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Transfer and mapping of a gene conferring later-growth-stage powdery mildew resistance in a tetraploid wheat accession

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Abstract

Wheat powdery mildew is a severe foliar disease and causes significant yield losses in epidemic years. Breeding and using resistant cultivars is the most widely employed strategy to curb this disease. To identify and transfer powdery mildew resistance genes in wild emmer wheat accession TA1410 into common wheat, a resistant F3 line derived from the cross of TA1410 × durum wheat line Zhongyin1320 was crossed with common wheat cultivar Yangmai158. The homozygous resistant BC5F2 lines derived from the backcross with Yangmai158 exhibited susceptibility at seedling stage and conferred increasing resistance when the plants were closer to heading stage. In two segregating BC5F3 families investigated at heading stage, the segregation of the resistance fit a 3:1 ratio, suggesting that a single dominant gene controls the resistance. This resistance gene, designated HSM1, was mapped to the 0.6-cM Xmag5825.1–Xgwm344 interval on chromosome 7AL and co-segregated with Xrga-C3 and Xrga-C6. A mapping position comparison with other powdery mildew resistance genes on this chromosome suggested that HSM1 belongs to the Pm1 resistance gene cluster. HSM1 is a useful candidate gene for resistance breeding, particularly in winter-wheat growing areas.

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References

  • Ben-David R, Xie WL, Peleg Z, Saranga Y, Dinoor A, Fahima T (2010) Identification and mapping of PmG16, a powdery mildew resistance gene derived from wild emmer wheat. Theor Appl Genet 121:499–510

    Article  CAS  PubMed  Google Scholar 

  • Blanco A, Gadaleta A, Cenci A, Carluccio AV, Abdelbacki AMM, Simeone R (2008) Molecular mapping of the novel powdery mildew resistance gene Pm36 introgressed from Triticum turgidum var. dicoccoides in durum wheat. Theor Appl Genet 116:417–425

    Article  Google Scholar 

  • Börner A, Schumann E, Fürste A, Cöster H, Leithold B, Röder M, Weber W (2002) Mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat (Triticum aestivum L.). Theor Appl Genet 105:921–936

    Article  PubMed  Google Scholar 

  • Bougot Y, Lemoine J, Pavoine MT, Guyomar’ch H, Gautier V, Muranty H, Barloy D (2006) A major QTL effect controlling resistance to powdery mildew in winter wheat at the adult plant stage. Plant Breed 125:550–556

    Article  CAS  Google Scholar 

  • Century KS, Lagman RA, Adkisson M, Morlan J, Tobias R, Schwartz K, Smith A, Love J, Roland PC, Whalen MC (1999) Developmental control of Xa21-mediated disease resistance in rice. Plant J 20:231–236

    Article  CAS  PubMed  Google Scholar 

  • Chantret N, Sourdille P, Röder M, Tavaud M, Bernard M, Doussinault G (2000) Location and mapping of the powdery mildew resistance gene MlRE and detection of a resistance QTL by bulked segregant analysis (BSA) with microsatellites in wheat. Theor Appl Genet 100:1217–1224

    Article  CAS  Google Scholar 

  • Chantret N, Mingeot D, Sourdille P, Bernard M, Jacquemin JM, Doussinault G (2001) A major QTL for powdery mildew resistance is stable over time and at two development stages in winter wheat. Theor Appl Genet 103:962–971

    Article  CAS  Google Scholar 

  • Chhuneja P, Kumar K, Stirnweis D, Hurni S, Keller B, Dhaliwal DS, Singh K (2012) Identification and mapping of two powdery mildew resistance genes in Triticum boeoticum L. Theor Appl Genet 124:1051–1058

    Article  CAS  PubMed  Google Scholar 

  • Fu BS, Chen Y, Li N, Ma HQ, Kong ZX, Zhang LX, Jia HY, Ma ZQ (2013) pmX: a recessive powdery mildew resistance gene at the Pm4 locus identified in wheat landrace Xiaohongpi. Theor Appl Genet 126:913–921

