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Effects of NaCl and iso-osmotic PEG stress on growth, osmolytes accumulation and antioxidant defense in cultured sugarcane cells

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Abstract

In order to discriminate between the ionic and osmotic components of salt stress, sugarcane (Saccharum officinarum L. cv. Co 86032) calli were cultured on media containing NaCl or polyethylene glycol (PEG) 8000 that exerted the same osmotic pressure (−0.7 MPa). PEG stress exposure for 15 days led to significant growth reduction and loss in water content than salt stressed and control tissues. Osmotic adjustment (OA) was observed in callus tissues grown on salt, but was not evident in callus grown on PEG. Oxidative damage to membranes, estimated in terms of accumulation of thiobarbituric acid reactive substances-TBARS and electrolytic leakage was significantly higher in both the stressed calli than the control however, the extent of damage was more in the PEG stressed calli. The stressed callus tissues showed inhibition of ascorbate peroxidase activity, while catalase activity was increased. These results indicate sensitivity of cells to PEG-mediated stress than salt stress and differences in their OA to these two stress conditions. The sensitivity to the osmotic stress indicate that expression of the stress tolerance response requires the coordinated action of different tissues in a plant and hence was not expressed at the cellular level.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxy acetic acid

APX:

Ascorbate peroxidase (EC 1.11.1.11)

CAT:

Catalase (EC 1.11.1.6)

DNSA:

Dinitro salicylic acid

EDTA:

Ethylene diamine tetra acetic acid

GB:

Glycine betaine

KI-I2 :

Potassium iodide-iodine reagent

MD:

Membrane damage

MS:

Murashige and Skoog basal medium

NBT:

Nitro blue tetrazolium

OA:

Osmotic adjustment

PFD:

Photon flux density

PVP:

Polyvinyl pyrrolidone

RGR:

Relative growth rate

RH:

Relative humidity

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase (EC 1.15.1.1)

TBA:

Thiobarbituric acid

TBARS:

Thiobarbituric acid reactive substances

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Acknowledgments

The senior author is grateful to the University Grant Commission, New Delhi and Botany Department, University of Pune, Pune for award of a UGC Research fellowship.

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Author notes

  1. Vikas Yadav Patade

    Present address: Molecular Biology and Genetic Engineering Division, Defence Institute of Bio-Energy Research, Goraparao, Haldwani, Nainital, 263139, India

Authors and Affiliations

  1. Functional Plant Biology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India

    Vikas Yadav Patade & Penna Suprasanna

  2. Botany Department, University of Pune, Pune, 411007, India

    Vikas Yadav Patade & Sujata Bhargava

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  1. Vikas Yadav Patade
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Correspondence to Penna Suprasanna.

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Patade, V.Y., Bhargava, S. & Suprasanna, P. Effects of NaCl and iso-osmotic PEG stress on growth, osmolytes accumulation and antioxidant defense in cultured sugarcane cells. Plant Cell Tiss Organ Cult 108, 279–286 (2012). https://doi.org/10.1007/s11240-011-0041-5

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