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Intergeneric cytoplasmic hybridization in cruciferae by protoplast fusion

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Summary

Rapeseed plants have been regenerated after fusion between protoplasts bearing cytoplasms of different genera. Cybrids combine, in a first experiment Brassica napus chloroplasts and a cytoplasmic male sterility (cms) trait coming from Raphanus sativus, in a second experiment chloroplasts of a triazine resistant Brassica campestris and cms trait from Raphanus sativus. Transfer of chloroplasts has been confirmed by restriction cp DNA analysis and two dimensional thylakoïd protein electrophoresis. These plants may be very useful for Brassica hybrid seed production.

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References

  • Aviv D, Galun E (1980) Restoration of fertility in cytoplasmic male sterile (CMS) Nicotiana sylvestris by fusion with X irradiated N. tabacum protoplasts. Theor Appl Genet 58:121–127

    Google Scholar 

  • Bannerot H, Boulidard L, Cauderon Y, Tempe J (1974) Transfer of cytoplasmic male sterility from Raphanus sativus to Brassica oleracea. Proc Eucarpia Meeting Cruciferae: 52–54

  • Bannerot H, Boulidard L, Chupeau Y (1977) Unexpected difficulties met with the radish cytoplasm. Eucarpia Cruciferae Newsletter: 2–16

  • Belliard G, Pelletier G, Vedel F, Quetier F (1978) Morphological characteristics and chloroplast DNA distribution in different cytoplasmic parasexual hybrids of Nicotiana tabacum. Mol Gen Genet 165:231–237

    Google Scholar 

  • Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature 281:401–403

    Google Scholar 

  • Beversdorf WD, Weiss-Lerman J, Erickson LR, Souza-Machado V (1980) Transfer of cytoplasmically inherited triazine resistance from birds-rape to cultivated oil seed rape (B. campestris and B. napus). Can J Genet Cytol 22:167–172

    Google Scholar 

  • Binding H, Jain SM, Finger J, Mordhorst G, Nehls R, Gressel J (1982) Somatic hybridization of an atrazine-resistant biotype of Solanum nigrum with Solanum tuberberosum. Theor Appl Genet 63:273–277

    Google Scholar 

  • Chupeau Y, Missonier C, Hommel MC, Goujaud J (1978) Somatic hybrids of plants by fusion of protoplasts. Mol Gen Genet 165:239–245

    Google Scholar 

  • Dosba F, Doussinault G (1978) Création de lignées de blé présentant les charactéristiques agronomiques favorables d'Aegilops ventricosa. Ann Amelior Plant 23:27–44

    Google Scholar 

  • Edwardson JR (1970) Cytoplasmic male sterility. Bot Rev 36:341–420

    Google Scholar 

  • Galun E, Arzee-Gonen P, Fluhr R, Edelman M, Aviv D (1982) Cytoplasmic hybridization in Nicotiana: Mitochondrial DNA analysis of progenies resulting from fusion between protoplasts having different organelle constitutions. Mol Gen Genet 186:50–56

    Google Scholar 

  • Gleba YY (1978) Extranuclear inheritance investigated by somatic hybridization. In: Thorpe TA (ed) Frontiers of plant tissue culture. Int Assoc for Plant Tissue Culture, Calgary, Alta, Canada, pp 95–102

    Google Scholar 

  • Glimelius K, Chen K, Bonnett HT (1981) Somatic hybridization in Nicotiana: segregation of organellar traits among hybrid and cybrid plants. Planta 153:504–510

    Google Scholar 

  • Grun P (1976) In: Cytoplasmic genetics and evolution, Columbia University Press, New York

    Google Scholar 

  • Kartha KK, Michayluck MR, Kao KN, Gamborg OL, Constabel F (1974) Callus formation and plant regeneration from mesophyll protoplasts of rape plants (Brassica L. cv. Zephyr). Plant Sci Lett 3:265–271

    Google Scholar 

  • Kohlenbach HW, Wenzel G, Hoffman F (1982) Regeneration of Brassica napus plantlets in cultures from isolated protoplasts of haploid stem embryos as compared with leaf protoplasts. Z Pflanzenphysiol 105:131–141

    Google Scholar 

  • Li LC, Kohlenbach HW (1982) Somatic embryogenesis in quite a direct way in cultures of mesophyll protoplasts of Brassica napus L. Plant Cell Report 1:209–211

    Google Scholar 

  • Lien S, Racker E (1971) Preparation and assay of chloroplast coupling factor CF1. In: San Pietro A (ed) Methods in enzymology, vol 23. Academic Press, New York, pp 547–556