    Article  CAS  PubMed  Google Scholar 

  • Gao HD, Zhu FF, Jiang YJ, Wu JZ, Yan W, Zhang QF, Jacobi A, Cai SB (2012) Genetic analysis and molecular mapping of a new powdery mildew resistant gene Pm46 in common wheat. Theor Appl Genet 125:967–973

    Article  CAS  PubMed  Google Scholar 

  • Griffey CA, Das MK, Stromberg EL (1993) Effectiveness of adult plant resistance in reducing grain yield loss to powdery mildew in winter wheat. Plant Dis 77:618–622

    Article  Google Scholar 

  • Hayashi N, Inoue H, Kato T, Funao T, Shirota M, Shimizu T, Kanamori H, Yamane H, Hayano-Saito Y, Matsumoto T, Yano M, Takatsuji H (2010) Durable panicle blast-resistance gene Pb1 encodes an atypical CC-NBS-LRR protein and was generated by acquiring a promoter through local genome duplication. Plant J 64:498–510

    Google Scholar 

  • Hsam SLK, Zeller FJ (2002) Breeding for powdery mildew resistance in common wheat (Triticum aestivum L.). In: Bléanger RR, Bushnell WR, Dik AJ, Carver TLW (eds) The powdery mildews: a comprehensive treatise. American Phytopathological Society Press, St. Paul, pp 219–238

    Google Scholar 

  • Hua W, Liu ZJ, Zhu J, Xie CJ, Yang TM, Zhou YL, Duan XY, Sun QX, Liu ZY (2009) Identification and genetic mapping of pm42, a new recessive wheat powdery mildew resistance gene derived from wild emmer (Triticum turgidum var. dicoccoides). Theor Appl Genet 119:223–230

    Article  CAS  PubMed  Google Scholar 

  • Huang XQ, Röder MS (2004) Molecular mapping of powdery mildew resistance genes in wheat: a review. Euphytica 137:203–223

    Article  CAS  Google Scholar 

  • Ji XL, Xie CJ, Ni ZF, Yang TM, Nevo E, Fahima T, Liu ZY, Sun QX (2008) Identification and genetic mapping of a powdery mildew resistance gene in wild emmer (Triticum dicoccoides) accession IW72 from Israel. Euphytica 159:385–390

    Article  CAS  Google Scholar 

  • Keller M, Keller B, Schachermayr G, Winzeler M, Schmid JE, Stamp P, Messmer MM (1999) Quantitative trait loci for resistance against powdery mildew in a segregating wheat × spelt population. Theor Appl Genet 98:903–912

    Article  CAS  Google Scholar 

  • Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFadden H, Bossolini E, Selter LL, Keller B (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360–1362

    Article  CAS  PubMed  Google Scholar 

  • Lan CX, Liang SS, Wang ZL, Yan J, Zhang Y, Xia XC, He ZH (2009) Quantitative trait loci mapping for adult-plant resistance to powdery mildew in Chinese wheat cultivar Bainong 64. Phytopathology 99:1121–1126

    Article  PubMed  Google Scholar 

  • Lan CX, Ni XW, Yan J, Zhang Y, Xia XC, Chen XM, He ZH (2010) Quantitative trait loci mapping of adult-plant resistance to powdery mildew in Chinese wheat cultivar Lumai 21. Mol Breed 25:615–622

    Article  CAS  Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daley M, Lincoln S, Newburg L (1987) Mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  CAS  PubMed  Google Scholar 

  • Lebsock KL, Briggle LW (1974) Gene Pm5 for resistance to Erysiphe graminis f. sp. tritici in Hope wheat. Crop Sci 14:561–563

    Article  Google Scholar 

  • Li GQ, Fang TL, Zhang HT, Xie CJ, Li HJ, Yang TM, Nevo E, Fahima T, Sun QX, Liu ZY (2009) Molecular identification of a new powdery mildew resistance gene Pm41 on chromosome 3BL derived from wild emmer (Triticum turgidum var. dicoccoides). Theor Appl Genet 119:531–539

    Article  CAS  PubMed  Google Scholar 

  • Liang SS, Suenaga K, He ZH, Wang ZL, Liu HY, Wang DS, Singh RP, Sourdille P, Xia XC (2006) Quantitative trait loci mapping for adult-plant resistance to powdery mildew in bread wheat. Phytopathology 96:784–789

