Skip to main content
SpringerLink
Log in

Utilization of palm oil decanter cake as a novel substrate for biosurfactant production from a new and promising strain of Ochrobactrum anthropi 2/3

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

A biosurfactant-producing bacterium, isolate 2/3, was isolated from mangrove sediment in the south of Thailand. It was evaluated as a potential biosurfactant producer. The highest biosurfactant production (4.52 g/l) was obtained when the cells were grown on a minimal salt medium containing 25 % (v/v) palm oil decanter cake and 1 % (w/v) commercial monosodium glutamate as carbon and nitrogen sources, respectively. After microbial cultivation at 30 °C in an optimized medium for 96 h, the biosurfactant produced was found to reduce the surface tension of pure water to 25.0 mN/m with critical micelle concentrations of 8.0 mg/l. The stability of the biosurfactant at different salinities, pH and temperature and also its emulsifying activity was investigated. It is an effective surfactant at very low concentrations over a wide range of temperatures, pH and salt concentrations. The biosurfactant obtained was confirmed as a glycolipid type biosurfactant by using a biochemical test, fourier-transform infrared spectroscopy, MNR and mass spectrometry. The crude biosurfactant showed a broad spectrum of antimicrobial activity and also had the ability to emulsify oil and enhance polyaromatic hydrocarbons solubility.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Abbasi H, Hamedi MM, Lotfabad TB, Zahiri HS, Sharafi H, Masoomi F, Moosavi-Movahedi AA, Ortiz A, Amanlou M, Noghabi KA (2012) Biosurfactant-producing bacterium, Pseudomonas aeruginosa MA01 isolated from spoiled apples: physicochemical and structural characteristics of isolated biosurfactant. J Biosci Bioeng 113:211–219

    Article  CAS  Google Scholar 

  • Abdel-Mawgoud AM, Lepine F, Deziel E (2010) Rhamnolipids: diversity of structures, microbial origins and roles. Appl Microbiol Biotechnol 86:1323–1336

    Article  CAS  Google Scholar 

  • Abubakr AR, Alimon AR, Yaakub H, Abdullah N, Ivan M (2013) Growth, nitrogen metabolism and carcass composition of goats fed palm oil by-products. Small Rumin Res 112:91–96

    Article  Google Scholar 

  • Assadi MM, Tabatabaee MS (2010) Biosurfactants and their use in upgrading petroleum vacuum distillation residue: a review. Int J Environ Res 4:549–572

    Google Scholar 

  • Banat IM, Franzetti A, Gandolfi I, Bestetti G, Martinotti MG, Fracchia L, Smyth TJ, Marchant R (2010) Microbial biosurfactants production, applications and future potential. Appl Microbiol Biotechnol 87:427–444

    Article  CAS  Google Scholar 

  • Barber WP, Stuckey DC (2000) Nitrogen removal in a modified anaerobic baffled reactor (ABR): 1, denitrification. Water Res 10:2413–2422

    Article  Google Scholar 

  • Barkay T, Navon-Venezia S, Ron EZ, Rosenberg E (1999) Enhancement of solubilization and biodegradation of polyaromatic hydrocarbons by the bioemulsifier alasan. Appl Environ Microbiol 65:2697–2702

    CAS  Google Scholar 

  • Chavalparit O, Rulkens WH, Mol APJ, Khaodhair S (2006) Options for environmental sustainability of the crude palm oil industry in Thailand through enhancement of industrial ecosystems. Environ Dev Sustain 8:271–287

    Article  Google Scholar 

  • Cooper DG, Goldenberg BG (1987) Surface-active agents from two Bacillus species. Appl Environ Microbiol 53:224–229

    CAS  Google Scholar 

  • Costa SGVAO, Nitschke M, Lepine F, Deziel E, Contiero J (2010) Structure, properties and applications of rhamnolipids produced by Pseudomonas aeruginosa L2-1 from cassava wastewater. Process Biochem 45:151–1516

    Google Scholar 

  • Das P, Mukherjee S, Sen R (2009) Biosurfactant of marine origin exhibiting heavy metal remediation properties. Bioresour Technol 100:4887–4890

    Article  CAS  Google Scholar 

  • de Lima CJB, Ribeiro EJ, Servulo EFC, Resende MM, Cardoso VL (2009) Biosurfactant production by Pseudomonas aeruginosa grown in residual soybean oil. Appl Biochem Biotechnol 152:156–168

