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An integrative genetic linkage map of winter wheat (Triticum aestivum L.)

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Abstract

We constructed a genetic linkage map based on a cross between two Swiss winter wheat (Triticum aestivum L.) varieties, Arina and Forno. Two-hundred and forty F5 single-seed descent (SSD)-derived lines were analysed with 112 restriction fragment length polymorphism (RFLP) anonymous probes, 18 wheat cDNA clones coding for putative stress or defence-related proteins and 179 simple-sequence repeat (SSR) primer-pairs. The 309 markers revealed 396 segregating loci. Linkage analysis defined 27 linkage groups that could all be assigned to chromosomes or chromosome arms. The resulting genetic map comprises 380 loci and spans 3,086 cM with 1,131 cM for the A genome, 920 cM for the B genome and 1,036 cM for the D genome. Seventeen percent of the loci showed a significant (P < 0.05) deviation from a 1:1 ratio, most of them in favour of the Arina alleles. This map enabled the mapping of QTLs for resistance against several fungal diseases such as Stagonospora glume blotch, leaf rust and Fusarium head blight. It will also be very useful for wheat genetic mapping, as it combines RFLP and SSR markers that were previously located on separate maps.

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References

  • Bryan GJ, Collins AJ, Stephenson P, Orry A, Smith JB, Gale MD (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat. Theor Appl Genet 94:557–563

    Article  CAS  Google Scholar 

  • Cadalen T, Boeuf C, Bernard S, Bernard M (1997) An intervarietal molecular marker map in Triticum aestivum L em Thell and comparison with a map from a wide cross. Theor Appl Genet 94:367–377

    Article  CAS  Google Scholar 

  • Campbell KG, Bergman CJ, Gualberto DG, Anderson JA, Giroux MJ, Hareland G, Fulcher RG, Sorrells ME, Finney PL (1999) Quantitative trait loci associated with kernel traits in a soft × hard wheat cross. Crop Sci 39:1184–1195

    CAS  Google Scholar 

  • Chalmers KJ, Campbell AW, Kretschmer J, Karakousis A, Henschke PH, Pierens S, Harker N, Pallotta M, Cornish GB, Shariflou MR, Rampling LR, McLauchlan A, Daggard G, Sharp PJ, Holton TA, Sutherland MW, Appels R, Langridge P (2001) Construction of three linkage maps in bread wheat, Triticum aestivum. Aust J Agric Res 52:1089–1119

    Article  CAS  Google Scholar 

  • Chao S, Sharp PJ, Worland AJ, Warham EJ, Koebner RMD, Gale MD (1989) RFLP–based genetic maps of wheat homoeologous group-7 chromosomes. Theor Appl Genet 78:495–504

    CAS  Google Scholar 

  • Clough SJ, Fengler KA, Yu IC, Lippok B, Smith RK, Bent AF (2000) The Arabidopsis dnd1 "defense, no death" gene encodes a mutated cyclic nucleotide-gated ion channel. Proc Natl Acad Sci USA 97:9323–9328

    Article  CAS  PubMed  Google Scholar 

  • Devey ME, Hart GE (1993) Chromosomal localization of intergenomic RFLP loci in hexaploid wheat. Genome 36:913–918

    CAS  Google Scholar 

  • Feuillet C, Schachermayr G, Keller B (1997) Molecular cloning of a new receptor-like kinase gene encoded at the Lr10 disease resistance locus of wheat. Plant J 11:45–52

    CAS  PubMed  Google Scholar 

  • Gale MD, Atkinson MD, Chinoy CN, Harcourt RL, Liu J, Li QY, DK M (1995) Genetic maps of hexaploid wheat. In: Li ZS, Xin ZY (eds) Proc 8th Int Wheat Genet Symp, Agric Scientech Press, Beijing, pp 29–40

  • Gill KS, Lubbers EL, Gill BS, Raupp WJ, Cox TS (1991) A genetic-linkage map of Triticum tauschii (DD) and its relationship to the D-genome of bread wheat (AABBDD). Genome 34:362–374

    Google Scholar 

  • Gorlach J, Volrath S, KnaufBeiter G, Hengy G, Beckhove U, Kogel KH, Oostendorp M, Staub T, Ward E, Kessmann H, Ryals J (1996) Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat. Plant Cell 8:629-643

    CAS  PubMed  Google Scholar 

  • Graner A, Jahoor A, Schondelmaier J, Siedler H, Pillen K, Fischbeck G, Wenzel G, Herrmann RG (1991) Construction of an RFLP map of barley. Theor Appl Genet 83:250–256

    Google Scholar 

  • Groos C, Gay G, Perretant MR, Gervais L, Bernard M, Dedryver F, Charmet D (2002) Study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a white × red grain bread-wheat cross. Theor Appl Genet 104:39–47

