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Hydrogen peroxide and nitric oxide mediate K+/Na+ homeostasis and antioxidant defense in NaCl-stressed callus cells of two contrasting poplars

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Abstract

Using callus cells of a salt-tolerant Populus euphratica Oliver and a salt-sensitive P. popularis 35–44 (P. popularis), the effects of NaCl stress on hydrogen peroxide (H2O2) and nitric oxide (NO) production and the relevance to ionic homeostasis and antioxidant defense were investigated. Results show that P. euphratica exhibited a greater capacity to tolerate NaCl stress in terms of cell viability, membrane permeability and K+/Na+ relations. NaCl salinity (150 mM) caused a rapid increase of H2O2 and NO in P. euphratica cells, but not in P. popularis. Moreover, salinised P. euphratica cells retained a high and stable level of H2O2 and NO during the period of 24-h salt stress. Noteworthy, P. eupratica cells increased activities of superoxide dismutase, ascorbate peroxidase, catalase and glutathione reductase under salinity stress, but these antioxidant enzymes were significantly inhibited by the salt treatment in P. popularis cells. Pharmacological experiments proved that the NaCl-induced H2O2 and NO was interdependent and contributed to the mediation of K+/Na+ homeostasis and antioxidant defense in P. euphratica cells. Given these results, we conclude that the increased H2O2 and NO enable P. euphratica cells to regulate ionic and ROS (reactive oxygen species) homeostasis under salinity stress in the longer term.

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Acknowledgments

This research was supported by the project of Scientific and Technical Innovation for the Graduate Student, Beijing Forestry University (BLYJ200903). We acknowledge the offer for the use of the confocal miscroscope and scanning electron microscope by the Platform of Large Instruments and Equipment at Beijing Forestry University. We thank Ms Hui Zhang for her contribution to the EDAX analysis.

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Authors and Affiliations

  1. National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Box 162, Beijing, 100083, People’s Republic of China

    Jian Sun, Lisi Li, Meiqin Liu, Meijuan Wang, Mingquan Ding, Shurong Deng, Cunfu Lu, Xiaoyang Zhou, Xin Shen & Shaoliang Chen

  2. Key Laboratory of Biological Resources Protection and Utilization in Hubei Province, Hubei University for Nationalities, Enshi, 445000, People’s Republic of China

    Xiaojiang Zheng & Shaoliang Chen

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  1. Jian Sun
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  2. Lisi Li
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Correspondence to Shaoliang Chen.

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The Authors Jian Sun and Lisi Li contributed equally to this work.

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Sun, J., Li, L., Liu, M. et al. Hydrogen peroxide and nitric oxide mediate K+/Na+ homeostasis and antioxidant defense in NaCl-stressed callus cells of two contrasting poplars. Plant Cell Tiss Organ Cult 103, 205–215 (2010). https://doi.org/10.1007/s11240-010-9768-7

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  • DOI: https://doi.org/10.1007/s11240-010-9768-7

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