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Fine mapping of a dominant thermo-sensitive genic male sterility gene (BntsMs) in rapeseed (Brassica napus) with AFLP- and Brassica rapa-derived PCR markers

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Abstract

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A new thermo-sensitive dominant genic male sterility (TSDGMS) line of Brassica napus was found and mapped in this paper. Our result will greatly accelerate the map-based cloning of the BntsMs gene.

Abstract

TE5A is a thermo-sensitive dominant genic male sterility line originating from spontaneous mutation of the inbred line TE5 in Brassica napus and provides a promising system for the development of hybrid cultivars. Genetic analysis has revealed that the BntsMs mutant is controlled by a single, dominant gene. Here, we describe the fine mapping of BntsMs using amplified fragment length polymorphism (AFLP) and intron polymorphism (IP) methodologies. We screened 1,024 primer combinations and then identified five AFLP markers linked to the BntsMs gene, two of which were successfully converted into sequence-characterised amplified region (SCAR) markers. The linkage of the markers was identified by analysing a large BC2 population of 700 recessive-fertility individuals. Two SCAR markers were found in the flanking region of the BntsMs gene at distance of 3.5 and 4.8 cm. Based on sequence information from the previously screened AFLP markers and on genome organisation comparisons of the A genome of Brassica rapa and Arabidopsis, seven IP markers linked to the BntsMs gene were developed. By analysing the 700 recessive-fertility individuals, two IP markers, IP004 and IP470, were localised to the flanking region of the BntsMs gene at a distance of 0.3 and 0.2 cm, respectively. A comparison of the B. rapa and Arabidopsis genomes revealed 27 genes of B. rapa in the flanking region of these two IP markers. It is likely that the molecular markers developed from these investigations will greatly accelerate the positional cloning of the BntsMs gene.

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Acknowledgments

This study is supported by the National Natural Science Foundation of China (31100190).

Conflict of interest

The authors have declared that no conflict of interest exists.

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Authors and Affiliations

  1. Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, No. 2 Xudong Second Road, Wuhan, 430062, People’s Republic of China

    Xinhua Zeng, Wenpin Li, Yuhuan Wu, Fang Liu, Junling Luo, Yinglong Cao, Li Zhu, Yunjing Li, Jun Li, Qingbo You & Gang Wu

  2. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture , No. 2 Xudong Second Road, Wuhan, 430062, People’s Republic of China

    Xinhua Zeng, Wenpin Li, Yuhuan Wu, Fang Liu, Junling Luo, Yinglong Cao, Li Zhu, Yunjing Li, Jun Li, Qingbo You & Gang Wu

Authors
  1. Xinhua Zeng
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  2. Wenpin Li
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  3. Yuhuan Wu
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  4. Fang Liu
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  5. Junling Luo
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  6. Yinglong Cao
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  7. Li Zhu
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  8. Yunjing Li
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  9. Jun Li
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  10. Qingbo You
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  11. Gang Wu
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Corresponding author

Correspondence to Gang Wu.

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Communicated by Istvan Rajcan.

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Zeng, X., Li, W., Wu, Y. et al. Fine mapping of a dominant thermo-sensitive genic male sterility gene (BntsMs) in rapeseed (Brassica napus) with AFLP- and Brassica rapa-derived PCR markers. Theor Appl Genet 127, 1733–1740 (2014). https://doi.org/10.1007/s00122-014-2335-6

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  • DOI: https://doi.org/10.1007/s00122-014-2335-6

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