Skip to main content
SpringerLink
Log in

Validation of quantitative trait loci for grain quality-related traits in a winter wheat mapping population

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Validating positions and effects of putative quantitative trait loci (QTL) is an important step before employing linked markers in marker-assisted selection (MAS) or conducting finer-scale mapping. Previously, QTL for grain quality traits were identified in a population of 185 doubled haploid (DH) winter wheat (Triticum aestivum L.) lines developed from the cross CO940610/’Platte’. Clusters of QTL for grain quality traits were detected on chromosomes 1B, 6B, and 7B. Our objectives were to (1) confirm the previously identified QTL, using recombinant inbred line (RIL, n = 186) and BC3F2:3 (n = 35) populations developed from the same parents; and (2) test the effects of combinations of alleles at three marker loci in the target chromosome regions. Field trials were conducted in two Colorado environments for the RIL population in 2009/10 and three environments for the BC3F2:3 population in 2012/13. Most QTL previously detected for grain quality traits on 1B, 6B, and 7B were repeated in either the RIL or BC3F2:3 populations, although these QTL were not robust across populations and environments. However, the QTL for grain ash concentration (Gac) on 7B was detected in all environments and populations, with Platte contributing the higher value allele. The BC3F2:3 lines with the allelic combination predicted to have higher grain protein concentration (Gpc) surpassed the lowest predicted combination in one of three environments investigated. Based on these evaluations, marker Bx7-MAR on 1B and the regions around markers Xwmc182a on 6B and Xwmc182b on 7B are recommended for further research for improving grain quality traits.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Ahmadi A, Baker DA (2001) The effect of water stress on the activities of key regulatory enzymes of the sucrose to starch pathway in wheat. Plant Growth Regul 35:81–91

    Article  CAS  Google Scholar 

  • Ahuja I, de Vos RCH, Bones AM, Hall RD (2010) Plant molecular stress responses face climate change. Trends Plant Sci 15:664–674

    Article  CAS  PubMed  Google Scholar 

  • Bassam BJ, Caetano-Anollés G, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196:80–83

    Article  CAS  PubMed  Google Scholar 

  • Butow BJ, Gale KR, Ikea J, Juhász A, Bedö Z, Tamás L, Gianibelli MC (2004) Dissemination of the highly expressed Bx7 glutenin subunit (Glu-B1al allele) in wheat as revealed by novel PCR markers and RP-HPLC. Theor Appl Genet 109:1525–1535

    Article  CAS  PubMed  Google Scholar 

  • Campbell BT, Baenziger PS, Gill KS, Eskridge KM, Budak H, Erayman M, Dweikat I, Yen Y (2003) Identification of QTLs and environmental interactions associated with agronomic traits on chromosome 3A of wheat. Crop Sci 43:1493–1505

    Article  CAS  Google Scholar 

  • Daniel C, Triboi E (2002) Changes in wheat protein aggregation during grain development: effects of temperatures and water stress. Eur J Agron 16:1–12

    Article  CAS  Google Scholar 

  • El-Feki WM (2010) Mapping quantitative trati loci for bread making quality and agronomic traits in winter wheat under different soil moisture levels. PhD dissertation. Colorado State University, Fort Collins, CO

  • El-Feki WM, Byrne PF, Reid SD, Lapitan NLV, Haley SD (2013) Quantitative trait locus mapping for end-use quality traits in hard winter wheat under contrasting soil moisture levels. Crop Sci 53:1953–1967

    Article  Google Scholar 

  • El-Feki WM, Byrne PF, Reid SD, Haley SD (2015) Registration of CO940610/’Platte’ wheat doubled haploid mapping population. J Plant Regist 9:419–423

    Article  Google Scholar 

  • FAOSTAT (2015) Database available at http://faostat3.fao.org/browse/Q/*/E. Accessed June 1, 2016

  • Guttieri MJ, Ahmad R, Stark JC, Souza E (2000) End-use quality of six hard red spring wheat cultivars at different irrigation levels. Crop Sci 40:631–635

