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AB-QTL analysis in spring barley: II. Detection of favourable exotic alleles for agronomic traits introgressed from wild barley (H. vulgare ssp. spontaneum)

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Abstract

The objective of the present study was to identify favourable exotic Quantitative Trait Locus (QTL) alleles for the improvement of agronomic traits in the BC2DH population S42 derived from a cross between the spring barley cultivar Scarlett and the wild barley accession ISR42-8 (Hordeum vulgare ssp. spontaneum). QTLs were detected as a marker main effect and/or a marker × environment interaction effect (M × E) in a three-factorial ANOVA. Using field data of up to eight environments and genotype data of 98 SSR loci, we detected 86 QTLs for nine agronomic traits. At 60 QTLs the marker main effect, at five QTLs the M × E interaction effect, and at 21 QTLs both the effects were significant. The majority of the M × E interaction effects were due to changes in magnitude and are, therefore, still valuable for marker assisted selection across environments. The exotic alleles improved performance in 31 (36.0%) of 86 QTLs detected for agronomic traits. The exotic alleles had favourable effects on all analysed quantitative traits. These favourable exotic alleles were detected, in particular on the short arm of chromosome 2H and the long arm of chromosome 4H. The exotic allele on 4HL, for example, improved yield by 7.1%. Furthermore, the presence of the exotic allele on 2HS increased the yield component traits ears per m2 and thousand grain weight by 16.4% and 3.2%, respectively. The present study, hence, demonstrated that wild barley does harbour valuable alleles, which can enrich the genetic basis of cultivated barley and improve quantitative agronomic traits.

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References

  • Backes G, Madsen LH, Jaiser H, Stougaard J, Herz M, Mohler V, Jahoor A (2003) Localization of genes for resistance against Blumeria graminis f. sp. hordei and Puccinia graminis in a cross between a barley cultivar and a wild barley (Hordeum vulgare ssp. spontaneum) line. Theor Appl Genet 106:353–362

    PubMed  CAS  Google Scholar 

  • Baek HJ, Beharav A, Nevo E (2003) Ecological-genomic diversity of microsatellites in wild barley, Hordeum spontaneum, populations in Jordan. Theor Appl Genet 106:397–410

    PubMed  CAS  Google Scholar 

  • Barua UM, Chalmers KJ, Hackett CA, Thomas WTB, Powell W, Waugh R (1993) Identification of RAPD markers linked to a Rhynchosporium secalis resistance locus in barley using near-isogenic lines and bulked segregant analysis. Heredity 71:177–184

    Article  PubMed  CAS  Google Scholar 

  • Bernacchi D, Beck-Bunn D, Eshed T, Lopez Y, Petiard V, Uhlig J, Zamir D, Tanksley SD (1998) Advanced backcross QTL analysis in tomato. I. Identification of QTLs for traits of agronomic importance from Lycopersicon hirsutum. Theor Appl Genet 97:381–397

    Article  CAS  Google Scholar 

  • Bezant JH, Laurie DA, Pratchett N, Chojecki J, Kearsey MJ (1996) Marker regression mapping of QTL controlling flowering time and plant height in a spring barley (Hordeum vulgare L.) cross. Heredity 77:64–71

    Article  CAS  Google Scholar 

  • Bezant J, Pratchett N, Laurie D, Chojeki J, Kearsey M (1997) Mapping QTL controlling yield and yield components in a spring barley (Hordeum vulgare L.) cross using marker regression. Mol Breeding 3:29–38

    Article  CAS  Google Scholar 

  • Börner A, Korzun V, Worland AJ (1998) Comparative genetic mapping of loci affecting plant height and development in cereals. Euphytica 100:245–248

    Article  Google Scholar 

  • Brondani C, Rangel PHN, Brondani RPV, Ferreira ME (2002) QTL mapping and introgression of yield-related traits from Oryza glumaepatula to cultivated rice (Oryza sativa) using microsatellite markers. Theor Appl Genet 104:1192–1203

    Article  PubMed  CAS  Google Scholar 

  • Costa JM, Corey A, Hayes PM, Jobet C, Kleinhofs A, Kopisch-Obusch A, Kramer SF, Kudrna D, Li M, Riera-Lizarazu O, Sato K, Szucs P, Toojinda T, Vales MJ, Wolfe RI (2001) Molecular mapping of the Oregon Wolfe Barleys: a phenotypically polymorphic doubled-haploid population. Theor Appl Genet 103:415–424

    Article  CAS  Google Scholar 

  • Erkkilä MJ, Leah R, Ahokas H, Cameron-Mills V (1998) Allele-dependent barley grain β-amylase activity. Plant Physiol 117:679–685

