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Construction of a high-resolution linkage map of Rfd1, a restorer-of-fertility locus for cytoplasmic male sterility conferred by DCGMS cytoplasm in radish (Raphanus sativus L.) using synteny between radish and Arabidopsis genomes

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Abstract

Cytoplasmic male sterility caused by Dongbu cytoplasmic and genic male-sterility (DCGMS) cytoplasm and its nuclear restorer-of-fertility locus (Rfd1) with a linked molecular marker (A137) have been reported in radish (Raphanus sativus L.). To construct a linkage map of the Rfd1 locus, linked amplified fragment length polymorphism (AFLP) markers were screened using bulked segregant analysis. A 220-bp linked AFLP fragment sequence from radish showed homology with an Arabidopsis coding sequence. Using this Arabidopsis gene sequence, a simple PCR marker (A220) was developed. The A137 and A220 markers flanked the Rfd1 locus. Two homologous Arabidopsis genes with both marker sequences were positioned on Arabidopsis chromosome-3 with an interval of 2.4 Mb. To integrate the Rfd1 locus into a previously reported expressed sequence tag (EST)-simple sequence repeat (SSR) linkage map, the radish EST sequences located in three syntenic blocks within the 2.4-Mb interval were used to develop single nucleotide polymorphism (SNP) markers for tagging each block. The SNP marker in linkage group-2 co-segregated with male fertility in an F2 population. Using radish ESTs positioned in linkage group-2, five intron length polymorphism (ILP) markers and one cleaved amplified polymorphic sequence (CAPS) marker were developed and used to construct a linkage map of the Rfd1 locus. Two closely linked markers delimited the Rfd1 locus within a 985-kb interval of Arabidopsis chromosome-3. Synteny between the radish and Arabidopsis genomes in the 985-kb interval were used to develop three ILP and three CAPS markers. Two ILP markers further delimited the Rfd1 locus to a 220-kb interval of Arabidopsis chromosome-3.

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Acknowledgments

This work was supported by a grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ007992), Rural Development Administration and a grant (108169-05-01-SB010) from the Technology Development Program for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea. The authors thank Seung-Hee Kim, Ji-wha Hur, and Jeong-Ahn Yoo for their dedicated technical assistance.

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Authors and Affiliations

  1. Department of Plant Biotechnology, Biotechnology Research Institute, Chonnam National University, Gwangju, 500-757, Korea

    Youngcho Cho, Tae-Ho Han & Sunggil Kim

  2. Biotech Research Center, Dongbu Advanced Research Institute, Dongbu Hannong Co., Ltd., Daejeon, 305-708, Korea

    Young-Pyo Lee

  3. The Agricultural Genome Center, National Academy of Agricultural Science, Suwon, 441-707, Korea

    Beom-Seok Park

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  1. Youngcho Cho
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  2. Young-Pyo Lee
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Correspondence to Sunggil Kim.

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Communicated by I. Paran.

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Cho, Y., Lee, YP., Park, BS. et al. Construction of a high-resolution linkage map of Rfd1, a restorer-of-fertility locus for cytoplasmic male sterility conferred by DCGMS cytoplasm in radish (Raphanus sativus L.) using synteny between radish and Arabidopsis genomes. Theor Appl Genet 125, 467–477 (2012). https://doi.org/10.1007/s00122-012-1846-2

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