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An efficient biosurfactant-producing bacterium Selenomonas ruminantium CT2, isolated from mangrove sediment in south of Thailand

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Abstract

Biosurfactant-producing bacteria, isolate CT2, was isolated from mangrove sediment in the south of Thailand. The sequence of the 16S rRNA gene from isolate CT2 showed 100 % similarity with Selenomonas ruminantium. The highest biosurfactant production (5.02 g/l) was obtained when the cells were grown on minimal salt medium containing 15 g/l molasses and 1 g/l commercial monosodium glutamate supplemented with 1 g/l NaCl, 0.1 g/l leucine, 5 % (v/v) inoculum size at 30 °C and 150 rpm after 54 h of cultivation. The biosurfactant obtained by extraction with ethyl acetate showed high surface tension reduction (25.5 mN/m), a small CMC value (8 mg/l), thermal and pH stability with respect to surface tension reduction and emulsification activity and a high level of salt tolerance. The biosurfactant obtained was confirmed as a lipopeptide by using a biochemical test, FT-IR, MNR and mass spectrometry. The crude biosurfactant showed a broad spectrum of antimicrobial activity and also had the ability to emulsify oil and enhance PAHs solubility.

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Acknowledgments

We are grateful to Phuket Rajabhat University for providing a scholarship to AS. This research was supported by the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission.

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Authors and Affiliations

  1. Faculty of Agricultural Technology, Phuket Rajabhat University, Phuket, 83000, Thailand

    Atipan Saimmai

  2. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Theerawat Onlamool, Vorasan Sobhon & Suppasil Maneerat

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  1. Atipan Saimmai
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  2. Theerawat Onlamool
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  3. Vorasan Sobhon
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  4. Suppasil Maneerat
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Correspondence to Atipan Saimmai.

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Saimmai, A., Onlamool, T., Sobhon, V. et al. An efficient biosurfactant-producing bacterium Selenomonas ruminantium CT2, isolated from mangrove sediment in south of Thailand. World J Microbiol Biotechnol 29, 87–102 (2013). https://doi.org/10.1007/s11274-012-1161-8

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