    Article  PubMed  Google Scholar 

  • Fischbeck G, Jahoor A (1991) The transfer of genes for mildew resistance from Hordeum spontaneum. In: Jorgensen JH (ed) Integrated control of cereal mildews: virulence patterns and their change. Riso National Laboratory, Denmark, pp 247–255

    Google Scholar 

  • Franckowiak JD (1997) Revised linkage maps for morphological markers in barley, Hordeum vulgare. Barley Genet Newsl 26:9–21

    Google Scholar 

  • Fulton TM, Nelson JC, Tanksley SD (1997) Introgression and DNA marker analysis of Lycopersicon peruvianum, a wild relative of the cultivated tomato, into Lycopersicon esculentum, followed through three successive backcross generations. Theor Appl Genet 95:895–902

    Article  CAS  Google Scholar 

  • Fulton TM, Grandillo S, Beck-Bunn T, Fridman E, Frampton A, Lopez J, Petiard V, Uhlig J, Zamir D, Tanksley SD (2000) Advanced backcross QTL analysis of a Lycopersicon esculentum × Lycopersicon parviflorum cross. Theor Appl Genet 100:1025–1042

    Article  CAS  Google Scholar 

  • Fulton TM, Bucheli P, Voirol E, Lopez J, Pétiard V, Tanksley SD (2002) Quantitative trait loci (QTL) affecting sugars, organic acids and other biochemical properties possibly contributing to flavor, identified in four advanced backcross populations of tomato. Euphytica 127:163–177

    Article  CAS  Google Scholar 

  • Gottwald S, Börner A, Stein N, Sasaki T, Graner A (2004) The gibberellic-acid insensitive dwarfing gene sdw3 of barley is located on chromosome 2HS in a region that shows high colinearity with rice chromosome 7L. Mol Gen Genomics 271:426–436

    Article  CAS  Google Scholar 

  • Gur A, Zamir D (2004) Unused natural variation can lift yield barriers in plant breeding. PLOS Biol 2 (10):1610–1615

    Article  CAS  Google Scholar 

  • Haldane JBS (1947) The interaction of nature and nurture. Annals of Eugenics 13:197–205

    Google Scholar 

  • Hayes PM, Liu BH, Knapp SJ, Chen F, Jones B, Blake T, Franckowiak J, Rasmusson D, Sorrells M, Ullrich SE, Wesenberg D, Kleinhofs A (1993) Quantitative trait locus effects and environmental interaction in a sample of North American barley germplasm. Theor Appl Genet 87:392–401

    Article  Google Scholar 

  • Hayes PM, Chen FQ, Kleinhofs A, Kilian A, Mather D (1996) Barley genome mapping and its applications. In: Jauhar PP (ed) Methods of Genome Analysis in Plants. CRC Press, Boca Raton, pp 229–249

    Google Scholar 

  • Ho JC, McCouch SR, Smith ME (2002) Improvement of hybrid yield by advanced backcross QTL analysis in maize. Theor Appl Genet 105:440–448

    Article  PubMed  CAS  Google Scholar 

  • Huang XQ, Cöster H, Ganal MW, Röder MS (2003) Advanced backcross QTL analysis for the identification of quantitative trait loci alleles from wild relatives of wheat (Triticum aestivum L.). Theor Appl Genet 106:1379–89

    PubMed  CAS  Google Scholar 

  • Huang XQ, Kempf H, Ganal MW, Röder MS (2004) Advanced backcross QTL analysis in progenies derived from a cross between a German elite winter wheat variety and a synthetic wheat (Triticum aestivum L.). Theor Appl Genet 109:933–43

    Article  PubMed  CAS  Google Scholar 

  • Jefferies SP, Barr AR, Karakousis A, Kretschmer JM, Manning S, Chalmers KJ, Nelson JC, Islam AKMR, Langridge P (1999) Mapping of chromosome regions conferring boron toxicity tolerance in barley (Hordeum vulgare L.). Theor Appl Genet 98:1293–1303

    Article  CAS  Google Scholar 

  • Kandemir N, Kudrna DA, Ullrich SE, Kleinhofs A (2000) Molecular marker assisted genetic analysis of head shattering in six-rowed barley. Theor Appl Genet 101:203–210

    Article  CAS  Google Scholar 

  • Kleinhofs A, Graner A (2001) An integrated map of the barley genome. Kluwer, Dordrecht, The Netherlands, pp 187–99

    Google Scholar 

  • Komatsuda T, Maxim P, Senthil N, Mano Y (2004) High-density AFLP map of nonbrittle rachis 1 (btr1) and 2 (btr2) genes in barley (Hordeum vulgare L.). Theor Appl Genet 109:986–995

