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Arthrobacter terrae sp. nov., a psychrophilic actinobacterium with multi copies of capA gene isolated from Antarctic soil

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Abstract

A Gram-staining-positive, non-spore-forming, non-flagellated, ellipsoidal, strain Z1-20 T belonging to the genus Arthrobacter was isolated from a soil sample collected from the Zhongshan station, Antarctic. Phylogenetic analysis of the 16S rRNA gene sequences and phylogenetic analysis revealed that strain Z1-20 T formed a unique single cluster in the genus Arthrobacter and shared high 16S rRNA sequence similarities of 97.1% and 96.9% with A. glacialis HLT2-12-2 T and A. psychrochitiniphilus GP3T, respectively. Values of Digital DNA-DNA hybridization (dDDH) between strain Z1-20 T against A. glacialis HLT2-12-2 T and A. psychrochitiniphilus GP3T were 20.3% and 13.8%, respectively. Average nucleotide identity (ANI) score between strain Z1-20 T against A. glacialis HLT2-12-2 T and A. psychrochitiniphilus GP3T were 72.5% and 72.1%, respectively. Genes for the synthesis of the osmoprotectant glycine betaine and higher copies of capA gene encoding cold shock protein were found in genome of Z1-20 T that may help Z1-20 T in cold-adaptation. Strain Z1-20 T comprised lysine as the diagnostic diamino acid. Based on the results of phylogenetic, phenotypic and chemotaxonomic features, strain Z1-20 T represents a novel species of a novel taxon of genus Arthrobacter, for which the name Arthrobacter terrae gen. nov., sp. nov. is proposed.

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Data availability

The genome sequence of Arthrobacter terrae Z1-20 T has been deposited in the GenBank database under the accession number JADNYM000000000. The GenBank accession number of the 16S rRNA gene sequence of strain Z1-20 T is MN334158.

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Acknowledgements

This work was supported by the National Key R&D Program of China (grants 2018YFC1406705).

Funding

The National Key R& D Program of China, 2018YFC1406705, Jing Li.

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Authors and Affiliations

  1. College of Marine Life Sciences, Ocean University of China, Qingdao, Shandong, 266003, People’s Republic of China

    Peiqiang Jiang, Xintao Ren, Wenjing Wang, Guojiang Niu & Jing Li

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  1. Peiqiang Jiang
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  2. Xintao Ren
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PJ, XR, WW and GN equally contributed to this work.

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Correspondence to Jing Li.

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Jiang, P., Ren, X., Wang, W. et al. Arthrobacter terrae sp. nov., a psychrophilic actinobacterium with multi copies of capA gene isolated from Antarctic soil. Antonie van Leeuwenhoek 115, 635–644 (2022). https://doi.org/10.1007/s10482-022-01727-7

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