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Drought Induces Oxidative Stress and Enhances the Activities of Antioxidant Enzymes in Growing Rice Seedlings

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Abstract

When rice seedlings grown for 10 and 20 days were subjected to in vitro drought stress of −0.5 and −2.0 MPa for 24 h, an increase in the concentration of superoxide anion (O2.−), increased level of lipid peroxidation and a decrease in the concentration of total soluble protein and thiols was observed in stressed seedlings compared to controls. The concentration of H2O2 as well as ascorbic acid declined with imposition of drought stress, however glutathione (GSH) concentration declined only under severe drought stress. The activities of total superoxide dismutases (SODs) as well as ascorbate peroxidase (APX) showed consistent increases with increasing levels of drought stress, however catalase activity declined. Mild drought stressed plants had higher guaiacol peroxidase (GPX) and chloroplastic ascorbate peroxidase (c-APX) activity than control grown plants but the activity declined at the higher level of drought stress. The activities of enzymes involved in regeneration of ascorbate i.e. monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR) and glutathione reductase (GR) were higher in drought stressed plants compared to controls. Results suggest that drought stress induces oxidative stress in rice plants and that besides SOD, the enzymes of ascorbate-glutathione cycle, which have not been studied in detail earlier under stressful conditions, appear to function as important component of antioxidative defense system under drought stress.

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Abbreviations

AsA :

ascorbate

CAT:

catalase

c-APX:

chloroplastic ascorbate peroxidase

DHA:

dehydroascorbate

DHAR:

dehydroascorbate reductase

GPX:

guaiacol peroxidase

GR:

glutathione reductase

GSH:

glutathione

GSSG:

oxidized glutathione

MDHAR:

monodehydroascorbate reductase

SOD:

superoxide dismutase

TBARS:

thiobarbituric acid reactive substances

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Banaras Hindu University, 221005, Varanasi, India

    Pallavi Sharma & Rama Shanker Dubey

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  1. Pallavi Sharma
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  2. Rama Shanker Dubey
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Correspondence to Rama Shanker Dubey.

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Sharma, P., Dubey, R.S. Drought Induces Oxidative Stress and Enhances the Activities of Antioxidant Enzymes in Growing Rice Seedlings. Plant Growth Regul 46, 209–221 (2005). https://doi.org/10.1007/s10725-005-0002-2

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  • DOI: https://doi.org/10.1007/s10725-005-0002-2

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