    CAS  Google Scholar 

  • Gupta PK, Balyan HS, Edwards KJ, Isaac P, Korzun V, Röder M, Gautier MF, Joudrier P, Schlatter AR, Dubcovsky J, De la Pena RC, Khairallah M, Penner G, Hayden MJ, Sharp P, Keller B, Wang RCC, Hardouin JP, Jack P, Leroy P (2002) Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theor Appl Genet 105:413–422

    Article  Google Scholar 

  • Guyomarc'h H, Sourdille P, Edwards KJ, Bernard M (2002) Studies of the transferability of microsatellites derived from Triticum tauschii to hexaploid wheat and to diploid related species using amplification, hybridization and sequence comparisons. Theor Appl Genet 105:736–744

    Article  Google Scholar 

  • Haldane JBS (1919) The combination of linkage values, and the calculation of distance between loci of linked factors. J Genet 8:299–309

    Google Scholar 

  • Harding SA, Oh SH, Roberts DM (1997) Transgenic tobacco expressing a foreign calmodulin gene shows an enhanced production of active oxygen species. EMBO J 16:1137–1144

    Article  CAS  PubMed  Google Scholar 

  • Heun M, Kennedy AE, Anderson JA, Lapitan NLV, Sorrells ME, Tanksley SD (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare). Genome 34:437–447

    Google Scholar 

  • Hirschi KD (1999) Expression of Arabidopsis CAX1 in tobacco: altered calcium homeostasis and increased stress sensitivity. Plant Cell 11:2113–2122

    Article  CAS  PubMed  Google Scholar 

  • Jenczewski E, Gherardi M, Bonnin I, Prosperi JM, Olivieri I, Huguet T (1997) Insight on segregation distortions in two intraspecific crosses between annual species of Medicago (Leguminosae). Theor Appl Genet 94:682–691

    Google Scholar 

  • Kammholz SJ, Campbell AW, Sutherland MW, Hollamby GJ, Martin PJ, Eastwood RF, Barclay I, Wilson RE, Brennan PS, Sheppard JA (2001) Establishment and characterisation of wheat genetic mapping populations. Aust J Agric Res 52:1079–1088

    CAS  Google Scholar 

  • Lagudah ES, Appels R, Brown AHD, McNeil D (1991) The molecular-genetic analysis of Triticum tauschii, the D-genome donor to hexaploid wheat. Genome 34:375–386

    CAS  Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    CAS  PubMed  Google Scholar 

  • Leroy P (1995) Le catalogue des clones Génoblé. Publisher: Ministére de l'Agriculture, INRA, Université de Paris Sud, GIS Génoblé

  • Liu Y, Tsunewaki K (1991) Restriction fragment length polymorphism (RFLP) in wheat II. Linkage maps of the RFLP sites in common wheat. Jpn J Genet 66:617–633

    CAS  PubMed  Google Scholar 

  • Liu YG, Mori N, Tsunewaki K (1990) Restriction fragment length polymorphism (RFLP) analysis in wheat I. Genomic DNA library construction and RFLP analysis in common wheat. Jpn J Genet 65:367–380

    CAS  PubMed  Google Scholar 

  • Lu H, Romero-Severson J, Bernardo R (2002) Chromosomal regions associated with segregation distortion in maize. Theor Appl Genet 105:622–628

    Article  Google Scholar 

  • Maeshima M (2000) Vacuolar H+-pyrophosphatase. Biochim Biophys Acta-Biomembr 1465:37–51

    Google Scholar 

  • Messmer MM, Keller M, Zanetti S, Keller B (1999) Genetic linkage map of a wheat × spelt cross. Theor Appl Genet 98:1163–1170

    CAS  Google Scholar 

  • Mingeot D, Jacquemin JM (1999) Mapping of RFLP probes characterized for their polymorphism on wheat. Theor Appl Genet 98:1132–1137

    CAS  Google Scholar 

  • Pestsova E, Ganal MW, Röder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43:689–697

    Article  CAS  PubMed  Google Scholar 

  • Plaschke J, Ganal MW, Röder MS (1995) Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor Appl Genet 91:1001–1007

    CAS  Google Scholar 

  • Rebmann G, Hertig C, Bull J, Mauch F, Dudler R (1991a) Cloning and sequencing of cDNAs encoding a pathogen-induced putative peroxidase of wheat (Triticum aestivum L). Plant Mol Biol 16:329–331

    CAS  PubMed  Google Scholar 

  • Rebmann G, Mauch F, Dudler R (1991b) Sequence of a wheat cDNA-encoding a pathogen-induced thaumatin- like protein. Plant Mol Biol 17:283–285

    CAS  PubMed  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Romeis T, Ludwig AA, Martin R, Jones JDG (2001) Calcium-dependent protein kinases play an essential role in a plant defence response. EMBO J 20:5556–5567

