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QTL mapping of clubroot resistance in radish (Raphanus sativus L.)

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Abstract

A QTL analysis for clubroot resistance (CR) of radish was performed using an F2 population derived from a crossing of a CR Japanese radish and a clubroot-susceptible (CS) Chinese radish. F3 plants obtained by selfing of F2 plants were used for the CR tests. The potted seedlings were inoculated and the symptom was evaluated 6 weeks thereafter. The mean disease indexes of the F3 plants were used for the phenotype of the F2. The results of two CR tests were analyzed for the presence of QTL. A linkage map was constructed using AFLP and SSR markers; it spanned 554 cM and contained 18 linkage groups. A CR locus was observed in the top region of linkage group 1 in two tests. Therefore, the present results suggest that a large part of radish CR is controlled by a single gene or closely linked genes in this radish population, although minor effects of other genomic areas cannot be ruled out. The CR locus was named Crs1. Markers linked to Crs1 showed sequence homology to the genomic region of the top of chromosome 3 of Arabidopsis, as in the case of Crr3, a CR locus in Brassica rapa. These markers should be useful for breeding CR cultivars of radish. As Japanese radishes are known to be highly resistant or immune to clubroot, these markers may also be useful in the introgression of this CR gene to Brassica crops.

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Acknowledgments

The authors thank Ms. H. Kasaoka for her excellent technical support. This work was partly supported by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (No. 17380006) and the Program for Promotion of Basic and Applied Research for Innovations in the Bio-oriented Industry (BRAIN) to M.H.

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Authors and Affiliations

  1. Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto Prefectural Institute of Agricultural Biotechnology, 74 Oji, Kitainayazuma, Seika, Soraku, Kyoto, 619-0244, Japan

    Akito Kamei, Nakao Kubo & Masashi Hirai

  2. Faculty of Agriculture, Meijo University, Shiogamaguchi, Tenpaku, Nagoya, 468-8502, Japan

    Masato Tsuro

  3. Division of Animal Sciences, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba, Ibaraki, 305-0901, Japan

    Takeshi Hayashi

  4. Graduate School of Life and Environmental Sciences, The University of Tsukuba, Tsukuba, 305-8572, Japan

    Ning Wang & Tatsuhito Fujimura

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  1. Akito Kamei
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  2. Masato Tsuro
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  3. Nakao Kubo
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  4. Takeshi Hayashi
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  5. Ning Wang
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  6. Tatsuhito Fujimura
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  7. Masashi Hirai
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Corresponding author

Correspondence to Masashi Hirai.

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Communicated by M. Havey.

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Kamei, A., Tsuro, M., Kubo, N. et al. QTL mapping of clubroot resistance in radish (Raphanus sativus L.). Theor Appl Genet 120, 1021–1027 (2010). https://doi.org/10.1007/s00122-009-1230-z

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  • DOI: https://doi.org/10.1007/s00122-009-1230-z

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