the detection of rye chromosome segments in wheat. Genome 33:635–640

    Article  PubMed  CAS  Google Scholar 

  • Miftahudin, Scoles GJ, Gustafson JP (2002) AFLP markers tightly linked to the aluminum-tolerance gene Alt3 in rye (Secale cereale L.). Theor Appl Genet 104:626–631

  • Mukai Y, Nakahara Y, Yamamoto M (1993) Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes. Genome 36:489–494

    Article  PubMed  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Pedersen C, Langridge P (1997) Identification of the entire chromosome complement of bread wheat by two-colour FISH. Genome 40:589–593

    Article  PubMed  CAS  Google Scholar 

  • Rabinovich SV (1998) Importance of wheat–rye translocations for breeding modern cultivars of Triticum aestivum L. Euphytica 100:323–340

    Article  Google Scholar 

  • Rayburn AL, Gill BS (1986) Isolation of a D-genome specific repeated DNA sequence from Aegilops squarrosa. Plant Mol Biol Rep 4:102–109

    Article  CAS  Google Scholar 

  • Ren TH, Yang ZJ, Yan BJ, Zhang HQ, Fu SL, Ren ZL (2009) Development and characterization of a new 1BL·1RS translocation line with resistance to stripe rust and powdery mildew of wheat. Euphytica 169:207–213

    Article  Google Scholar 

  • Sharp PJ, Kreis M, Shewry PR, Gale MD (1988) Location of β-amylase sequences in wheat and its relatives. Theor Appl Genet 75:286–290

    Article  CAS  Google Scholar 

  • Sheng BQ, Duan XY (1991) Improvement of scale 0–9 method for scoring adult plant resistance to powdery mildew of wheat. Beijing Agricultural Sciences 1:38–39

    Google Scholar 

  • Si QM, Zhang XX, Duan XY, Sheng BQ, Zhou YL (1992) On gene analysis and classification of powdery mildew (Erysiphe graminis f. sp. tritici) resistant wheat varieties. Acta Phytopathol Sin 22:349–355

    Google Scholar 

  • Sidhu MC, Satija CK, Sharma I (2001) Screening of wheat–rye addition lines for karnal bunt resistance. Crop Improv 28:214–217

    Google Scholar 

  • Tang ZX, Fu SL, Ren ZL et al (2008) Production of a new wheat cultivar with a different 1B·1R translocation with resistance to powdery mildew and stripe rust. Cereal Res Commun 36:451–460

    Article  CAS  Google Scholar 

  • Tixier MH, Sourdille PMS (1997) Detection of wheat microsallite using no-radioactive silver nitrate staining method. Genet Breed 51:175–177

    CAS  Google Scholar 

  • Wang CM, Li LH, Zhang XT, Gao Q, Wang RF, An DG (2009a) Development and application of EST-STS markers specific to chromosome 1RS of Secale cereale. Cereal Res Commun 37:13–21

    Article  CAS  Google Scholar 

  • Wang CM, Zheng Q, Li LH et al (2009b) Molecular cytogenetic characterization of a new T2BL·1RS wheat–rye chromosome tanslocation line resistant to stripe rust and powdery mildew. Plant Dis 93:124–129

    Article  CAS  Google Scholar 

  • Wang D, Zhuang LF, Sun L, Feng YG, Pei ZY, Qi ZJ (2010) Allocation of a powdery mildew resistance locus to the chromosome arm 6RL of Secale cereale L. cv. 'Jingzhouheimai'. Euphytica 176:157–166

    Article  CAS  Google Scholar 

  • Xu HX, Yin DD, Li LH et al (2012) Development and application of EST-based markers specific for chromosome arms of rye (Secale cereale L.). Cytogenet Genome Res 136:220–228

    Article  PubMed  CAS  Google Scholar 

  • Zheng Q, Li B, Zhang XY, Mu SM, Zhou HP, Li ZS (2006) Molecular cytogenetic characterization of wheat–Thinopyrum ponticum translocations bearing blue-grained gene(s) induced by r-ray. Euphytica 152:51–60

    Article  Google Scholar 

  • Zhou YL, Duan XY, Chen G, Sheng BQ, Zhang Y (2002) Analyses of resistance genes of 40 wheat cultivars or lines to wheat powdery mildew. Acta Phytopathol Sin 32:301–305

    Google Scholar 

  • Zhou Y, He ZH, Zhang GS et al (2004) Utilization of 1BL·1RS translocation in wheat breeding in China. Acta Agron Sin 30:531–535

    CAS  Google Scholar 

  • Zhuang Q (2003) Chinese wheat improvement and pedigree analysis. Chinese Agriculture Press, Beijing

    Google Scholar 

  • Zhuang Q, Li Z (1993) Present status of wheat breeding and related genetic study in China. Wheat Information Service:1–15

  • Zhuang LF, Sun L, Li AX, Chen TT, Qi ZJ (2011) Identification and development of diagnostic markers for a powdery mildew resistance gene on chromosome 2R of Chinese rye cultivar Jingzhouheimai. Mol Breeding 27:455–465

    Article  CAS  Google Scholar 

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Acknowledgments

This research was supported by the National High-Tech Research and Development Program of China No. 2011AA1001, the National Natural Science Foundation of China No. 31171550, and the National Scientific and Technological Supporting Program of China No. 2013BAD01B02.

Conflict of interest

The authors (Diaoguo An, Qi Zheng, Yilin Zhou, Pengtao Ma, Zhenling Lv, Lihui Li, Bin Li, Qiaoling Luo, Hongxing Xu and Yunfeng Xu) declare that our experiments comply with the current laws of China and we have no conflict of interest.

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Authors and Affiliations

  1. Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology (IGDB), Chinese Academy of Sciences (CAS), Shijiazhuang, 050021, China

    Diaoguo An, Pengtao Ma, Qiaoling Luo, Hongxing Xu & Yunfeng Xu

  2. The State Key Laboratory of Plant Cell and Chromosome Engineering, IGDB, CAS, Beijing, 100101, China

    Qi Zheng, Zhenling Lv & Bin Li

  3. The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100081, China

    Lihui Li

  4. The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, CAAS, Beijing, 100193, China

    Yilin Zhou

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  1. Diaoguo An
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  2. Qi Zheng
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  3. Yilin Zhou
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Correspondence to Diaoguo An.

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An, D., Zheng, Q., Zhou, Y. et al. Molecular cytogenetic characterization of a new wheat–rye 4R chromosome translocation line resistant to powdery mildew. Chromosome Res 21, 419–432 (2013). https://doi.org/10.1007/s10577-013-9366-8

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