    Article  Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  • Folch JM, Lees M, Stanly HS (1956) A simple method for the isolation and quantification of total lipids from animal tissues. J Biol Chem 226:497–509

    Google Scholar 

  • Fracchia L, Cavallo M, Martinotti MG, Banat IM (2012) Chap. 14. In: Ghista DN (ed) Biosurfactants and bioemulsifiers biomedical and related applications-present status and future potentials, biomedical science, engineering and technology, ISBN: 978-953-307-471-9, InTech pp 325–370

  • Gudina EJ, Rocha V, Teixeira JA, Rodrigues LR (2010) Antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp paracasei A20. Lett Appl Microbiol 50:419–424

    Article  CAS  Google Scholar 

  • Haron K, Mohammed AT, Halim RM, Din AK (2008) Palm-based bio-fertilizer from decanter cake and boiler ash of palm oil mill. Malaysian Palm Oil Board (MPOB). Information Series (MPOB TT No. 412):1-4

  • Intergovernmental Oceanographic Commission Manuals and Guide No.13 (1982). Manual for monitoring oil and dissolved/dispersed petroleum hydrocarbons in marine waters and beaches. UNESCO, Tokyo, Japan

  • Kim J, Vipulanandan C (2006) Removal of lead from contaminated water and clay soil using a biosurfactant. J Env Engg 132:777–786

    Article  CAS  Google Scholar 

  • Levinson MI (2009) Present realities and future perspectives. In: Zoller U, Sosis P (eds) Handbook of detergents:production. Taylor and Francis, New York, pp 1–37

    Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  • Makkar RS, Rockne KL (2003) Comparison of synthetic surfactants and biosurfactants in enhancing biodegradation of polycyclic aromatic hydrocarbons. Environ Toxicol Chem 22:2280–2292

    Article  CAS  Google Scholar 

  • Maneerat S (2009) Biosurfactants from marine microorganisms. Songklanakarin J Sci Technol 27:1263–1272

    Google Scholar 

  • Miller RM, Bartha R (1989) Evidence from liposome encapsulation for transport-limited microbial metabolism of solid alkanes. Appl Environ Microbiol 55:269–274

    CAS  Google Scholar 

  • Mulligan CN, Yong CN, Gibbs BF (2001) Heavy metal removal from sediments by biosurfactants. J Hazard Mater 85:111–125

    Article  CAS  Google Scholar 

  • Nawawi WMFW, Jamal P, Alam MZ (2010) Utilization of sludge palm oil as a novel substrate for biosurfactant production. Bioresour Technol 101:9241–9247

    Article  Google Scholar 

  • Pacwa-Plociniczak M, Plaza GA, Piotrowska-Seget Z, Cameotra SS (2011) Environmental applications of biosurfactants: recent advances. Int J Mol Sci 12:633–654

    Article  CAS  Google Scholar 

  • Praveesh BV, Soniyamby AR, Mariappan C, Kavithakumari P, Palaniswamy M, Lalitha S (2011) Biosurfactant production by Pseudomonas sp. from soil using whey as carbon source. N Y Sci J 4:99–103

    Google Scholar 

  • Prieto LM, Michelon M, Burkert JFM, Kalil SJ, Burkert CAV (2008) The production of rhamnolipid by a Pseudomonas aeruginosa strain isolated from a southern coastal zone in Brazil. Chemosphere 71:1781–1785

    Article  CAS  Google Scholar 

  • Ramani K, Jain CS, Mandal AB, Sekaran G (2012) Microbial induced lipoprotein biosurfactant from slaughterhouse lipid waste and its application to the removal of metal ions from aqueous solution. Colloid Surf B 97:254–263

    Article  CAS  Google Scholar 

  • Reis FASL, Servulo FC, de Franc FA (2004) Lipopeptide surfactant production by Bacillus subtilis grown on low-cost raw materials. Appl Biochem Biotechnol 113–116:899–912

    Article  Google Scholar 

  • Rodrigues L, Teixeira J, Oliveira R, Mei HCV (2006) Response surface optimization of the medium components for the production of biosurfactants by probiotic bacteria. Process Biochem 41:1–10

    Article  CAS  Google Scholar 

  • Saimmai A, Sobhon V, Maneerat S (2011) Molasses a whole medium for bosurfactants production by Bacillus strains and their application. Appl Biochem Biotech 165:315–335

