Skip to main content
SpringerLink
Log in

Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats (Triticum aestivum L.)

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Development of high-yielding wheat varieties with good end-use quality has always been a major concern for wheat breeders. To genetically dissect quantitative trait loci (QTLs) for yield-related traits such as grain yield, plant height, maturity, lodging, test weight and thousand-grain weight, and for quality traits such as grain and flour protein content, gluten strength as evaluated by mixograph and SDS sedimentation volume, an F1-derived doubled haploid (DH) population of 185 individuals was developed from a cross between a Canadian wheat variety “AC Karma” and a breeding line 87E03-S2B1. A genetic map was constructed based on 167 marker loci, consisting of 160 microsatellite loci, three HMW glutenin subunit loci: Glu-A1, Glu-B1 and Glu-D1, and four STS-PCR markers. Data for investigated traits were collected from three to four environments in Manitoba, Canada. QTL analyses were performed using composite interval mapping. A total of 50 QTLs were detected, 24 for agronomic traits and 26 for quality-related traits. Many QTLs for correlated traits were mapped in the same genomic regions forming QTL clusters. The largest QTL clusters, consisting of up to nine QTLs, were found on chromosomes 1D and 4D. HMW glutenin subunits at Glu-1 loci had the largest effect on breadmaking quality; however, other genomic regions also contributed genetically to breadmaking quality. QTLs detected in the present study are compared with other QTL analyses in wheat.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • American Association of Cereal Chemists (2000) Approved Methods of the AACC, 10th edn St. Paul. Methods 26-95A, 39-25, and 56-70

  • Araki E, Miura H, Sawada S (1999) Identification of genetic loci affecting amylose content and agronomic traits on chromosome 4A of wheat. Theor Appl Genet 98:977–984

    Article  CAS  Google Scholar 

  • Basten CJ, Weir BS, Zeng Z-B (2001) QTL Cartographer, version 1.15. Department of Statistics, North Carolina State University, Raleigh

  • Blanco A, Bellomo MP, Lotti C, Maniglio T, Pasqualone A, Simeone R, Troccoli A, Di Fonzo N (1998). Genetic mapping of sedimentation volume across environments using recombinant inbred lines of durum wheat. Plant Breed 117:413–417

    Article  Google Scholar 

  • Blanco A, Pasqualone A, Troccoli A, Di Fonzo N, Simeone R (2002) Detection of grain protein content QTLs across environments in tetraploid wheats. Plant Mol Biol 48:615–623

    Article  PubMed  CAS  Google Scholar 

  • Börner A, Schumann E, Fürste A, Cöster H, Leithold B, Röder MS, Weber WE (2002) Mapping of quantitative trait loci for agronomic important characters in hexaploid wheat (Triticum aestivum L.). Theor Appl Genet 105:921–936

    Article  PubMed  Google Scholar 

  • Cadalen T, Sourdille P, Charmet G, Tixier MH, Gay G, Boeuf C, Bernard S, Leroy P, Bernard M (1998) Molecular markers linked to genes affecting plant height in wheat using a doubled-haploid population. Theor Appl Genet 96:933–940

    Article  CAS  Google Scholar 

  • Campbell BT, Baenziger PS, Gill KS, Eskridge KM, Budak H, Erayman M, Dweikat I, Yen Y (2003) Identification of QTLs and environmental interactions associated with agronomic traits on chromosome 3A of wheat. Crop Sci 43:1493–1505

    Article  CAS  Google Scholar 

  • Chao S, Sharp PJ, Worland AJ, Warham EJ, Koebner RMD, Gale MD (1989) RFLP-based genetic maps of wheat homoeologous group 7 chromosomes. Theor Appl Genet 78:495–504

    Article  CAS  Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    PubMed  CAS  Google Scholar 

  • Doerge RW, Churchill GA (1996) Permutatuion tests for multiple loci affecting a quantitative character. Genetics 142:285–294

    PubMed  CAS  Google Scholar 

  • Elouafi I, Nachit MM (2004) A genetic linkage map of the Durum ×  Triticum dicoccoides backcross population based on SSRs and AFLP markers, and QTL analysis for milling traits. Theor Appl Genet 108:401–413

    Article  PubMed  CAS  Google Scholar 

  • Faris JD, Haen KM, Gill BS (2000) Saturation mapping of a gene-rich recombination hot spot region in wheat. Genetics 154:823–835

    PubMed  CAS  Google Scholar 

  • Gill KS, Gill BS, Endo TR, Taylor T (1996) Identification and high-density mapping of gene-rich regions in chromosome group 1 of wheat. Genetics 144:1883–1891