    Article  PubMed  CAS  Google Scholar 

  • von Korff M, Wang H, Léon J, Pillen K (2004) Development of candidate introgression lines using an exotic barley accession (H. vulgare ssp. spontaneum) as donor. Theor Appl Genet 109:1736–45

    Article  PubMed  CAS  Google Scholar 

  • von Korff M, Wang H, Léon J, Pillen K (2005) AB-QTL analysis in spring barley: I. Detection of resistance genes against powdery mildew, leaf rust and scald introgressed from wild barley. Theor Appl Genet (in press)

  • Laurie DA, Pratchett N, Bezant JH, Snape JW (1994) Genetic analysis of a photoperiod-response gene on the short arm of chromosome 2 (2H) of Hordeum vulgare (barley). Heredity 72:619–627

    Article  CAS  Google Scholar 

  • Laurie DA, Pratchett N, Bezant JH, Snape JW (1995) RFLP mapping of five major genes and eight quantitative trait loci controlling flowering time in a winter × spring barley (Hordeum vulgare L.) cross. Genome 38:575–585

    PubMed  CAS  Google Scholar 

  • Li JZ, Huang XQ, Heinrichs F, Ganal MW, Röder MS (2004) Analysis of QTLs for yield, yield components, and malting quality in a BC3-DH population of spring barley. Theor Appl Genet DOI10.1007/s00122–004–1847-x

  • Marquez-Cedillo LA, Hayes PM, Jones BL, Kleinhofs A, Legge WG, Rossnagel BG, Sato K, Ullrich E, Wesenberg DM (2000) QTL analysis of malting quality in barley based on the doubled-haploid progeny of two elite North American varieties representing different germplasm groups. Theor Appl Genet 1001:173–184

    Article  Google Scholar 

  • Matus I, Corey A, Filchkin T, Hayes PM, Vales MI, Kling J, Riera-Lizarazu O, Sato K, Powell W, Waugh R (2003) Development and characterization of recombinant chromosome substitution lines (RCSLs) using Hordeum vulgare subsp. spontaneum as a source of donor alleles in a Hordeum vulgare subsp. vulgare background. Genome 46:1010–1023

    Article  PubMed  CAS  Google Scholar 

  • Moncada PP, Martinez CP, Borrero J, Chatel M, Gauch H Jr, Guimaraes E, Tohme J, McCouch SR (2001) Quantitative trait loci for yield and yield components in an Oryza sativa ×  Oryza rufipogon BC2F2 population evaluated in an upland environment. Theor Appl Genet 102:41–52

    Article  CAS  Google Scholar 

  • Pillen K, Binder A, Kreuzkam B, Ramsay L, Waugh R, Förster J, Léon J (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars. Theor Appl Genet 101:652–660

    Article  CAS  Google Scholar 

  • Pillen K, Zacharias A, Léon J (2003) Advanced backcross QTL analysis in barley (Hordeum vulgare L.). Theor Appl Genet 107:340–352

    Article  PubMed  CAS  Google Scholar 

  • Pillen K, Zacharias A, Léon J (2004) Comparative AB-QTL analysis in barley using a single exotic donor of Hordeum vulgare ssp. spontaneum. Theor Appl Genet 108:1591–1601

    Article  PubMed  CAS  Google Scholar 

  • Powell W, Ellis RP, Macaulay M, McNicol J, Forster BP (1990) The effect of selection for protein and isozyme loci on quantitative traits in a doubled haploid population of barley. Heredity 65:115–122

    Article  CAS  Google Scholar 

  • Rao GU, Ben Chaim A, Borovsky Y, Paran I (2003) Mapping of yield related QTLs in pepper in an interspecific cross of Capsicum annuum and C. frutescens. Theor Appl Genet 106:1457–1466

    PubMed  CAS  Google Scholar 

  • SAS Institute (2003) The SAS system for Windows, release 9.1. SAS Institute, Cary, N.C. USA

  • Septiningsih EM, Praseiyono J, Lubis E, Tai TH, Tjubaryat T, Moeljopawiro S, McCouch SR (2003) Identification of quantitative trait loci for yield and yield components in an advanced backcross population derived from the Oryza sativa variety IR64 and the wild relative O. rufipogon. Theor Appl Genet 107:1419–32

    Article  PubMed  CAS  Google Scholar 

  • Tanksley SD, Nelson JC (1996) Advanced backcross QTL analysis: a method of the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines. Theor Appl Genet 92:191–203

