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Advanced backcross QTL analysis in barley (Hordeum vulgare L.)

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Abstract.

This paper reports on the first advanced backcross-QTL (quantitative trait locus) project which utilizes spring barley as a model. A BC2F2 population was derived from the initial cross Apex (Hordeum vulgare ssp. vulgare, hereafter abbreviated with Hv) × ISR101-23 (H. v. ssp. spontaneum, hereafter abbreviated with Hsp). Altogether 136 BC2F2 individuals were genotyped with 45 SSR (simple sequence repeat) markers. Subsequently, field data for 136 BC2F2 families were collected for 13 quantitative traits measured in a maximum of six environments. QTLs were detected by means of a two-factorial ANOVA with a significance level of P < 0.01 for a marker main effect and a marker × environment (M × E) interaction, respectively. Among 585 marker × trait combinations tested, 86 putative QTLs were identified. At 64 putative QTLs, the marker main effect and at 27 putative QTLs, the M × E interaction were significant. In five cases, both effects were significant. Among the putative QTLs, 29 (34%) favorable effects were identified from the exotic parent. At these marker loci the homozygous Hsp genotype was associated with an improvement of the trait compared to the homozygous Hv genotype. In one case, the Hsp allele was associated with a yield increase of 7.7% averaged across the six environments tested. A yield QTL in the same chromosomal region was already reported in earlier barley QTL studies.

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Acknowledgements.

We wish to thank Dr. Eberhard Laubach and Dr. Volker Lein and their teams for conducting field experiments and collecting trait data at the breeding companies Nordsaat, Gudow, and Ackermann, Irlbach. We also thank Dr. Lizzy Kuntze and her team for measuring quality traits at the laboratory of Nordsaat, Böhnhausen. In addition, the excellent technical assistance of Karola Müller and the technical team of our experimental station Dikopshof at Wesseling is appreciated. We are indebted to Prof. G. Fischbeck, Technical University, München, for providing the wild barley accession ISR101-23. We thank Mrs. Maria von Korff for critical reading of the manuscript. This work was supported by grants Pi339/1-1 and Pi339/1-2 from the Deutsche Forschungsgemeinschaft (DFG).

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Authors and Affiliations

  1. Department of Crop Science and Plant Breeding, University of Bonn, Katzenburgweg 5, 53115 Bonn, Germany

    K. Pillen & J. Léon

  2. KWS, Kleinwanzlebener Saatzucht AG, Grimsehlstr. 31, 37555 Einbeck, Germany

    A. Zacharias

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  1. K. Pillen
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  2. A. Zacharias
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  3. J. Léon
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Correspondence to K. Pillen.

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Communicated by G. Wenzel

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Pillen, K., Zacharias, A. & Léon, J. Advanced backcross QTL analysis in barley (Hordeum vulgare L.). Theor Appl Genet 107, 340–352 (2003). https://doi.org/10.1007/s00122-003-1253-9

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  • DOI: https://doi.org/10.1007/s00122-003-1253-9

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