    Article  Google Scholar 

  • Haley SD, Quick JS, Johnson JJ, Peairs FB, Stromberger JA, Clayshulte SR, Clifford BL, Rudolph JB, Seabourn BW, Chung OK, Jin Y, Kolmer J (2005) Registration of ‘Hatcher’ wheat. Crop Sci 45:2654–2656

    Article  Google Scholar 

  • Haley SD, Johnson JJ, Peairs FB, Quick JS, Stromberger JA, Clayshulte SR, Butler JD, Rudolph JB, Seabourn BW, Bai G, Jin Y, Kolmer J (2007) Registration of “Ripper” wheat. J Plant Regist 1:1–6

    Article  Google Scholar 

  • Haley SD, Johnson JJ, Peairs FB, Stromberger JA, Heaton EE, Seifert SA, Kottke RA, Rudolph JB, Martin TJ, Bai G, Chen X, Bowden RL, Jin Y, Kolmer JA, Seifers DL, Chen MS, Seabourn BW (2011) Registration of ‘Snowmass’ wheat. J Plant Regist 5:87–90

    Article  Google Scholar 

  • Hallauer AR, Carena MJ, Miranda Filho JB (2010) Quantitative genetics in maize breeding. Springer, New York

    Google Scholar 

  • Huang XQ, Cloutier S, Lycar L, Radovanovic N, Humphreys DG, Noll JS, Somers DJ, Brown PD (2006) Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats (Triticum aestivum L.). Theor Appl Genet 113:753–766

    Article  CAS  PubMed  Google Scholar 

  • Ishikawa G, Nakamura K, Ito H, Saito M, Sato M, Jinno H, Yoshimura Y, Nishimura T, Maejima H, Uehara Y, Kobayashi F, Nakamura T (2014) Association mapping and validation of QTLs for flour yield in the soft winter wheat variety Kitahonami. PLoS ONE 9:e111337

    Article  PubMed  PubMed Central  Google Scholar 

  • Knapp SJ, Stroup WW, Ross WM (1985) Exact confidence intervals for heritability on a progeny mean basis. Crop Sci 25:192–194

    Article  Google Scholar 

  • Kojima H, Nishio Z, Kobayashi F, Saito M, Sasaya T, Kiribuchi-Otobe C, Seki M, Oda S, Nakamura T (2015) Identification and validation of a quantitative trait locus associated with wheat yellow mosaic virus pathotype I resistance in a Japanese wheat variety. Plant Breed 134:373–378

    Article  CAS  Google Scholar 

  • Li WH, Liu W, Liu L, You MS, Liu GT, Li BY (2011) QTL mapping for wheat flour color with additive, epistatic, and QTL × environmental interaction effects. Agric Sci China 10:651–660

    Article  CAS  Google Scholar 

  • Littell R, Milliken G, Stroup W, Wolfinger R, Schabenberger O (2006) Spatial variability. In: SAS for Mixed Models. 2nd edn. SAS Institute Inc., Cary, NC, pp 437–478

  • Ma H, Busch RH, Riera-Lizarazu O, Rines HW, Dill-Macky R (1999) Agronomic performance of lines derived from anther culture, maize pollination and single-seed descent in a spring wheat cross. Theor Appl Genet 99:432–436

    Article  CAS  PubMed  Google Scholar 

  • Martin TJ, Fritz AK, Seifers D, Shroyer JP (2007) RonL hard white wheat. Publication L-296. Kansas State University Agricultural Experiment Station and Cooperative Extension Service, Manhattan, KS

  • Mengistu N, Baenziger PS, Eskridge KM, Dweikat I, Wegulo SN, Gill KS, Mujeeb-Kazi A (2012) Validation of QTL for grain yield-related traits on wheat chromosome 3A using recombinant inbred chromosome lines. Crop Sci 52:1622–1632

    Article  Google Scholar 

  • Prasad M, Kumar N, Kulwal PL, Röder MS, Balyan HS, Dhaliwal HS, Gupta PK (2003) QTL analysis for grain protein content using SSR markers and validation studies using NILs in bread wheat. Theor Appl Genet 106:659–667

