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Fusarium head blight resistance loci in a stratified population of wheat landraces and varieties

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Abstract

To determine if Chinese and Japanese wheat landraces and varieties have unique sources of Fusarium head blight (FHB) resistance, an association mapping panel of 195 wheat accessions including both commercial varieties and landraces was genotyped with 364 genome-wide simple sequence repeat and sequence-tagged site (STS) markers, and evaluated for type II FHB resistance in three greenhouse experiments using single floret inoculation. Population structure analysis stratified this population into five groups with Chinese landraces in four groups. Thirty-two of 51 Chinese landraces and 24 of 27 Japanese accessions were placed in one group. Association analysis using a mixed model identified 11 markers having significant associations with FHB resistance in at least two experiments. Most of these markers coincided with known quantitative trait loci (QTL) for FHB resistance with one potentially novel QTL associated with Xgdm138-5DS and Xgwm358-5DS. Xbarc19-3AS was significant in all three experiments, and the frequency of favorable alleles was more than 53 %. Chinese landraces and Japanese accessions had more favorable alleles at the majority of reproducible marker loci. Nine QTL combinations were identified according to the number of favorable alleles. Mean FHB severities increased with decreasing numbers of favorable alleles at reproducible loci. The resistance loci characterized here will further diversify the wheat FHB resistance gene pool, and provide breeders with additional sources of resistance for improvement of FHB resistance in wheat.

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References

  • Bai G, Shaner G (2004) Management and resistance in wheat and barley to fusarium head blight. Annu Rev Phytopathol 42:135–161

    Article  PubMed  CAS  Google Scholar 

  • Bai G, Kolb FL, Shaner G, Domier LL (1999) Amplified fragment length polymorphism markers linked to a major quantitative trait locus controlling scab resistance in wheat. Phytopathology 89:343–348

    Article  PubMed  CAS  Google Scholar 

  • Ban T, Suenaga K (2000) Genetic analysis of resistance to Fusarium head blight caused by Fusarium graminearum in Chinese wheat cultivar Sumai 3 and the Japanese cultivar Saikai 165. Euphytica 113:87–99

    Article  Google Scholar 

  • Bernardo AN, Ma HX, Zhang DD, Bai GH (2012) Single nucleotide polymorphism in wheat chromosome region harboring Fhb1 for Fusarium head blight resistance. Mol Breed 29:477–488

    Article  Google Scholar 

  • Bradbury PJ, Zhang Z, Kroon DE, Casstevens TM, Ramdoss Y, Buckler ES (2007) TASSEL: software for association mapping of complex traits in diverse samples. Bioinformatics 23:2633–2635

    Article  PubMed  CAS  Google Scholar 

  • Buerstmayr H, Ban T, Anderson JA (2009) QTL mapping and marker-assisted selection for Fusarium head blight resistance in wheat: a review. Plant Breed 128:1–26

    Article  CAS  Google Scholar 

  • Chen J, Griffey CA, Maroof MAS, Stromberg EL, Biyashev RM, Zhao W, Chappell MR, Pridgen TH, Dong Y, Zeng Z (2006) Validation of two major quantitative trait loci for fusarium head blight resistance in Chinese wheat line W14. Plant Breed 125:99–101

    Article  CAS  Google Scholar 

  • del Blanco IA, Frohberg RC, Stack RW, Berzonsky WA, Kianian SF (2003) Detection of QTL linked to Fusarium head blight resistance in Sumai 3-derived North Dakota bread wheat lines. Theor Appl Genet 106:1027–1031

    PubMed  Google Scholar 

  • Ghavami F, Elias EM, Mamidi S, Ansari O, Sargolzaei M, Adhikari T, Mergoum M, Kianian SF (2011) Mixed model association mapping for Fusarium head blight resistance in tunisian-derived durum wheat populations. G3 (Bethesda) 1:209–218

    Article  CAS  Google Scholar 

  • Haberle J, Holzapfel J, Schweizer G, Hartl L (2009) A major QTL for resistance against Fusarium head blight in European winter wheat. Theor Appl Genet 119:325–332

    Article  PubMed  Google Scholar 

  • Hall D, Tegstrom C, Ingvarsson PK (2010) Using association mapping to dissect the genetic basis of complex traits in plants. Brief Funct Genomics 9:157–165

    Article  PubMed  CAS  Google Scholar 

  • Hao C, Wang L, Ge H, Dong Y, Zhang X (2011) Genetic diversity and linkage disequilibrium in Chinese bread wheat (Triticum aestivum L.) revealed by SSR markers. PLoS One 6(2):e17279. doi:10.1371/journal.pone.0017279

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Hao CY, Wang YQ, Hou J, Feuillet C, Balfourier F, Zhang XY (2012) Association mapping and haplotype analysis of a 3.1-Mb genomic region involved in Fusarium head blight resistance on wheat chromosome 3BS. PLoS One 7(10):e46444