    Google Scholar 

  • Maltais B, Bouchard CJ (1978) Une moutarde des oiseaux (B. rapa L.) résistante a l'atrazine. Phytoprotection 59:117–119

    Google Scholar 

  • McCollum GD (1981) Induction of an alloplasmic male sterile Brassica oleracea by substituting cytoplasm from “early scarlet globe” radish (Raphanus sativus). Euphytica 30:855–859

    Google Scholar 

  • Mesquida J, Renard M (1978) The entomophilous pollination of male sterile strains of Winter rapeseed (Brassica napus L) and a preliminary study of alternating devices. In: Caron TA (ed) IVth Symp Intern on Pollination, Washington, Maryland, USA, 49–57

  • Nagy F, Török I, Maliga P (1981) Extensive rearrangements in the mitochondrial DNA in somatic hybrids of Nicotiana tabacum and Nicotiana knightiana. Mol Gen Genet 183:437–439

    Google Scholar 

  • Ogura H (1968) Studies of the new male sterility in japanese radish, with special reference to the utilization of this sterility towards the practical raising of hybrid seeds. Mem Fac Agric Kagoshima University 6:39–78

    Google Scholar 

  • Primard C (1981) Induction de variabilité cytoplasmique chez Brassica napus: dissociation du caractère de déficience chlorophyllienne thermosensible de la stérilité mâle applicable en sélection par fusion somatique: mise au point de la technique. Thèse Université Paris XI Orsay

  • Remy R, Ambard-Bretteville F (1983) Two dimensional analysis of chloroplast proteins from normal and cytoplasmic male sterile Brassica napus. Theor Appl Genet 64:249–253

    Google Scholar 

  • Rousselle P (1981) Etude de systèmes d'androstérilité chez le colza (Brassica napus). Thèse univ, Rennes I

  • Schenck HR, Röbbelen G (1982) Somatic hybrids by fusion of protoplasts from Brassica oleracea and B. campestris. Z Pflanzenzuecht 89:278–288

    Google Scholar 

  • Souza-Machado VS, Bandeen JD, Stephenson GR, Lavigne P (1978) Uniparental inheritance of chloroplast atrazine tolerance in Brassica campestris. Can J Plant Sci 58:977–981

    Google Scholar 

  • Stubbe W, Herrmann RG (1982) Selection and maintenance of plastome mutants and interspecific genome/plastome hybrids from Oenothera. In: Edelman M, Hallick RB, Chua NH (eds) Methods in chloroplast molecular biology. Elsevier Biomedical Press, Amsterdam, pp 149–165

    Google Scholar 

  • Thies W (1974) New methods for the analysis of rapeseed constituents. In: Proceedings IVth International Rapeseed Conference. Giessen, West Germany. 4–8 June 1974: 275–282

  • Thomas E, Hoffmann F, Potrykus I, Wenzel G (1976) Protoplast regeneration and stem embryogenesis of haploid androgenetic rape. Mol Gen Genet 145:245–247

    Google Scholar 

  • Vedel F, Mathieu C, Lebacq P, Ambard-Bretteville F, Remy R, Pelletier G, Renard M, Rouselle P (1982) Comparative macromolecular analysis of the cytoplasms of normal and cytoplasmic male sterile Brassica napus. Theor Appl Genet 62:255–262

    Google Scholar 

  • Vedel F, Mathieu C (1983) Physical and gene mapping of chloroplast DNA from normal and cytoplasmic male sterile (radish cytoplasm) lines of Brassica napus. Current Genetics 7:13–20

    Google Scholar 

  • Xu ZH, Davey MR, Cocking EC (1982) Plant regeneration from root protoplasts of Brassica. Plant Sci Lett 24:117–121

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Biologie Cellulaire, CNRA, 78000, Versailles, France

    G. Pelletier

  2. Station d'Amélioration des Plantes, INRA, F-35650, Rennes-Le Rheu, France

    C. Primard,  Rousselle & M. Renard

  3. Laboratoire de Photosynthèse, CNRS, F-91190, Gif/Y vette, France

    F. Vedel, P. Chetrit & R. Remy

Authors
  1. G. Pelletier
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  2. C. Primard
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  3. F. Vedel
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  4. P. Chetrit
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  5. R. Remy
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  6. Rousselle
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  7. M. Renard
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Communicated by R. Herrmann

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Pelletier, G., Primard, C., Vedel, F. et al. Intergeneric cytoplasmic hybridization in cruciferae by protoplast fusion. Mol Gen Genet 191, 244–250 (1983). https://doi.org/10.1007/BF00334821

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  • DOI: https://doi.org/10.1007/BF00334821

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