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AB-QTL analysis in winter wheat: I. Synthetic hexaploid wheat (T. turgidum ssp. dicoccoides  × T. tauschii) as a source of favourable alleles for milling and baking quality traits

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Abstract

The advanced backcross QTL (AB-QTL) strategy was utilised to locate quantitative trait loci (QTLs) for baking quality traits in two BC2F3 populations of winter wheat. The backcrosses are derived from two German winter wheat cultivars, Batis and Zentos, and two synthetic, hexaploid wheat accessions, Syn022 and Syn086. The synthetics originate from hybridisations of wild emmer (T. turgidum spp. dicoccoides) and T. tauschii, rather than from durum wheat and T. tauschii and thus allowed for the first time to test for exotic QTL effects on wheat genomes A and B in addition to genome D. The investigated quality traits comprised hectolitre weight, grain hardness, flour yield Type 550, falling number, grain protein content, sedimentation volume and baking volume. One hundred and forty-nine SSR markers were applied to genotype a total of 400 BC2F3 lines. For QTL detection, a mixed-model ANOVA was conducted, including the effects DNA marker, BC2F3 line, environment and marker × environment interaction. Overall 38 QTLs significant for a marker main effect were detected. The exotic allele improved trait performance at 14 QTLs (36.8%), while the elite genotype contributed the favourable effect at 24 QTLs (63.2%). The favourable exotic alleles were mainly associated with grain protein content, though the greatest improvement of trait performance due to the exotic alleles was achieved for the traits falling number and sedimentation volume. At the QTL on chromosome 4B the exotic allele increased the falling number by 19.6% and at the QTL on chromosome 6D the exotic allele led to an increase of the sedimentation volume by 21.7%. The results indicate that synthetic wheat derived from wild emmer × T. tauschii carries favourable QTL alleles for baking quality traits, which might be useful for breeding improved wheat varieties by marker-assisted selection.

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Acknowledgments

We would like to thank Dr. S. Seling (Federal Research Centre for Nutrition and Food (BfEL), Detmold) for the analysis of the quality traits and the cooperating plant breeders, Dr. E. Kazman (Saatzucht Josef Breun), Dr. J. Schacht (Limagrain-Nickerson), Dr. E. Ebmeyer (Lochow-Petkus) and Dr. A. Spanakakis (Fr. Strube Saatzucht) and their teams for carrying out the field experiments. We further appreciate the assistance of W. Bungert and H. Rehkopf at the Research Station Dikopshof, as well as P. Kerwer, S. Gehlen and C. Golletz for their technical assistance in the lab. We are also thankful to the editor in charge and the anonymous reviewers who substantially helped to improve the manuscript. This work was funded by the German Plant Genome Research Initiative (GABI) of the Federal Ministry of Education and Research (BMBF, project 312862).

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  1. Antje Kunert

    Present address: Chair of Plant Breeding, Technical University of Munich, Am Hochanger 2, 85350, Freising, Germany

Authors and Affiliations

  1. Institute of Crop Science and Resource Conservation, Chair of Plant Breeding, University of Bonn, Katzenburgweg 5, 53115, Bonn, Germany

    Oliver Dedeck & Jens Léon

  2. Max-Planck-Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829, Cologne, Germany

    Ali Ahmad Naz & Klaus Pillen

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Correspondence to Klaus Pillen.

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Communicated by D. Mather.

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Kunert, A., Naz, A.A., Dedeck, O. et al. AB-QTL analysis in winter wheat: I. Synthetic hexaploid wheat (T. turgidum ssp. dicoccoides  × T. tauschii) as a source of favourable alleles for milling and baking quality traits. Theor Appl Genet 115, 683–695 (2007). https://doi.org/10.1007/s00122-007-0600-7

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