    Article  CAS  Google Scholar 

  • Saimmai A, Rukadee O, Onlamool T, Sobhon V, Maneerat S (2012a) An efficient biosurfactant-producing bacterium Selenomonas ruminantium CT2, isolated from mangrove sediment in south of Thailand. World J Microbiol Biotechnol 29:87–102

    Article  Google Scholar 

  • Saimmai A, Rukadee O, Onlamool T, Sobhon V, Maneerat S (2012b) Isolation and functional characterization of a biosurfactant produced by a new and promising strain of Oleomonas sagaranensis AT18. World J Microbiol Biotechnol 28:2973–2986

    Article  CAS  Google Scholar 

  • Saimmai A, Sobhon V, Maneerat S (2012c) Production of biosurfactant from a new and promising strain of Leucobacter komagatae 183. Ann Microbiol 62:391–402

    Article  CAS  Google Scholar 

  • Saimmai A, Sobhon V, Rukadee O, Maneerat S (2012d) Comparison of biosurfactants produced by Bacillus subtilis TD4 and Pseudomonas aeruginosa SU7 for microbial surfactant-enhanced oil recovery. J Sci Ind Res 71:396–406

    CAS  Google Scholar 

  • Saimmai A, Tani A, Sobhon V, Maneerat S (2012e) Mangrove sediment, a new source of potential biosurfactant producing bacteria. Ann Microbiol 62:1669–1679

    Article  CAS  Google Scholar 

  • Saimmai A, Kaewrueng J, Maneerat S (2012f) Used lubricating oil degradation and biosurfactant production by SC-9 consortia obtained from oil contaminated soil. Ann Microbiol 62:1757–1767

    Article  CAS  Google Scholar 

  • Saimmai A, Rukadee O, Onlamool T, Sobhon V, Maneerat S (2013) Characterization and phylogenetic analysis of microbial surface active compounds-producing bacteria. Appl Biochem Biotechnol 168:1003–1018

    Article  Google Scholar 

  • Sanchez M, Aranda FJ, Espuny MJ, Marques A, Teruel JA, Manresa A, Ortiz A (2007) Aggregation behavior of a dirhamnolipid biosurfactant secreted by Pseudomonas aeruginosa in aqueous media. J Colloid Interface Sci 307:246–253

    Article  CAS  Google Scholar 

  • Shavandi M, Mohebali G, Haddadi A, Shakarami H, Nuhi A (2011) Emulsification potential of a newly isolated biosurfactant-producing bacterium, Rhodococcus sp. strain TA6. Colloids Surf B 82:4774–4782

    Article  Google Scholar 

  • Suwansukho P, Rukachisirikul V, Kawai F, Kittikun AH (2008) Production and applications of biosurfactant from Bacillus subtilis MUV4. Songklanakarin J Sci Technol 30:87–93

    Google Scholar 

  • Tran HG, Desmet T, Saerens K, Waegeman H, Vandekerckhove S, D’hooghe M, Bogaert IV, Soetaert W (2012) Biocatalytic production of novel glycolipids with cellodextrin phosphorylase. Bioresour Technol 115:84–87

    Article  CAS  Google Scholar 

  • Wu JY, Yeh KL, Lu WB, Lin CL, Chang JS (2008) Rhamnolipid production with indigenous Pseudomonas aeruginosa EM1 isolated from oil-contaminated site. Bioresour Technol 99:1157–1164

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We are grateful to Phuket Rajabhat University for providing a scholarship to AS. This work was supported by the Office of the Higher Education Commission (Thailand) and International Fundation for Science (Sweden) No. F/5204-1.

Author information

Authors and Affiliations

  1. Faculty of Agricultural Technology, Phuket Rajabhat University, Phuket, 83000, Thailand

    Atipan Saimmai

  2. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Suppasil Maneerat

  3. Faculty of Science and Technology, Suratthani Rajabhat University, Suratthani, 84100, Thailand

    Pongsak Noparat

Authors
  1. Pongsak Noparat
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Suppasil Maneerat
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Atipan Saimmai
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Atipan Saimmai.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Noparat, P., Maneerat, S. & Saimmai, A. Utilization of palm oil decanter cake as a novel substrate for biosurfactant production from a new and promising strain of Ochrobactrum anthropi 2/3. World J Microbiol Biotechnol 30, 865–877 (2014). https://doi.org/10.1007/s11274-013-1493-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-013-1493-z

Keywords

Search

Navigation