Abstract
Consortia of used lubricating oil-degrading bacteria were isolated from oil-contaminated soil. The most active consortia in the assimilation of used lubricating oil were SC-9. This consortia contained four bacterial isolates based on culture-dependent technique. They were identified as Agrobacterium tumefaciens, Bacillus cereus, Chryseobacterium sp., and Sphingobacterium multivorum based on 16S rDNA sequence analysis. The SC-9 consortia showed 40.5% oil-degrading activity within 7 days. The addition of molasses to the liquid medium had a positive effect and there was an increase in the percentage of oil degradation and a greater in reduction surface tension. The ability of selected bacteria to degrade oil increased in a comparable trend as its biosurfactant production increased. Oil-degrading bacterial isolates that display substantial potential for production of biosurfactants can be applied in the bioremediation of soils contaminated with petroleum hydrocarbons.
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Acknowledgements
This work was supported by the TRF/BIOTEC Special Program for Biodiversity Research and Training grant BRT R_150005 and the Graduate school, Prince of Songkla University. This research project was also funded by a grant from Prince of Songkla University through Contract No. AGR5111990022S.
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Saimmai, A., Kaewrueng, J. & Maneerat, S. Used lubricating oil degradation and biosurfactant production by SC-9 consortia obtained from oil-contaminated soil. Ann Microbiol 62, 1757–1767 (2012). https://doi.org/10.1007/s13213-012-0434-7
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DOI: https://doi.org/10.1007/s13213-012-0434-7