    PubMed  CAS  Google Scholar 

  • Groos C, Robert N, Bervas E, Charmet G (2003) Genetic analysis of grain protein-content, grain yield and thousand-kernel weight in bread wheat. Theor Appl Genet 106:1032–1040

    PubMed  CAS  Google Scholar 

  • Hsam SLK, Kieffer R, Zeller FJ (2001) Significance of Aegilops tauschii glutenin genes on breadmaking properties of wheat. Cereal Chem 78:521–525

    Article  CAS  Google Scholar 

  • Huang XQ, Röder MS (2004) Molecular mapping of powdery mildew resistance genes in wheat: a review. Euphytica 137:203–223

    Article  CAS  Google Scholar 

  • Huang XQ, Röder MS (2005) Development of SNP assays for genotyping of the puroindoline b gene for grain hardness in wheat using Pyrosequencing. J Agric Food Chem 53:2070–2075

    Article  PubMed  CAS  Google Scholar 

  • Huang XQ, Cöster H, Ganal MW, Röder MS (2003) Advanced backcross QTL analysis for the identification of quantitative trait loci alleles from wild relatives of wheat (Triticum aestivum L.). Theor Appl Genet 106:1379–1389

    PubMed  CAS  Google Scholar 

  • Huang XQ, Kempf H, Ganal MW, Röder MS (2004) Advanced backcross QTL analysis in progenies derived from a cross between a German elite winter wheat variety and a synthetic wheat (Triticum aestivum L.). Theor Appl Genet 109:933–943

    Article  PubMed  CAS  Google Scholar 

  • Joppa LR, Du C, Hart GE, Hareland GA (1997) Mapping gene(s) for grain protein in tetraploid wheat (Triticum turgidum L.) using a population of recombinant inbred chromosome lines. Crop Sci 37:1586–1589

    Article  CAS  Google Scholar 

  • Kato K, Miura H, Sawada S (2000) Mapping QTLs controlling grain yield and its components on chromosome 5A of wheat. Theor Appl Genet 101:1114–1121

    Article  CAS  Google Scholar 

  • Keller M, Karutz Ch, Schmid JE, Stamp P, Winzeler M, Keller B, Messmer MM (1999) Quantitative trait loci for lodging resistance in a segregating wheat × spelt population. Theor Appl Genet 98:1171–1182

    Article  CAS  Google Scholar 

  • Khan IA, Procunier JD, Humphreys DG, Tranquilli G, Schlatter AR, Marcucci-Poltri S, Frohberg R, Dubcovsky J (2000) Development of PCR-based markers for a high grain protein content gene from Triticum turgidum ssp. dicoccoides transferred to bread wheat. Crop Sci 40:518–524

    Article  CAS  Google Scholar 

  • Knapp SJ, Stroup WW, Ross WM (1985) Exact convidence intervals for heritability on a progeny mean basis. Crop Sci 25:192–194

    Article  Google Scholar 

  • Knox RE, De Pauw RM, McCaig TN, Clarke JM, McLeod JG, Fernandez MR (1995) AC Karma white spring wheat. Can J Plant Sci 75: 899–901

    Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Lukow OM, Forsyth SA, Payne PI (1992) Over-production of HMW glutenin subunits coded on chromosome 1B in common wheat, Triticum aestivum. J Genet Breed 46:187–192

    CAS  Google Scholar 

  • McCartney CA, Somers DJ, Humphreys DG, Lukow O, Ames N, Noll J, Cloutier S, McCallum BD (2005) Mapping quantitative trait loci controlling agronomic traits in the spring wheat cross RL4452 × ‘AC Domain’. Genome 48:870–883

    PubMed  CAS  Google Scholar 

  • McIntosh RA, Hart GE, Devos KM, Gale MD, Rogers WJ (1998) Catalogue of gene symbols for wheat. In: Slinkard AE (ed) Proceedings of the 9th International Wheat Genet Symp, vol 5. University Extension Press, University of Saskatchewan, Canada

  • Mesfin A, Frohberg RC, Anderson JA (1999) RFLP markers associated with high grain protein from Triticum turgidum L. var. dicoccoides introgressed into hard red spring wheat. Crop Sci 39:508–513

    Article  CAS  Google Scholar 

  • Payne PI (1987) Genetics of wheat strorage proteins and the effect of allelic variation on breadmaking quality. Annu Rev Plant Physiol 38:141–153

    Article  CAS  Google Scholar 

  • Peng JH, Ronin Y, Fahima T, Röder MS, Li YC, Nevo E, Korol A (2003) Domestication quantitative trait loci in Triticum dicoccoides, the progenitor of wheat. Proc Natl Acad Sci USA 100:2489–2494