    Article  CAS  PubMed  Google Scholar 

  • Lillemo M, Asalf B, Singh RP, Huerta-Espino J, Chen XM, He ZH, Bjørnstad Å (2008) The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theor Appl Genet 116:1155–1166

    Article  CAS  PubMed  Google Scholar 

  • Lillemo M, Bjørnstad Å, Skinnes H (2012) Molecular mapping of partial resistance to powdery mildew in winter wheat cultivar Folke. Euphytica 185:47–59

    Article  CAS  Google Scholar 

  • Liu SX, Griffey CA, Maroof MAS (2001) Identification of molecular markers associated with adult plant resistance to powdery mildew in common wheat cultivar Massey. Crop Sci 41:1268–1275

    Article  CAS  Google Scholar 

  • Liu ZY, Sun QX, Ni ZF, Nevo E, Yang TM (2002) Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer. Euphytica 123:21–29

    Article  CAS  Google Scholar 

  • Liu ZJ, Zhu J, Cui Y, Liang Y, Wu HB, Song W, Liu Q, Yang T, Sun QX, Liu ZY (2012) Identification and comparative mapping of a powdery mildew resistance gene derived from wild emmer (Triticum turgidum var. dicoccoides) on chromosome 2BS. Theor Appl Genet 124:1041–1049

    Article  CAS  PubMed  Google Scholar 

  • Lu QX, Bjørnstad Å, Ren Y, Asad MA, Xia XC, Chen XM, Ji F, Shi JR, Lillemo M (2012) Partial resistance to powdery mildew in German spring wheat ‘Naxos’ is based on multiple genes with stable effects in diverse environments. Theor Appl Genet 125:297–309

    Article  CAS  PubMed  Google Scholar 

  • Ma ZQ, Sorrells ME, Tanksley SD (1994) RFLP markers linked to powdery mildew resistance genes Pm1, Pm2, Pm3, and Pm4 in wheat. Genome 37:871–875

    Article  CAS  PubMed  Google Scholar 

  • Ma ZQ, Wei JB, Cheng SH (2004) PCR-based markers for the powdery mildew resistance gene Pm4a in wheat. Theor Appl Genet 109:140–145

    Article  CAS  PubMed  Google Scholar 

  • Ma HQ, Kong ZX, Fu BS, Li N, Zhang LX, Jia HY, Ma ZQ (2011) Identification and mapping of a new powdery mildew resistance gene on chromosome 6D of common wheat. Theor Appl Genet 123:1099–1106

    Article  CAS  PubMed  Google Scholar 

  • Maxwell JJ, Lyerly JH, Cowger C, Marshall D, Brown-Guedira G, Murphy JP (2009) MlAG12: a Triticum timopheevii-derived powdery mildew resistance gene in common wheat on chromosome 7AL. Theor Appl Genet 117:103–115

    Google Scholar 

  • Maxwell JJ, Lyerly JH, Srnic G, Parks R, Cowger C, Marshall D, Brown-Guedira G, Murphy JP (2010) MlAB10: a Triticum turgidum subsp. dicoccoides derived powdery mildew resistance gene identified in common wheat. Crop Sci 50:2261–2267

    Article  CAS  Google Scholar 

  • McIntosh RA, Dubcovsky J, Rogers J, Morris C, Appels R, Xia XC (2011) Catalogue of gene symbols for wheat. In: KOMUGI—integrated wheat science database at http://www.shigen.nig.ac.jp/wheat/komugi/genes/download.jsp

  • Mingeot D, Chantret N, Baret PV, Dekeyser A, Boukhatem N, Sourdille P, Doussinault G, Jacquemin JM (2002) Mapping QTL involved in adult plant resistance to powdery mildew in the winter wheat line RE714 in two susceptible genetic backgrounds. Plant Breed 121:133–140

    Article  CAS  Google Scholar 

  • Miranda LM, Perugini L, Srnić G, Brown-Guedira G, Marshall D, Leath S, Murphy J (2007) Genetic mapping of a Triticum monococcum derived powdery mildew resistance gene in common wheat. Crop Sci 47:2323–2329

