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Fine mapping of brown planthopper (Nilaparvata lugens Stål) resistance gene Bph28(t) in rice (Oryza sativa L.)

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Abstract

The brown planthopper (BPH; Nilaparvata lugens Stål) is one of the most destructive insect pests of rice (Oryza sativa L.) throughout the Asian rice-growing countries. DV85 is a BPH-resistant indica variety. A single dominance gene conferring resistance in DV85 was previously mapped on the long arm of chromosome 11. The objectives of this study were to investigate feeding behaviors of BPH on DV85 plants and fine-map the BPH resistance gene, here designated Bph28(t). A seedling bulk test was conducted to identify resistant plant reactionsvg to BPH feeding. The results showed that the resistance of DV85 functions by means of tolerance during BPH attack, rather than non-preference and antibiosis. For fine mapping, two F2 populations were developed by crossing DV85 with the susceptible japonica variety Kinmaze and indica 9311. A high-resolution genetic map harboring Bph28(t) was constructed and Bph28(t) was finally physically defined to an interval of 64.8 kb between markers Indel55 and Indel66. The fine-mapped Bph28(t) gene will facilitate marker-assisted gene pyramiding for BPH resistance.

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Acknowledgments

This work was supported by National Public Benefit for Agriculture Science and Technology Program (201003031), National Science and Technology Supporting Program of China (2011BAD35B02-02, 2013BAD01B02-16), NSFC (31000697), Jiangsu Science and Technology Development Program BE2012303, BE2013301), the Jiangsu Independence Innovation Project (CX(10)1031, CX(12)1003) and Jiangsu 333 Program (BRA2012126).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Han Wu, Yuqiang Liu, Jun He, Yanling Liu, Ling Jiang, Linlong Liu, Chunming Wang, Xianian Cheng & Jianmin Wan

  2. Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

    Jianmin Wan

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  1. Han Wu
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  2. Yuqiang Liu
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  3. Jun He
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  4. Yanling Liu
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  5. Ling Jiang
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  6. Linlong Liu
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  7. Chunming Wang
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  8. Xianian Cheng
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  9. Jianmin Wan
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Correspondence to Jianmin Wan.

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Han Wu and Yuqiang Liu have contributed equally to this work.

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Wu, H., Liu, Y., He, J. et al. Fine mapping of brown planthopper (Nilaparvata lugens Stål) resistance gene Bph28(t) in rice (Oryza sativa L.). Mol Breeding 33, 909–918 (2014). https://doi.org/10.1007/s11032-013-0005-z

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  • DOI: https://doi.org/10.1007/s11032-013-0005-z

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