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Molecular mapping in oil radish (Raphanus sativus L.) and QTL analysis of resistance against beet cyst nematode (Heterodera schachtii)

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Abstract

The beet cyst nematode (Heterodera schachtii Schmidt) can be controlled biologically in highly infected soils of sugar beet rotations using resistant varieties of oil radish (Raphanus sativus L. ssp. oleiferus DC.) as a green crop. Resistant plants stimulate infective juveniles to invade roots, but prevent them after their penetration to complete the life cycle. The resistance trait has been transferred successfully to susceptible rapeseed by the addition of a complete radish chromosome. The aim of the study was to construct a genetic map for radish and to develop resistance-associated markers. The map with 545 RAPD, dpRAPD, AFLP and SSR markers had a length of 1,517 cM, a mean distance of 2.8 cM and consisted of nine linkage groups having sizes between 120 and 232 cM. Chromosome-specific markers for the resistance-bearing chromosome d and the other eight radish chromosomes, developed previously from a series of rapeseed-radish addition lines, were enclosed as anchor markers. Each of the extra chromosomes in the addition lines could be unambiguously assigned to one of the radish linkage groups. The QTL analysis of nematode resistance was realized in the intraspecific F2 mapping population derived from a cross between varieties ‘Pegletta’ (nematode resistant) x ‘Siletta Nova’ (susceptible). A dominant major QTL Hs1Rph explaining 46.4% of the phenotypic variability was detected in a proximal position of chromosome d. Radish chromosome-specific anchor markers with known map positions were made available for future recombination experiments to incorporate segments carrying desired genes as Hs1Rph from radish into rapeseed by means of chromosome addition lines.

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Acknowledgments

We thank M. Schlathölter (P·H. Petersen Saatzucht Lundsgaard) for supplying infection material and A. Garve, U. Höfling and A. Schneider for excellent technical assistance.

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Authors and Affiliations

  1. Julius Kühn Institute, Institute for Breeding Research on Horticultural and Fruit Crops, Erwin-Baur-Straße 27, 06484, Quedlinburg, Germany

    Holger Budahn & Herbert Peterka

  2. Horticulture Department, Faculty of Agriculture, Assiut University, Assiut, 71526, Egypt

    Magdi Ali Ahmed Mousa

  3. Beijing Vegetable Research Centre, Academy of Agriculture and Forestry Sciences, 100097, Beijing, China

    Yunhua Ding

  4. Yunnan Academy of Agricultural Sciences, Biotechnology Research Institute, 650223, Kunming, China

    Shaosong Zhang & Jinbin Li

Authors
  1. Holger Budahn
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  2. Herbert Peterka
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  3. Magdi Ali Ahmed Mousa
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  4. Yunhua Ding
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  5. Shaosong Zhang
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  6. Jinbin Li
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Correspondence to Holger Budahn.

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Communicated by I. Rajcan.

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Budahn, H., Peterka, H., Mousa, M.A.A. et al. Molecular mapping in oil radish (Raphanus sativus L.) and QTL analysis of resistance against beet cyst nematode (Heterodera schachtii). Theor Appl Genet 118, 775–782 (2009). https://doi.org/10.1007/s00122-008-0937-6

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  • DOI: https://doi.org/10.1007/s00122-008-0937-6

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