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Effects of NaCl and mannitol induced stress on sugarcane (Saccharum sp.) callus cultures

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Abstract

The effects of NaCl and mannitol iso-osmotic stresses on calli issued from sugarcane cultivars (cvs.) R570, CP59-73 and NCo310 were investigated in relation to callus growth, water content, ion and proline concentrations. Callus growth and water content decreased under both stresses with the highest reduction under mannitol-induced osmotic stress. The ion concentration was drastically affected after exposure to NaCl and mannitol. Salt stress induced an increase in Na+ and Cl− accumulation and a decrease in K+ and Ca2+ concentrations. Under mannitol-induced osmotic stress, K+ and Ca2+ concentrations decreased significantly while Na+ and Cl− concentrations remained unchanged. Free proline accumulation occurred under both stresses and was more marked in stress-sensitive cv. than in stress-resistant one. Our results indicated that the physiological mechanisms operating at the plant cell level in response to salt- and osmotic-induced stress in sugarcane cvs. are different. Among the cvs., we concluded that the stress resistance is closely related to the maintain of an adequate water status and a high level of K+ and Ca2+ under both stresses and a low level of Na+ concentration in the presence of NaCl. Thus, sugarcane (Saccharum sp.) can be regarded as a Na+ excluder. We also provided evidence that proline accumulation is a stress-sensitive trait rather than a stress resistance marker.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

cv(s):

Cultivars

MS:

Murashige and Skoog

RGR:

Relative growth rate

ANOVA:

Analyses of variance

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Acknowledgments

The authors are very grateful to D. El Ghrassli (Centre Technique des Cultures Sucrières, Morocco) for kindly providing the sugarcane cultivars. This research was financially supported by funds of Programme d’Appui à la Recherche Scientifique (PARS AGRO 180) from the Ministère de l’Enseignement Supérieur, de la Formation des Cadres et de la Recherche Scientifique of Morocco.

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Authors and Affiliations

  1. Laboratoire de Biologie et Santé, Equipe de Biotechnologies et Microbiologie Appliquée, Université Abdelmalek Essaâdi, Faculté des Sciences, BP 2121, Tétouan, Morocco

    Tomader Errabii, Christophe Bernard Gandonou, Jamal Abrini, Mohamed Idaomar & Nadia Skali Senhaji

  2. Laboratoire de Biotechnologie Végétale, Université Abdelmalek Essaâdi, Faculté des Sciences et Techniques, BP 416, Tanger, Morocco

    Hayat Essalmani

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  1. Tomader Errabii
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  2. Christophe Bernard Gandonou
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  3. Hayat Essalmani
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  4. Jamal Abrini
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  5. Mohamed Idaomar
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  6. Nadia Skali Senhaji
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Correspondence to Nadia Skali Senhaji.

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Communicated by M. Filek.

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Errabii, T., Gandonou, C.B., Essalmani, H. et al. Effects of NaCl and mannitol induced stress on sugarcane (Saccharum sp.) callus cultures. Acta Physiol Plant 29, 95–102 (2007). https://doi.org/10.1007/s11738-006-0006-1

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  • DOI: https://doi.org/10.1007/s11738-006-0006-1

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