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The proteome response of salt-resistant and salt-sensitive barley genotypes to long-term salinity stress

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Abstract

Responses of plants to salinity stress and the development of salt tolerance are extremely complex. Proteomics is a powerful technique to identify proteins associated with a particular environmental or developmental signal. We employed a proteomic approach to further understand the mechanism of plant responses to salinity in a salt-tolerant (Afzal) and a salt-sensitive (Line 527) genotype of barley. At the 4-leaf stage, plants were exposed to 0 (control) or 300 mM NaCl. Salt treatment was maintained for 3 weeks. Total proteins of leaf 4 were extracted and separated by two-dimensional gel electrophoresis. More than 500 protein spots were reproducibly detected. Of these, 44 spots showed significant changes to salt treatment compared to the control: 43 spots were upregulated and 1 spot was downregulated. Using MALDI-TOF-TOF MS, we identified 44 cellular proteins have been identified, which represented 18 different proteins and were classified into seven categories and a group with unknown biological function. These proteins were involved in various many cellular functions. Up regulation of proteins which involved in reactive oxygen species scavenging, signal transduction, protein processing and cell wall may increase plant adaptation to salt stress. The upregulation of the three of four antioxidant proteins (thioredoxin, methionine sulfoxide reductase and dehydroascorbate reductase) in susceptible genotype Line 527 suggesting a different tolerance mechanism (such as tissue tolerance) to tolerate a salinity condition in comparison with the salt sensitive genotype.

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Acknowledgments

We thank Dr Kazem Poustini for assistance with the glasshouse experiment. This work was financially supported by the Center of Excellence of Agronomy, Breeding and Biotechnology of Forage Crops at the University of Tehran.

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Authors and Affiliations

  1. Department of Agronomy and Plant Breeding, University of Tehran, Karaj, Iran

    Foad Fatehi, Abdolhadi Hosseinzadeh & Houshang Alizadeh

  2. Department of Food Science and Technology, Faculty of Agriculture, Islamic Azad University of Shariar (Shahr-e-Ghods) Beranch, Tehran, Iran

    Tahereh Brimavandi

  3. Centre for Crop Systems Analysis, Plant Sciences Group, Wageningen University, Droevendaalsesteeg, 6708 PB, Wageningen, The Netherlands

    Paul C. Struik

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  1. Foad Fatehi
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Fatehi, F., Hosseinzadeh, A., Alizadeh, H. et al. The proteome response of salt-resistant and salt-sensitive barley genotypes to long-term salinity stress. Mol Biol Rep 39, 6387–6397 (2012). https://doi.org/10.1007/s11033-012-1460-z

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