Proteomics Perspectives in Post-Genomic Era for Producing Salinity Stress-Tolerant Crops

  • Pannaga KrishnamurthyEmail author
  • Lin Qingsong
  • Prakash P. Kumar


Plant growth and productivity are affected by both biotic and abiotic stress factors. Among the abiotic stresses, salt stress is the most prevalent and deleterious environmental factor which limits crop yield globally. Combined with the increasing population and food demands, this poses a great challenge to humanity. Currently, salinity affects more than 20% of the irrigated land. This is estimated to increase drastically in the near future due to the excessive irrigation practices. These factors have necessitated the researchers to understand the salt tolerance mechanisms in plants in order to use various approaches to generate salt-tolerant crops. Due to their sessile nature, plants cannot evade the stressful environment, and therefore, some species have evolved various adaptive strategies to grow and reproduce under unfavorable environments. Salt stress imparts both osmotic and ionic stress to the plants, affecting their metabolism and ion homeostasis, thereby leading to reduced growth and productivity and death in some cases. Salt tolerance is a complex phenomenon involving changes in the biochemical, molecular, and physiological processes of the plant. These changes consisting of a readjustment in the genomic and proteomic complement of the plants are imperative in unraveling the tolerance mechanisms. Recent advances in the omics research have shed more light on a range of promising candidate genes and proteins that render salt tolerance to plants. In this chapter, we describe the general effects of salt stress, the tolerance mechanisms of plants, and how recent advances in the field of proteomics can be utilized to enhance salt tolerance of crop plants.


Proteomics Salt stress Salt tolerance Crop plants 



One-dimensional gel electrophoresis


Two-dimensional gel electrophoresis


Abscisic acid


Ascorbate peroxidase


Basic helix-loop-helix




CoA O-methyltransferase


Cyclic nucleotide-gated channels


Dehydroascorbate reductase


Difference gel electrophoresis


Electric conductivity


Glutathione peroxidase


High-affinity potassium transporter


Isotope-coded affinity tags


Isobaric tags for relative and absolute quantitation


Jasmonic acid


Leucine-rich repeat


Mitogen-activated protein kinase


Monodehydroascorbate reductase


Multiple reaction monitoring


Mass spectrometry


Multidimensional protein identification technology


Sodium chloride


Sodium/hydrogen exchanger


Nonselective cation channels


Plasma membrane intrinsic proteins




Posttranslational modifications


Reactive oxygen species


Salicylic acid


S-adenosyl methionine


Stable isotope labeling by amino acids in cell culture


Superoxide dismutase


Salt overly sensitive1


Selective reaction monitoring


Small ubiquitin-like modifiers


Sequential window acquisition of all theoretical mass spectra


Tonoplast intrinsic proteins


Voltage-dependent anion channel


Vacuolar pyrophosphatase



The research work in our laboratory is supported by the Singapore National Research Foundation under its Environment and Water Research Programme and administered by PUB, Singapore’s National Water Agency, Singapore, NRF-EWI-IRIS (R-706-000-010-272 and R-706-000-040-279).


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Pannaga Krishnamurthy
    • 1
    Email author
  • Lin Qingsong
    • 1
  • Prakash P. Kumar
    • 1
  1. 1.Department of Biological Sciences, and NUS Environmental Research Institute (NERI)National University of SingaporeSingaporeSingapore

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