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Preliminary characterization of biosurfactant produced by a PAH-degrading Paenibacillus sp. under thermophilic conditions

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Abstract

The capacities of a biosurfactant producing and polycyclic aromatic hydrocarbon (PAH) utilizing bacterium, namely, strain 1C, isolated from an Algerian contaminated soil, were investigated. Strain 1C belonged to the Paenibacillus genus and was closely related to the specie Paenibacillus popilliae, with 16S rRNA gene sequence similarity of 98.4 %. It was able to produce biosurfactant using olive oil as substrate. The biosurfactant production was shown by surface tension (32.6 mN/m) after 24 h of incubation at 45 °C and 150 rpm. The biosurfactant(s) retained its properties during exposure to elevated temperatures (70 °C), relatively high salinity (20 % NaCl), and a wide range of pH values (2–10). The infrared spectroscopy (FTIR) revealed that its chemical structure belonged to lipopeptide class. The critical micelle concentration (CMC) of this biosurfactant was about 0.5 g/l with 29.4 mN/m. In addition, the surface active compound(s) produced by strain 1C enhanced PAH solubility and showed a significant antimicrobial activity against pathogens. In addition to its biosurfactant production, strain 1C was shown to be able to utilize PAHs as the sole carbon and energy sources. Strain 1C as hydrocarbonoclastic bacteria and its interesting surface active agent may be used for cleaning the environments polluted with polyaromatic hydrocarbons.

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Acknowledgments

This work was funded by the Algerian Ministry of Higher Education and Scientific Research by the CNEPRU project number J0100420120004. The authors would like to express their gratitude to Mr. Anouar Smaoui from the English Language Unit at the Faculty of Science of Sfax, Tunisia, for his valuable proofreading and language-polishing services. We express our thanks to Mr. Nidhal Bakkar and Mr. Hocine Boutoumi for their assistance in IR analyses and Mrs. Najla Mhiri and Mrs. Manel Chelbi for their help with molecular techniques.

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Authors and Affiliations

  1. Laboratory of Natural Products Chemistry and Biomolecules (LNSCB), University of Saad Dahlab-Blida 1, P.O. Box 270, 09000, Blida, Algeria

    Fatma Zohra Mesbaiah, Kamel Eddouaouda & Abdelmalek Badis

  2. National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd. Amirouche, P.O. Box 67, Bousmail, W. Tipaza, Algeria

    Fatma Zohra Mesbaiah & Abdelmalek Badis

  3. Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, PO Box 1177, 3018, Sfax, Tunisia

    Kamel Eddouaouda, Alif Chebbi, Dorra Hentati, Sami Sayadi & Mohamed Chamkha

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  1. Fatma Zohra Mesbaiah
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  2. Kamel Eddouaouda
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Correspondence to Fatma Zohra Mesbaiah.

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Mesbaiah, F.Z., Eddouaouda, K., Badis, A. et al. Preliminary characterization of biosurfactant produced by a PAH-degrading Paenibacillus sp. under thermophilic conditions. Environ Sci Pollut Res 23, 14221–14230 (2016). https://doi.org/10.1007/s11356-016-6526-3

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