    Article  CAS  Google Scholar 

  • Mohler V, Zeller FJ, Wenzel G, Hsam SLK (2005) Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.). 9. Gene MlZec1 from the Triticum dicoccoides-derived wheat line Zecoi-1. Euphytica 142:161–167

    Article  CAS  Google Scholar 

  • Neu C, Stein N, Keller B (2002) Genetic mapping of the Lr20-Pm1 resistance locus reveals suppressed recombination on chromosome arm 7AL in hexaploid wheat. Genome 45:737–744

    Article  CAS  PubMed  Google Scholar 

  • Qin B, Cao AZ, Wang HY, Chen TT, You FM, Liu YY, Ji JH, Liu DJ, Chen PD, Wang XE (2011) Collinearity-based marker mining for the fine mapping of Pm6, a powdery mildew resistance gene in wheat. Theor Appl Genet 123:207–218

    Article  PubMed  Google Scholar 

  • Qiu YC, Zhou RH, Kong XY, Zhang SS, Jia JZ (2005) Microsatellite mapping of a Triticum urartu Tum. derived powdery mildew resistance gene transferred to common wheat (Triticum aestivum L.). Theor Appl Genet 111:1524–1531

    Article  CAS  PubMed  Google Scholar 

  • Reader SM, Miller TE (1991) The introduction into bread wheat of a major gene for resistance to powdery mildew from wild emmer wheat. Euphytica 53:57–60

    Article  Google Scholar 

  • Roberts JJ, Caldwell RM (1970) General resistance (slow mildewing) to Erysiphe graminis f. sp. tritici in Knox wheat. Phytopathology 60:1310

    Article  Google Scholar 

  • Rong JK, Millet E, Manisterski J, Feldman M (2000) A new powdery mildew resistance gene: introgression from wild emmer into common wheat and RFLP-based mapping. Euphytica 115:121–126

    Article  CAS  Google Scholar 

  • Santos FR, Pena SDJ, Epplen JT (1993) Genetic and population study of a Y-linked tetranucleotide repeat DNA polymorphism. Hum Genet 90:655–656

    CAS  PubMed  Google Scholar 

  • Shtienberg D (1992) Effects of foliar diseases on gas exchange processes: a comparative study. Phytopathology 82:760–765

    Article  Google Scholar 

  • Singh RP, Nelson JC, Sorrells ME (2000) Mapping Yr28 and other genes for resistance to stripe rust in wheat. Crop Sci 40:1148–1155

    Article  CAS  Google Scholar 

  • Singrün CH, Hsam SLK, Hartl L, Zeller FJ, Mohler V (2003) Powdery mildew resistance gene Pm22 in cultivar Virest is a member of the complex Pm1 locus in common wheat (Triticum aestivum L. em Thell.). Theor Appl Genet 106:1420–1424

    PubMed  Google Scholar 

  • Singrün CH, Hsam SLK, Zeller FJ, Wenzel G, Mohler V (2004) Localization of a novel recessive powdery mildew resistance gene from common wheat line RD30 in the terminal region of chromosome 7AL. Theor Appl Genet 109:210–214

    Article  PubMed  Google Scholar 

  • Spielmeyer W, McIntosh RA, Kolmer J, Lagudah ES (2005) Powdery mildew resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe rust cosegregate at a locus on the short arm of chromosome 7D of wheat. Theor Appl Genet 111:731–735

    Article  CAS  PubMed  Google Scholar 

  • Spielmeyer W, Singh RP, McFadden H, Wellings CR, Huerta-Espino J, Kong X, Appels R, Lagudah ES (2008) Fine scale genetic and physical mapping using interstitial deletion mutants of Lr34/Yr18: a disease resistance locus effective against multiple pathogens in wheat. Theor Appl Genet 116:481–490

    Article  CAS  PubMed  Google Scholar 

  • Srnić G, Murphy J, Lyerly J, Leath S, Marshall D (2005) Inheritance and chromosomal assignment of powdery mildew resistance genes in two winter wheat germplasm lines. Crop Sci 45:1578–1586