    Article  Google Scholar 

  • Tanksley SD, Grandillo S, Fulton TM, Zamir D, Eshed Y, Petiard V, Lopez J, Beck-Brunn T (1996) Advanced backcross QTL analysis in a cross between an elite processing line of tomato and its wild relative L. pimpinellifolium. Theor Appl Genet 92:213–224

    Article  CAS  Google Scholar 

  • Thomas WTB, Powell W, Waugh R, Chalmers KJ, Barua UM, Jack P, Lea V, Forster BP, Swanston JS, Ellis RP, Hanson PR (1995) Detection of quantitative trait loci for agronomic, yield, grain, and disease characters in spring barley (Hordeum vulgare L). Theor Appl Genet 91:1037–1047

    Article  CAS  Google Scholar 

  • Tinker NA, Mather DE, Rossnagel BG, Kasha KJ, Kleinhofs A, Hayes PM, Falk DE, Ferguson T, Shugar LP, Legge WG, Irvine RB, Choo TM, Briggs KG, Ullrich SE, Franckowiak JD, Blake TK, Raf RJ, Dofing SM, Saghai Maroof MA, Scoles GJ, Hoffman D, Dahleen LS, Kilian A, Chen F, Biyashev RM, Kudrna DA, Steffenson BJ (1996) Regions of the genome that affect agronomic performance in two-row barley. Crop Sci 36:1053–1062

    Article  Google Scholar 

  • Turpeinen T, Tenhola T, Manninen O, Nevo E, Nissila E (2001) Microsatellite diversity associated with ecology factors in Hordeum spontaneum populations in Israel. Mol Ecol 10:1577–1591

    Article  PubMed  CAS  Google Scholar 

  • Wu J-L, Sinha PK, Variar M, Zheng K-L, Leach JE, Courtois B, Leung H (2004) Association between molecular markers and blast resistance in an advanced backcross population of rice. Theor Appl Genet 108:1024–1032

    Article  PubMed  CAS  Google Scholar 

  • Xiao J, Grandillo S, Ahn SN, McCouch SR, Tanksley SD, Li J, Yuan L (1996) Genes from wild rice improve yield. Nature 384:223–24

    Article  CAS  Google Scholar 

  • Xiao J, Li J, Grandillo S, Nag SN, Yuan L, Tanksley SD, McCouch SR (1998) Identification of trait-improving quantitative trait loci alleles from a wild rice relative, Oryza rufipogon. Genetics 150:899–909

    PubMed  CAS  Google Scholar 

  • Yin X, Stam P, Dourleijn CJ, Kropff MJ (1999) AFLP mapping of quantitative trait loci for yield-determining physiological characters in spring barley. Theor Appl Genet 99:244–253

    Article  CAS  Google Scholar 

  • Yin Y, Chasalow SD, Stam P, Kropff MJ, Dourleijn CJ, Bos J, Bindraban PS (2002) Use of component analysis in QTL mapping of complex crop traits: a case study on yield in barley. Plant Breeding 121:314–319

    Article  Google Scholar 

  • Zeller EJ (1998) Nutzung des genetischen Potentials der Hordeum-Wildarten zur Verbesserung der Kulturgerste (Hordeum vulgare L). J Appl Bot 72:162–167

    Google Scholar 

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Acknowledgements

We thank Dr. Eberhard Laubach (Nordsaat Saatzucht), Dr. Claus Einfeldt (Saatzucht Dr. J. Ackermann), Mr. Josef Breun (Saatzucht Josef Breun) and their teams for conducting the field experiments. The excellent technical assistance of Merle Noschinski, Carsten Golletz, and the team of our experimental station Dikopshof in Wesseling is appreciated. This work was funded by the German Plant Genome Research Initiative (GABI) of the Federal Ministry of Education and Research (BMBF, project 0312278A).

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  1. M. von Korff

    Present address: Department of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, Haus 26, 14476, Golm, Germany

  2. H. Wang

    Present address: Gansu Agricultural University, Yinmencun 1, 730070, Anningqu, Lanzhou, China

Authors and Affiliations

  1. Institute of Crop Science and Resource Conservation, Chair of Crop Science and Plant Breeding, University of Bonn, Katzenburgweg 5, 53115, Bonn, Germany

    M. von Korff, H. Wang, J. Léon & K. Pillen

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  1. M. von Korff
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Correspondence to K. Pillen.

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Communicated by G. Wenzel

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Korff, M.v., Wang, H., Léon, J. et al. AB-QTL analysis in spring barley: II. Detection of favourable exotic alleles for agronomic traits introgressed from wild barley (H. vulgare ssp. spontaneum). Theor Appl Genet 112, 1221–1231 (2006). https://doi.org/10.1007/s00122-006-0223-4

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