Abstract
In vitro responses of embryogenic sugarcane (Saccharum officinarum L.; cv. CoC-671) calli stressed with different levels of NaCl (0.0, 42.8, 85.6, 128.3, 171.1, 213.9 or 256.7 mM) were studied. The results showed that a significant decrease in callus growth and cell viability occurred with ≥85.6 mM NaCl. Higher amounts of free proline and glycine betaine were accumulated in NaCl-stressed calli. Although the leached and retained Na+ contents increased, the retained K+ content decreased with increasing levels of NaCl. Such a mechanism implies that sugarcane can be considered as a Na+-excluder. The accumulation of salt ions and osmolytes could play an important role in osmotic adjustment in sugarcane cells under salt stress.
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Abbreviations
- 2,4-D:
-
2,4-Dichlorophenoxyacetic acid
- FW:
-
Fresh weight
- MS:
-
Murashige and Skoog’s medium
- KI–I2 :
-
Potassium iodide–iodine reagent
- RGR:
-
Relative growth rate
- TTC:
-
2,3,5-Triphenyltetrazolium chloride
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Acknowledgments
We thank Dr. S.N. Jamdar for his help and guidance in biochemical estimations and Dr. Anand Badigannawar for statistical analysis. PVY is grateful to ASPEE Agricultural Research and Development Foundation, Mumbai, India for a research fellowship.
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Patade, V.Y., Suprasanna, P. & Bapat, V.A. Effects of salt stress in relation to osmotic adjustment on sugarcane (Saccharum officinarum L.) callus cultures. Plant Growth Regul 55, 169–173 (2008). https://doi.org/10.1007/s10725-008-9270-y
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DOI: https://doi.org/10.1007/s10725-008-9270-y