    Article  Google Scholar 

  • Wang YS, Pi LY, Chen XH, Chakrabarty PK, Jiang JD, Leon ALD, Liu GZ, Li LC, Benny U, Oard J, Ronald PC, Song WY (2006) Rice XA21 binding protein 3 is a ubiquitin ligase required for full Xa21-mediated disease resistance. Plant Cell 18:3635–3646

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Xiao MG, Song FJ, Jiao JF, Wang XM, Xu HX, Li HJ (2013) Identification of the gene Pm47 on chromosome 7BS conferring resistance to powdery mildew in the Chinese wheat landrace Hongyanglazi. Theor Appl Genet 126:1397–1403

    Article  CAS  PubMed  Google Scholar 

  • Xie CJ, Sun QX, Ni ZF, Yang T, Nevo E, Fahima T (2003) Chromosomal location of a Triticum dicoccoides-derived powdery mildew resistance gene in common wheat by using microsatellite markers. Theor Appl Genet 106:341–345

    CAS  PubMed  Google Scholar 

  • Xie WL, Ben-David R, Zeng B, Distelfeld A, Röder MS, Dinoor A, Fahima T (2012) Identification and characterization of a novel powdery mildew resistance gene PmG3M derived from wild emmer wheat, Triticum dicoccoides. Theor Appl Genet 124:911–922

    Article  CAS  PubMed  Google Scholar 

  • Xue F, Ji WQ, Wang CY, Zhang H, Yang BJ (2012) High-density mapping and marker development for the powdery mildew resistance gene PmAS846 derived from wild emmer wheat (Triticum turgidum var. dicoccoides). Theor Appl Genet 124:1549–1560

    Article  CAS  PubMed  Google Scholar 

  • Yao G, Zhang J, Yang L, Xu H, Jiang Y, Xiong L, Zhang C, Zhang Z, Ma Z, Sorrells M (2007) Genetic mapping of two powdery mildew resistance genes in einkorn (Triticum monococcum L.) accessions. Theor Appl Genet 114:351–358

    Article  CAS  PubMed  Google Scholar 

  • Zhang HT, Guan HY, Li JT, Zhu J, Xie CJ, Zhou YL, Duan XY, Yang T, Sun QX, Liu ZY (2010) Genetic and comparative genomics mapping reveals that a powdery mildew resistance gene Ml3D232 originating from wild emmer co-segregates with an NBS-LRR analog in common wheat (Triticum aestivum L.). Theor Appl Genet 121:1613–1621

    Article  PubMed  Google Scholar 

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Acknowledgments

This project was partially supported by ‘863’ program ‘Deep sequencing of A, D wheat genome and resistance functional genomics’, ‘973’ program 2009CB118300, and NSFC Funds 30025030, 30771344 and 30771165. The authors are grateful to Dr. Zhiyong Liu, China Agricultural University, for providing the RGA marker information.

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  1. The Applied Plant Genomics Laboratory of Crop Genomics and Bioinformatics Centre, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Na Li, Ziruo Wen, Jing Wang, Bisheng Fu, Jiajia Liu, Huanhuan Xu, Zhongxin Kong, Lixia Zhang, Haiyan Jia & Zhengqiang Ma

  2. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Na Li, Ziruo Wen, Jing Wang, Bisheng Fu, Jiajia Liu, Huanhuan Xu, Zhongxin Kong, Lixia Zhang, Haiyan Jia & Zhengqiang Ma

  3. College of Agricultural Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Zhengqiang Ma

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  1. Na Li
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Correspondence to Zhengqiang Ma.

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11032_2013_9983_MOESM1_ESM.doc

Supplemental Fig. 1. Positions of powdery mildew resistance genes currently mapped at the Pm1 resistance gene cluster on chromosome 7AL. The arrows indicate the telomere direction. (DOC 302 kb)

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Li, N., Wen, Z., Wang, J. et al. Transfer and mapping of a gene conferring later-growth-stage powdery mildew resistance in a tetraploid wheat accession. Mol Breeding 33, 669–677 (2014). https://doi.org/10.1007/s11032-013-9983-0

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