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Cold-shock gene cspC in the genome of Massilia polaris sp. nov. revealed cold-adaptation

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Abstract

A straw coloured, motile and Gram-stain-negative bacterium, designated RP-1-19T was isolated from soil of Arctic station, Svalbard, Norway. Based on the phylogenetic analysis of its 16S rRNA gene sequence, strain RP-1-19T formed a lineage within the family Oxalobacteraceae and clustered together within the genus Massilia. The closest members were M. violaceinigra B2T (98.6% sequence similarity), M. eurypsychrophilia JCM 30074T (98.3%) and M. atriviolacea SODT (98.1%). The only respiratory quinone was ubiquinone-8. The principal cellular fatty acids were summed feature 3 (iso-C15:0 2-OH/C16:1ω7c) and C16:0. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The DNA G + C content of the type strain was 63.2%. The average nucleotide identity and in silico DNA–DNA hybridization values between strain RP-1-19T and closest members were ≤ 80 and 23.2%, respectively. The genome was 4,522,469 bp long with 30 scaffolds and 4076 protein-coding genes. The genome showed eight putative biosynthetic gene clusters responsible for various secondary metabolites. Genome analysis revealed the presence of cold-shock proteins CspA and CspC. Presence of cspA and cspC genes in the genome manifest ecophysiology of strain RP-1-19T that may help in cold-adaptation. Based on these data, strain RP-1-19T represents a novel species in the genus Massilia, for which the name Massilia polaris sp. nov. is proposed. The type strain is RP-1-19T (= KACC 21619T = NBRC 114359T).

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Acknowledgements

We thank Prof. Aharon Oren (The Hebrew University of Jerusalem, Israel) for his expert suggestions concerning the correct species epithet and etymology.

Funding

This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. 2019R1F1A1058501).

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Authors and Affiliations

  1. Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Kyonggi-Do, 16227, Republic of Korea

    Ram Hari Dahal & Jaisoo Kim

  2. Department of Microbiology, School of Medicine, Kyungpook National University, Daegu, 41944, Republic of Korea

    Ram Hari Dahal

  3. Department of Environmental Engineering, Korea University Sejong Campus, Sejong City, 30019, Republic of Korea

    Dhiraj Kumar Chaudhary

  4. Department of Biological Science, College of Science and Engineering, Sangji University, Wonju, Republic of Korea

    Dong-Uk Kim

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  1. Ram Hari Dahal
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  2. Dhiraj Kumar Chaudhary
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  4. Jaisoo Kim
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Correspondence to Jaisoo Kim.

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Dahal, R.H., Chaudhary, D.K., Kim, DU. et al. Cold-shock gene cspC in the genome of Massilia polaris sp. nov. revealed cold-adaptation. Antonie van Leeuwenhoek 114, 1275–1284 (2021). https://doi.org/10.1007/s10482-021-01600-z

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