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Molecular identification of a new powdery mildew resistance gene Pm41 on chromosome 3BL derived from wild emmer (Triticum turgidum var. dicoccoides)

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Abstract

Powdery mildew caused by Blumeria graminis f. sp. tritici is an important wheat disease in China and other parts of the world. Wild emmer (Triticum turgidum var. dicoccoides) is the immediate progenitor of cultivated tetraploid and hexaploid wheats and thus an important resource for wheat improvement. Wild emmer accession IW2 collected from Mount Hermon, Israel, is highly resistant to powdery mildew at the seedling and adult plant stages. Genetic analysis using an F2 segregating population and F2:3 families, derived from a cross between susceptible durum cultivar Langdon and wild emmer accession IW2, indicated that a single dominant gene was responsible for the resistance of IW2. Bulked segregant and molecular marker analyses detected that six polymorphic SSR, one ISBP, and three EST-STS markers on chromosome 3BL bin 0.63–1.00 were linked to the resistance gene. Allelic variations of resistance-linked EST-STS marker BE489472 revealed that the allele was present only in wild emmer but absent in common wheat. Segregation distortion was observed for the powdery mildew resistance allele and its linked SSR markers with preferential transmission of Langdon alleles over IW2 alleles. The resistance gene was introgressed into common wheat by backcrossing and marker-assisted selection. Since no designated powdery mildew resistance gene has been found on chromosome 3BL, the resistance gene derived from wild emmer accession IW2 appears to be new one and was consequently designated Pm41.

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Acknowledgments

The Chinese Spring aneulpoid and deletion lines used in this study were originally provided by Drs. WJ Raupp and BS Gill of Wheat Genetics Resource Centre, Kansas State University, USA. The authors are grateful to Dr. R McIntosh of University of Sydney, Australia, for his improvement of the manuscript. This work was financially supported by the National Fund for Distinguished Young Scholars (30425039), National Natural Science Foundation of China (30571151, 30771341), Beijing Natural Science Foundation (6061003), and the State High Tech Programs (2006AA100102, 2006AA10Z1E9, 2006AA10Z1C4, 2006AA10A104, and 2006BAD01A02), State Transgenic Project (2008ZX08009-002), the Program of Introducing Talents of Discipline to Universities (111-2-03), and the Program for Changjiang Scholars and Innovative Research Teams in Universities.

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  1. Tilin Fang

    Present address: Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK, 74078, USA

Authors and Affiliations

  1. State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing, 100193, People’s Republic of China

    Genqiao Li, Tilin Fang, Hongtao Zhang, Chaojie Xie, Tsomin Yang, Qixin Sun & Zhiyong Liu

  2. National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Hongjie Li

  3. Institute of Evolution, University of Haifa, Mt. Carmel, Haifa, 31905, Israel

    Eviatar Nevo & Tzion Fahima

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  1. Genqiao Li
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Li, G., Fang, T., Zhang, H. et al. Molecular identification of a new powdery mildew resistance gene Pm41 on chromosome 3BL derived from wild emmer (Triticum turgidum var. dicoccoides). Theor Appl Genet 119, 531–539 (2009). https://doi.org/10.1007/s00122-009-1061-y

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