    Article  CAS  PubMed  Google Scholar 

  • Rampino P, Pataleo S, Gerardi C, Mita G, Perrotta C (2006) Drought stress response in wheat: physiological and molecular analysis of resistant and sensitive genotypes. Plant Cell Environ 29:2143–2152

    Article  CAS  PubMed  Google Scholar 

  • Riede CR, Anderson JA (1996) Linkage of RFLP markers to an aluminum tolerance gene in wheat. Crop Sci 36:905–909

    Article  Google Scholar 

  • Riggs TJ, Snape JW (1977) Effects of linkage and interaction in a comparison of theoretical populations derived by diploidized haploid and single seed descent methods. Theor Appl Genet 49:111–115

    Article  CAS  PubMed  Google Scholar 

  • Seleiman M, Abdel-Aal S, Ibrahim M, Zahran G (2011) Productivity, grain and dough quality of bread wheat grown with different water regimes. J Agro Crop Sci 2:11–17

    Google Scholar 

  • Simmonds J, Scott P, Leverington-Waite M, Turner AS, Brinton J, Korzun V, Snape J, Uauy C (2014) Identification and independent validation of a stable yield and thousand grain weight QTL on chromosome 6A of hexaploid wheat (Triticum aestivum L.). BMC Plant Biol 14:191

    Article  PubMed  PubMed Central  Google Scholar 

  • Snape JW (1976) A Theoretical comparison of diploidised haploid and single seed descent populations. Heredity (Edinb) 36:275–277

    Article  Google Scholar 

  • Van Ooijen JW (2006) JoinMap 4: Software for the calculation of genetic linkage maps in experimental populations.In: Kyazma BV (ed), Wageningen, The Netherlands

  • Walthall CL, Hatfield J, Backlund P, Lengnick L, Marshall E et al (2012) Climate change and agriculture in the United States: effects and adaptation. USDA Technical Bulletin 1935, Washington, DC, p 186

    Google Scholar 

  • Weightman RM, Millar S, Alava J, Foulkes MJ, Fish L, Snape JW (2008) Effects of drought and the presence of the 1BL/1RS translocation on grain vitreosity, hardness and protein content in winter wheat. J Cereal Sci 47:457–468

    Article  CAS  Google Scholar 

  • Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421

    Article  Google Scholar 

  • Zheng S, Byrne PF, Haley SD, Shan X, Reid SD (2010) Glutenin allelic variation and 1AL.1RS effects on dough mixing properties of wheat grown in irrigated and rainfed environments. Euphytica 176:357–369

    Article  CAS  Google Scholar 

  • Zhu X, Wang H, Guo J, Wu Z, Cao A, Bie T, Nie M, You FM, Cheng Z, Xiao J, Liu Y, Cheng S, Chen P, Wang X (2012) Mapping and validation of quantitative trait loci associated with wheat yellow mosaic bymovirus resistance in bread wheat. Theor Appl Genet 124:177–188

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

We are grateful to John Stromberger, who guided the wheat grain quality evaluation; Victoria Anderson, Emily Hudson-Arns, and Scott Seifert for help with planting and harvesting; and Tom Trout and Gerald Buchleiter for their assistance with trial management.

Author information

Author notes

  1. Hung Quoc Dao

    Present address: , 16/3/20 Le Van Hien St, Duc Thang ward, Bac Tu Liem district, Ha Noi, Vietnam

Authors and Affiliations

  1. Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO, 80523, USA

    Hung Quoc Dao, Patrick F. Byrne, Scott D. Reid & Scott D. Haley

Authors
  1. Hung Quoc Dao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Patrick F. Byrne
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Scott D. Reid
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Scott D. Haley
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Patrick F. Byrne.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 20 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dao, H.Q., Byrne, P.F., Reid, S.D. et al. Validation of quantitative trait loci for grain quality-related traits in a winter wheat mapping population. Euphytica 213, 5 (2017). https://doi.org/10.1007/s10681-016-1793-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10681-016-1793-0

Keywords

Search

Navigation