    CAS  PubMed  Google Scholar 

  • Sagi M, Fluhr R (2001) Superoxide production by plant homologues of the gp91(phox) NADPH oxidase. Modulation of activity by calcium and by tobacco mosaic virus infection. Plant Physiol 126:1281–1290

    CAS  PubMed  Google Scholar 

  • Schnurbusch T, Paillard S, Fossati D, Messmer M, Schachermayr G, Winzeler M, Keller B (2003) Dissecting quantitative and durable Stagonospora glume blotch resistance in Swiss winter wheat. Theor Appl Genet (in press)

  • Sears ER (1966) Chromosome mapping with the aid of telocentrics. In: MacKey J (ed) 2nd Wheat Genet Symp, Lund, pp 370–381

  • Siedler H, Messmer MM, Schachermayr GM, Winzeler H, Winzeler M, Keller B (1994) Genetic diversity in European wheat and spelt breeding material based on RFLP data. Theor Appl Genet 88:994–1003

    Google Scholar 

  • Sourdille P, Cadalen T, Guyomarc'h H, Snape JW, Perretant MR, Charmet G, Boeuf C, Bernard S, Bernard M (2003) An update of the Courtot × Chinese Spring intervarietal molecular marker linkage map for the QTL detection of agronomic traits in wheat. Theor Appl Genet 106:530–538

    CAS  PubMed  Google Scholar 

  • Voorips RE (2002) Mapchart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78

    Article  CAS  PubMed  Google Scholar 

  • Xu Y, Zhu L, Xiao J, Huang N, McCouch SR (1997) Chromosomal regions associated with segregation distortion of molecular markers in F2, backcross, doubled-haploid, and recombinant inbred populations in rice (Oryza sativa L.). Mol Gen Genet 253:535–545

    Article  CAS  PubMed  Google Scholar 

  • Yang TB, Segal G, Abbo S, Feldman M, Fromm H (1996) Characterization of the calmodulin gene family in wheat: structure, chromosomal location, and evolutionary aspects. Mol Gen Genet 252:684–694

    Article  CAS  PubMed  Google Scholar 

  • Young ND, Tanksley SD (1989) Restriction fragment length polymorphism maps and the concept of graphical genotypes. Theor Appl Genet 77:95–101

    Google Scholar 

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Acknowledgements

We thank Dr. M. Gale (John Innes Centre, Norwich, UK), Dr. M. Röder, Dr. A. Graner and Dr. N. Stein (IPK Gatersleben, Germany), C. Wilson (USDA-ARS, Albany Calif., USA), Dr. R. Dudler (University of Zürich, Switzerland), Dr. H. Fromm (University of Leeds, UK), Dr. P. Bailey (John Innes Centre, Norwich, UK), Dr. O. Anderson (Albany Calif., USA) and TraitGenetics Inc. (Gatersleben, Germany) for providing clones, unpublished primer sequences or mapping informations. The authors thank Dr. C. Feuillet (University of Zürich, Switzerland) for critical reading of the manuscript. Dr. L. Moreau (INRA Le Moulon, Gif-sur-Yvette, France) is acknowledged for her advice in using Mapmaker. Funding for this work was provided by the Indo-Swiss Collaboration in Biotechnology and grant 3100-065114 from the Swiss National Science Foundation. All experiments conducted during this study comply with current Swiss laws.

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Authors and Affiliations

  1. Swiss Federal Research Station for Agroecology and Agriculture (FAL) Reckenholzstrasse 191, 8046 Zürich, Switzerland

    S. Paillard, M. Winzeler & G. Schachermayr

  2. Institute of Plant Biology, University of Zürich Zollikerstrasse 107, 8008 Zürich, Switzerland

    T. Schnurbusch, O. Abderhalden & B. Keller

  3. Pharmaceutical Institute, University of Basel Benkenstrasse 254, 4108 Witterswil, Switzerland

    M. Messmer

  4. Institut National de la Recherche Agronomique (INRA) Domaine de Crouelle, 234 rue du Brezet, 63039 Clermont-Ferrand, France

    P. Sourdille

  5. Indo-Swiss Collaboration in Biotechnology (ISCB), Institute of Biotechnology, ETH-Hoenggerberg, 8093 Zürich, Switzerland

    G. Schachermayr

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  1. S. Paillard
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  2. T. Schnurbusch
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  8. G. Schachermayr
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Corresponding author

Correspondence to G. Schachermayr.

Additional information

Communicated by H.C. Becker

S. Paillard and T. Schnurbusch contributed equally to the work

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Paillard, S., Schnurbusch, T., Winzeler, M. et al. An integrative genetic linkage map of winter wheat (Triticum aestivum L.). Theor Appl Genet 107, 1235–1242 (2003). https://doi.org/10.1007/s00122-003-1361-6

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  • DOI: https://doi.org/10.1007/s00122-003-1361-6

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