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Jayatilake DV, Bai GH, Dong YH (2011) A novel quantitative trait locus for Fusarium head blight resistance in chromosome 7A of wheat. Theor Appl Genet 122:1189–1198

    Article  PubMed  CAS  Google Scholar 

  • Jia G, Chen PD, Qin GJ, Bai GH, Wang X, Wang SL, Zhou B, Zhang SH, Liu DJ (2005) QTLs for Fusarium head blight response in a wheat DH population of Wangshuibai/Alondra’s’. Euphytica 146:183–191

    Article  CAS  Google Scholar 

  • Kollers S, Rodemann B, Ling J, Korzun V, Ebmeyer E, Argillier O, Hinze M, Plieske J, Kulosa D, Ganal MW, Roder MS (2013) Whole genome association mapping of Fusarium head blight resistance in European winter wheat (Triticum aestivum L.). PLoS One 8(2):e57500

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Li C, Zhu H, Zhang C, Lin F, Xue S, Cao Y, Zhang Z, Zhang L, Ma Z (2008) Mapping QTLs associated with Fusarium-damaged kernels in the Nanda 2419 × Wangshuibai population. Euphytica 163:185–191

    Article  CAS  Google Scholar 

  • Li T, Bai GH, Wu SY, Gu SL (2011) Quantitative trait loci for resistance to fusarium head blight in a Chinese wheat landrace Haiyanzhong. Theor Appl Genet 122:1497–1502

    Article  PubMed  Google Scholar 

  • Li T, Bai GH, Wu SY, Gu SL (2012) Quantitative trait loci for resistance to Fusarium head blight in the Chinese wheat landrace Huangfangzhu. Euphytica 185:93–102

    Article  Google Scholar 

  • Lin F, Kong ZX, Zhu HL, Xue SL, Wu JZ, Tian DG, Wei JB, Zhang CQ, Ma ZQ (2004) Mapping QTL associated with resistance to Fusarium head blight in the Nanda2419 × Wangshuibai population. I. Type II resistance. Theor Appl Genet 109:1504–1511

    Article  PubMed  CAS  Google Scholar 

  • Lin F, Xue SL, Zhang ZZ, Zhang CQ, Kong ZX, Yao GQ, Tian DG, Zhu HL, Li CJ, Cao Y, Wei JB, Luo QY, Ma ZQ (2006) Mapping QTL associated with resistance to Fusarium head blight in the Nanda2419 × Wangshuibai population. II: type I resistance. Theor Appl Genet 112:528–535

    Article  PubMed  CAS  Google Scholar 

  • Liu K, Muse SV (2005) PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128–2129

    Article  PubMed  CAS  Google Scholar 

  • Liu SX, Pumphrey MO, Gill BS, Trick HN, Zhang JX, Dolezel J, Chalhoub B, Anderson JA (2008) Toward positional cloning of Fhb1, a major QTL for Fusarium head blight resistance in wheat. Cereal Res Commun 36:195–201

    Article  CAS  Google Scholar 

  • Liu SY, Hall MD, Griffey CA, McKendry AL (2009) Meta-analysis of QTL associated with Fusarium head blight resistance in wheat. Crop Sci 49:1955–1968

    Article  CAS  Google Scholar 

  • Loffler M, Schon C-C, Miedaner T (2009) Revealing the genetic architecture of FHB resistance in hexaploid wheat (Triticum aestivum L.) by QTL meta-analysis. Mol Breed 23:473–488

    Article  Google Scholar 

  • Ma HX, Zhang KM, Gao L, Bai GH, Chen HG, Cai ZX, Lu WZ (2006) Quantitative trait loci for resistance to fusarium head blight and deoxynivalenol accumulation in Wangshuibai wheat under field conditions. Plant Pathol 55:739–745

    Article  CAS  Google Scholar 

  • Maguire TL, Collins GG, Sedgley M (1994) A modified CTAB DNA extraction procedure for plants belonging to the family proteaceae. Plant Mol Biol Report 12:106–109

    Article  CAS  Google Scholar 

  • Mardi M, Buerstmayr H, Ghareyazie B, Lemmens M, Mohammadi SA, Nolz R, Ruckenbauer P (2005) QTL analysis of resistance to Fusarium head blight in wheat using a ‘Wangshuibai’-derived population. Plant Breed 124:329–333

    Article  Google Scholar 

  • Massman J, Cooper B, Horsley R, Neate S, Dill-Macky R, Chao S, Dong Y, Schwarz P, Muehlbauer GJ, Smith KP (2011) Genome-wide association mapping of Fusarium head blight resistance in contemporary barley breeding germplasm. Mol Breed 27:439–454

    Article  Google Scholar 

  • Miedaner T, Wurschum T, Maurer HP, Korzun V, Ebmeyer E, Reif JC (2011) Association mapping for Fusarium head blight resistance in European soft winter wheat. Mol Breed 28:647–655