    Article  PubMed  CAS  Google Scholar 

  • Pestsova E, Röder MS (2002) Microsatellite analysis of wheat chromosome 2D allows the reconstruction of chromosomal inheritance in pedigrees of breeding programmes. Theor Appl Genet 106:84–91

    PubMed  CAS  Google Scholar 

  • Pestsova E, Ganal MW, Röder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43:689–697

    Article  PubMed  CAS  Google Scholar 

  • Pon CR, Lukow OM, Buckley DJ (1989) A multichannel, computer-based system for analysing dough rheology. J Texture Stud 19:343–360

    Article  Google Scholar 

  • Prasad M, Kumar N, Kulwal PL, Röder MS, Balyan HS, Dhaliwal HS, Gupta PK (2003) QTL analysis for grain protein content using SSR markers and validation studies using NILs in bread wheat. Theor Appl Genet 106:659–667

    PubMed  CAS  Google Scholar 

  • Radovanovic N, Cloutier S (2003) Gene-assisted selection for high molecular weight glutenin subunits in wheat doubled haploid breeding programs. Mol Breed 12:51–59

    Article  CAS  Google Scholar 

  • Radovanovic N, Cloutier S, Brown D, Humphreys DG, Lukow OM (2002) Genetic variance for gluten strength contributed by high molecular weight glutenin proteins. Cereal Chem 79:843–849

    Article  CAS  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Röder MS, Huang XQ, Ganal MW (2004) Wheat microsatellites in plant breeding—potential and implications. In: Lörz H, Wenzel G (eds) Biotechnology in agriculture and forestry, vol 55, molecular marker systems in plant breeding and crop improvement. Springer, Berlin Heidelberg New York, pp 255–266

  • Sandhu D, Champoux JA, Bondareva SN, Gill KS (2001) Identification and physical localization of useful genes and markers to a major gene-rich region on wheat group 1S chromosomes. Genetics 157:1735–1747

    PubMed  CAS  Google Scholar 

  • Schuelke M. (2000) An economic method for the fluorescent labelling of PCR fragments. Nat Biotechnol 18:233–234

    Article  PubMed  CAS  Google Scholar 

  • Shah MM, Gill KS, Baenziger PS, Yen Y, Kaeppler SM, Ariyarathne HM (1999) Molecular mapping of loci for agronomic traits on chromosome 3A of bread wheat. Crop Sci 39:1728–1732

    Article  CAS  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  PubMed  CAS  Google Scholar 

  • Sourdille P, Singh S, Cadalen T, Brown-Guedira GL, Gay G, Qi L, Gill BS, Dufour P, Murigneux A, Bernard M (2004) Microsatellite-based deletion bin system for the establishment of genetic–physical map relationships in wheat (Triticum aestivum L.). Funct Integr Genomics 4:12–25

    Article  PubMed  CAS  Google Scholar 

  • Wang S, Basten CJ, Zeng Z-B (2004) Windows QTL Cartographer 2.0. Department of Statistics, North Carolina State University, Raleigh (http://www.statgen.ncsu.edu/qtlcart/WQTLCart.htm)

  • Zeng Z-B (1994) Precision mapping of quantitative trait loci. Genetics 136:1457–1468

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We would like to thank the breeding teams at Winnipeg and Brandon for the field experiments and evaluations, Elsa Reimer for excellent assistance in genotyping and Sheila Woods for statistical analysis advice. Review of the manuscript and useful comments provided by Andrzej Walichnowski, and Drs Curt McCartney and David De Koeyer are greatly acknowledged. X. Q. Huang received a visiting fellowship from the National Science and Engineering Research Council of Canada (NSERC). This research was supported in part by an NSERC post-graduate scholarship to N. Radovanovic. This publication is Agriculture and Agri-Food Canada contribution no. 1921.

Author information

Authors and Affiliations

  1. Cereal Research Centre, Agriculture and Agri-Food Canada, 195 Dafoe Road, R3T 2M9, MB, Winnipeg, Canada

    X. Q. Huang, S. Cloutier, L. Lycar, N. Radovanovic, D. G. Humphreys, J. S. Noll, D. J. Somers & P. D. Brown

Authors
  1. X. Q. Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Cloutier
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. Lycar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. Radovanovic
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. D. G. Humphreys
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. J. S. Noll
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. D. J. Somers
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. P. D. Brown
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. Cloutier.

Additional information

Communicated by M. Bohn.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Huang, X.Q., Cloutier, S., Lycar, L. et al. Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats (Triticum aestivum L.). Theor Appl Genet 113, 753–766 (2006). https://doi.org/10.1007/s00122-006-0346-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-006-0346-7

Keywords

Search

Navigation