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The identification of candidate rice genes that confer resistance to the brown planthopper (Nilaparvata lugens) through representational difference analysis

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Abstract

The development of rice varieties (Oryza sativa L.) that are resistant to the brown planthopper (BPH; Nilaparvata lugens Stål) is an important objective in current breeding programs. In this study, we generated 132 BC5F5 near-isogenic rice lines (NILs) by five backcrosses of Samgangbyeo, a BPH resistant indica variety carrying the Bph1 locus, with Nagdongbyeo, a BPH susceptible japonica variety. To identify genes that confer BPH resistance, we employed representational difference analysis (RDA) to detect transcripts that were exclusively expressed in one of our BPH resistant NIL, SNBC61, during insect feeding. The chromosomal mapping of the RDA clones that we subsequently isolated revealed that they are located in close proximity either to known quantitative trait loci or to an introgressed SSR marker from the BPH resistant donor parent Samgangbyeo. Genomic DNA gel-blot analysis further revealed that loci of all RDA clones in SNBC61 correspond to the alleles of Samgangbyeo. Most of the RDA clones were found to be exclusively expressed in SNBC61 and could be assigned to functional groups involved in plant defense. These RDA clones therefore represent candidate defense genes for BPH resistance.

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Acknowledgments

This work was supported, in part, by grants from the Crop Functional Genomic Center (CG1512, CG1422), the 21 Century Frontier Program, from the Biogreen 21 Program, Rural Development Administration, and from SRC for the Plant Metabolism Research Center (PMRC), Korea Science and Engineering Foundation (KOSEF) Program.

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  1. Yeongnam Agricultural Research Institute, Milyang, 627-803, South Korea

    Dong-Soo Park, Jong-Hee Lee, Song-Yi Song, Do-Yeon Kwak, Un-Sang Yeo, Nam-Soo Jeon, Soo-Kwon Park, Gihwan Yi, You-Chun Song, Min-Hee Nam & Yeon-Chung Ku

  2. Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University, Yongin, 446-701, South Korea

    Sang-Kyu Lee, Min-Young Song & Jong-Seong Jeon

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  1. Dong-Soo Park
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Correspondence to Dong-Soo Park.

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Communicated by T. Tai.

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Park, DS., Lee, SK., Lee, JH. et al. The identification of candidate rice genes that confer resistance to the brown planthopper (Nilaparvata lugens) through representational difference analysis. Theor Appl Genet 115, 537–547 (2007). https://doi.org/10.1007/s00122-007-0587-0

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