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Molasses as a Whole Medium for Biosurfactants Production by Bacillus Strains and Their Application

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Abstract

Two types of biosurfactant (BS)-producing bacteria, Bacillus licheniformis TR7 and Bacillus subtilis SA9, were isolated from mangrove sediment in the south of Thailand. The BS production was done by using only molasses as a whole medium for growth and production. Under optimized conditions, the yields of TR7 and SA9 BS were found to be 3.30 and 3.78 g/l, respectively. It could reduce the surface tension of pure water to 28.5 and 29.5 mN/m, with the critical micelle concentrations of about 10 and 30 mg/l, respectively. Good thermal, pH, and salt stability were exhibited. Both BSs could recover oil more effectively than the two synthetic surfactants. In addition, TR7 and SA9 BS could enhance the solubility of polyaromatic hydrocarbons (PAHs). Thus, these BSs have the potential for the removal of oil and PAHs from the combined contaminated environment and facilitate its bioremediation. These studies indicate that molasses, as a renewable, relatively inexpensive and easily available resource, can be used for important biotechnological processes.

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References

  1. Banat, I. M., Makkar, R. S., & Cameotra, S. S. (2000). Applied Microbiology and Biotechnology, 53, 495–508.

    Article  CAS  Google Scholar 

  2. Barkay, T., Navon-Venezia, S., Ron, E. Z., & Rosenberg, E. (1999). Applied and Environmental Microbiology, 65, 2697–2702.

    CAS  Google Scholar 

  3. Bernard, D., Pascaline, H., & Jeremie, J. J. (1996). Marine Pollution Bulletin, 32, 734–739.

    Article  CAS  Google Scholar 

  4. Cha, M., Lee, N., Kim, M., Kim, M., & Lee, S. (2008). Bioresource Technology, 99, 2192–2199.

    Article  CAS  Google Scholar 

  5. Cooper, D. G., & Goldenberg, B. G. (1987). Applied and Environmental Microbiology, 53, 224–229.

    CAS  Google Scholar 

  6. Das, K., & Mukherjee, A. K. (2007). Process Biochemistry, 42, 1191–1199.

    Article  CAS  Google Scholar 

  7. Das, K., & Mukherjee, A. K. (2007). Bioresource Technology, 98, 1339–1345.

    Article  CAS  Google Scholar 

  8. Das, P., Mukherjee, S., & Sen, R. (2008). Substrate dependent production of extracellular biosurfactant by a marine bacterium. Bioresource Technology, 100, 1015–1019.

    Article  Google Scholar 

  9. Desai, J. D., & Banat, I. M. (1997). Microbiology and Molecular Biology Reviews, 61, 47–64.

    CAS  Google Scholar 

  10. Ghojavand, H., Vahabzadeh, F., Roayaei, E., & Shahraki, A. K. (2008). Journal of Colloid and Interface Science, 324, 172–176.

    Article  CAS  Google Scholar 

  11. Ghurye, G. L., & Vipulanandan, C. (1994). Biotechnology and Bioengineering, 44, 661–666.

    Article  CAS  Google Scholar 

  12. Ilori, M. O., Amobi, C. J., & Odocha, A. C. (2005). Chemosphere, 61, 985–992.

    Article  CAS  Google Scholar 

  13. Jacques, R. J. S., Santos, E. C., Bento, F. M., Peralba, M. C. R., Selbach, P. A., Sa, E. L. S., et al. (2005). International Biodeterioration and Biodegradation, 56, 143–150.

    Article  CAS  Google Scholar 

  14. Joshi, S., Bharucha, C., & Desai, A. J. (2008). Bioresource Technology, 99, 4603–4608.

    Article  CAS  Google Scholar 

  15. Joshi, S., Bharucha, C., Jha, S., Yadav, S., Nerurkar, A., & Desai, A. J. (2008). Bioresource Technology, 99, 195–199.

    Article  CAS  Google Scholar 

  16. Ke, L., Wan, W. Q., Wong, T. W. Y., Wong, Y. S., & Tam, N. F. Y. (2003). Chemosphere, 52, 1581–1591.

    Article  CAS  Google Scholar 

  17. Kumar, M., Leona, V., De Sisto-Materanoa, A., Ilzinsa, O. A., et al. (2006). Zeitschrift Fur Naturforschung C-A Journal of Biosciences, 61, 203–212.

    CAS  Google Scholar 

  18. Kuyukina, M. S., Ivshina, I. B., Makarov, S. O., Litvinenko, L. V., Cunningham, C. J., & Philip, J. C. (2005). Environment International, 31, 155–161.

    Article  CAS  Google Scholar 

  19. Lee, Y. K. (2006). In Y. K. Lee (Ed.), Microbial biotechnology: principles and applications (p. 28). Hackensack: World Scientific.

    Google Scholar 

  20. Li, Y. M., Haddad, N. I. A., Yang, S. X., & Mu, B. Z. (2008). International Journal of Peptide Research and Therapeutics, 14, 229–235.

    Article  CAS  Google Scholar 

  21. Luna-Velasco, M. A., Esparza-Garcia, F., Canizares-Villanueva, R. O., & Rodriguez-Vazquez, R. (2007). Process Biochemistry, 42, 310–314.

