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Identification of mitochondrial genome rearrangements unique to novel cytoplasmic male sterility in radish (Raphanus sativus L.)

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Abstract

A novel cytoplasmic male-sterility (CMS) radish (Raphanus sativus L.) and its associated mitotype (DCGMS) were previously identified; however, no mtDNA fragments flanking the atp6 gene were found in the DCGMS mitotype. Unlike three other mitotypes in this study, a unique mtDNA organization, atp6–nad3–rps12, was found to be the major mtDNA structure associated with this mitotype. This organization may have arisen from short repeat sequence-mediated recombination events. The short repeat clusters involved in the mtDNA rearrangement around the atp6 gene also exist as repetitive sequences in the complete mitochondrial genomes of other members of the Brassicaceae family, including rapeseed and Arabidopsis. These sequences do not exist as repetitive elements in the mtDNA of tobacco, sugar beet, or rice. While studying the regions flanking atp6, we identified a truncated atp6 mtDNA fragment which consists of the 5′ part of the atp6 gene linked to an unidentified sequence. This mtDNA structure was present in all mitotypes; however, a single nucleotide insertion mutation leading to a frame-shift was identified only in the DCGMS mitotype. Although this truncated atp6 organization was transcribed, there was no significantly different expression between male-sterile and fertile segregating individuals from the BC1F1 population originating from a cross between male-sterile and restorer parents. Comprehensive survey of the single base-pair insertion showed that it was maternally inherited and unique to the DCGMS mitotype. Therefore, this single nucleotide polymorphism (SNP) in the coding sequence of the mtDNA will be a useful molecular marker for the detection of the DCGMS mitotype.

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Acknowledgments

This work was supported by a grant (Code#20080401034012) from BioGreen 21 Program, Rural Development Administration, and a grant (307001-05-02-SB010) from Technology Development Program for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea.

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Authors and Affiliations

  1. Biotech Research Center, Dongbu Advanced Research Institute, Dongbu HiTek Co., Ltd, Daejeon, 305-708, South Korea

    Young-Pyo Lee, Heerae Lim, YoungSoon Ahn & Soon-Kee Sung

  2. Department of Plant Biotechnology, Biotechnology Research Institute, Chonnam National University, Gwangju, 500-757, South Korea

    Sunggil Kim

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  1. Young-Pyo Lee
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  2. Sunggil Kim
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  3. Heerae Lim
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Correspondence to Soon-Kee Sung.

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Communicated by R. Hagemann.

Young-Pyo Lee and Sunggil Kim equally contributed to this work.

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Lee, YP., Kim, S., Lim, H. et al. Identification of mitochondrial genome rearrangements unique to novel cytoplasmic male sterility in radish (Raphanus sativus L.). Theor Appl Genet 118, 719–728 (2009). https://doi.org/10.1007/s00122-008-0932-y

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