    Article  CAS  Google Scholar 

  22. Makkar, R. S., & Cameotra, S. S. (2002). Journal of Surfactants and Detergents, 5, 11–17.

    Article  CAS  Google Scholar 

  23. Maneerat, S. (2009). Songklanakarin Journal of Science and Technology, 27, 1263–1272.

    Google Scholar 

  24. Maneerat, S., & Phetrong, K. (2007). Songklanakarin Journal of Science and Technology, 29, 781–791.

    Google Scholar 

  25. Morikawa, M., Hirata, T., & Imanaka, T. (2000). Biochimica et Biophysica Acta, 1488, 211–218.

    CAS  Google Scholar 

  26. Mukherjee, A. K., & Das, K. (2005). FEMS Microbiology Ecology, 54, 479–489.

    Article  CAS  Google Scholar 

  27. Mukherjee, S., Das, P., Sivapathasekaran, C., & Sen, R. (2009). Letters in Applied Microbiology, 48, 281–288.

    Article  CAS  Google Scholar 

  28. Nilsson, W. B., & Strom, M. S. (2002). Diseases of Aquatic Organisms, 48, 175–185.

    Article  CAS  Google Scholar 

  29. Nitschke, M., & Coast, S. G. (2007). Trends in Food Science and Technology, 18, 252–259.

    Article  CAS  Google Scholar 

  30. Nitschke, M., & Pastore, G. (2006). Bioresource Technology, 97, 336–341.

    Article  CAS  Google Scholar 

  31. Obayori, O. S., Ilori, M. O., Adebusoye, S. A., Oyetibo, G. O., et al. (2009). World Journal of Microbiology & Biotechnology, 25, 1615–1623.

    Article  CAS  Google Scholar 

  32. Olivera, N. L., Commendatore, M. G., Delgado, O., & Esteves, J. L. (2003). Journal of Industrial Microbiology & Biotechnology, 30, 542–548.

    Article  CAS  Google Scholar 

  33. Pereira, D. S. T., Silva, A. L. S., & Lopez, M. Q. (2010). Acta Scientiarum Technology, 32, 33–36.

    CAS  Google Scholar 

  34. Phalakornkule, C., & Tanasupawat, S. (2006). Journal of Culture Collections, 5, 46–57.

    Google Scholar 

  35. Phetrong, K., H-Kittikun, A., & Maneerat, S. (2008). Songklanakarin Journal of Science and Technology, 29, 769–779.

    Google Scholar 

  36. Rashedi, H., Assadi, M. M., Jamshidi, E., & Bonakdarpour, B. (2006). International Journal of Environmental Science and Technology, 3, 297–303.

    CAS  Google Scholar 

  37. Rashedi, H., Assadi, M. M., Bonakdarpour, B., & Jamshidi, E. (2005). International Journal of Environmental Science and Technology, 2, 59–62.

    CAS  Google Scholar 

  38. Rashedi, H., Jamshidi, E., Assadi, M. M., & Bonakdarpour, B. (2005). International Journal of Environmental Science and Technology, 2, 121–127.

    CAS  Google Scholar 

  39. Rodrigues, L. R., Teixeira, J. A., van der Meib, H. C., & Oliveira, R. (2006). Colloids and Surfaces. B: Biointerfaces, 53, 105–112.

    Article  CAS  Google Scholar 

  40. Sangster, J. (1989). Journal of Physical and Chemical Reference Data, 18, 3.

    Article  Google Scholar 

  41. Snape, I., Ferguson, S. H., Harvey, P. M., et al. (2006). Chemosphere, 63, 89–98.

    Article  CAS  Google Scholar 

  42. Sobrinho, H. B. S., Rufino, R. D., Luna, J. M., Salgueiro, A. A., Campos-Takaki, G. M., Leite, L. F. C., et al. (2008). Process Biochemistry, 43, 912–917.

    Article  CAS  Google Scholar 

  43. Tamura, K., Dudley, J., Nei, M., & Kumar, S. (2007). Molecular Biology and Evolution, 24, 1596–1599.

    Article  CAS  Google Scholar 

  44. Thavasi, R., Jayalakshmi, S., Balasubramaniam, T., & Banat, I. M. (2008). World Journal of Microbiology & Biotechnology, 24, 917–925.

    Article  CAS  Google Scholar 

  45. Thavasi, R., Jayalakshmi, S., Balasubramanian, T., & Banat, I. M. (2007). Letters in Applied Microbiology, 45, 686–691.

    Article  CAS  Google Scholar 

  46. Thompson, J. D., Gibbons, T. J., Plewniak, F., Jeanmougin, F., & Higgins, D. G. (1997). Nucleic Acids Research, 25, 4876–4882.

    Article  CAS  Google Scholar 

  47. Willumsen, P. A. E., & Karlson, U. (1997). Biodegradation, 7, 415–423.

    Article  Google Scholar 

  48. Yin, B., Gua, J. D., & Wana, N. (2005). International Biodeterioration and Biodegradation, 56, 243–248.

    Article  CAS  Google Scholar 

  49. Zhao, B., Zhu, L., Li, W., & Chen, B. (2005). Chemosphere, 58, 33–40.

    Article  CAS  Google Scholar 

  50. Zhou, M., & Rhue, R. D. (2000). Environmental Science & Technology, 34, 1985–1990.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We are grateful to the Program Strategic Scholarships for Frontier Research Network for the Ph.D. Program Thai Doctoral degree from the Office of the Higher Education Commission, Thailand for providing a scholarship to A.S. This work was also funded by the Faculty of Agro-Industry and Graduate School, Prince of Songkla University.

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  1. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Atipan Saimmai, Vorasan Sobhon & Suppasil Maneerat

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  1. Atipan Saimmai
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  2. Vorasan Sobhon
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  3. Suppasil Maneerat
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Correspondence to Suppasil Maneerat.

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Saimmai, A., Sobhon, V. & Maneerat, S. Molasses as a Whole Medium for Biosurfactants Production by Bacillus Strains and Their Application. Appl Biochem Biotechnol 165, 315–335 (2011). https://doi.org/10.1007/s12010-011-9253-8

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