    Article  Google Scholar 

  • Navara S, Smith KP (2014) Using near-isogenic barley lines to validate deoxynivalenol (DON) QTL previously identified through association analysis. Theor Appl Genet 127:633–645

    Article  PubMed  CAS  Google Scholar 

  • Reif JC, Zhang P, Dreisigacker S, Warburton ML, van Ginkel M, Hoisington D, Bohn M, Melchinger AE (2005) Wheat genetic diversity trends during domestication and breeding. Theor Appl Genet 110:859–864

    Article  PubMed  CAS  Google Scholar 

  • Trail F (2009) For blighted waves of grain: Fusarium graminearum in the postgenomics era. Plant Physiol 149:103–110

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Wingen LU, Orford S, Goram R, Leverington-Waite M, Bilham L, Patsiou TS, Ambrose M, Dicks J, Griffiths S (2014) Establishing the A. E. Watkins landrace cultivar collection as a resource for systematic gene discovery in bread wheat. Theor Appl Genet 127:1831–1842

    Article  PubMed  PubMed Central  Google Scholar 

  • Yang J, Bai G, Shaner GE (2005) Novel quantitative trait loci (QTL) for Fusarium head blight resistance in wheat cultivar Chokwang. Theor Appl Genet 111:1571–1579

    Article  PubMed  CAS  Google Scholar 

  • Yu J, Pressoir G, Briggs WH, Vroh Bi I, Yamasaki M, Doebley JF, McMullen MD, Gaut BS, Nielsen DM, Holland JB, Kresovich S, Buckler ES (2006a) A unified mixed-model method for association mapping that accounts for multiple levels of relatedness. Nat Genet 38:203–208

    Article  PubMed  CAS  Google Scholar 

  • Yu JB, Bai GH, Cai SB, Ban T (2006b) Marker-assisted characterization of Asian wheat lines for resistance to Fusarium head blight. Theor Appl Genet 113:308–320

    Article  PubMed  CAS  Google Scholar 

  • Yu JB, Bai GH, Cai SB, Dong YH, Ban T (2008a) New Fusarium head blight-resistant sources from Asian wheat germplasm. Crop Sci 48:1090–1097

    Article  Google Scholar 

  • Yu JB, Bai GH, Zhou WC, Dong YH, Kolb FL (2008b) Quantitative trait loci for Fusarium head blight resistance in a recombinant inbred population of Wangshuibai/Wheaton. Phytopathology 98:87–94

    Article  PubMed  Google Scholar 

  • Zeng J, Jiang Y (2013) The causal factors for the epidemics of wheat Fusarium head blight in the year of 2012 in China and the strategies for continuous monitoring and prevention. China Plant Prot 33:38–41 (In Chinese)

    Google Scholar 

  • Zhang X, Zhou MP, Ren LJ, Bai GH, Ma HX, Scholten OE, Guo PG, Lu WZ (2004) Molecular characterization of Fusarium head blight resistance from wheat variety Wangshuibai. Euphytica 139:59–64

    Article  CAS  Google Scholar 

  • Zhang XH, Pan HY, Bai GH (2012) Quantitative trait loci responsible for Fusarium head blight resistance in Chinese landrace Baishanyuehuang. Theor Appl Genet 125:495–502

    Article  PubMed  CAS  Google Scholar 

  • Zhou MP, Hayden MJ, Zhang ZY, Lu WZ, Ma HX (2010) Saturation and mapping of a major Fusarium head blight resistance QTL on chromosome 3BS of Sumai 3 wheat. J Appl Genet 51:19–25

    Article  PubMed  Google Scholar 

  • Zhuang YB, Gala A, Yen Y (2013) Identification of functional genic components of major Fusarium head blight resistance quantitative trait loci in wheat cultivar Sumai 3. Mol Plant-Microbe Interact 26:442–450

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We acknowledge financial support by the National Major Project of Breeding for New Transgenic Organisms (2012ZX08009003-004), NSFC (31171537), the Innovative Research Team of Universities in Jiangsu Province, and PAPD. Our thanks also go to Dr. Robert McIntosh (University of Sydney) for language improvement.

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    Authors and Affiliations

    1. Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops; Key Laboratory of Plant Functional Genomics of Ministry of Education; Wheat Research Center, Yangzhou University, Yangzhou, 225009, Jiangsu, China

      Tao Li, Xiali Zhou, Lei Li & Shiliang Gu

    2. Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA

      Dadong Zhang & Guihua Bai

    3. Zhumadian Academy of Agricultural Sciences, Zhumadian, 466000, Henan, China

      Xiali Zhou

    4. USDA-ARS Hard Winter Wheat Genetics Research Unit, Manhattan, KS, 66506, USA

      Guihua Bai

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    1. Tao Li
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    4. Guihua Bai
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    Correspondence to Tao Li.

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    Dadong Zhang and Xiali Zhou have contributed equally to this work.

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    Li, T., Zhang, D., Zhou, X. et al. Fusarium head blight resistance loci in a stratified population of wheat landraces and varieties. Euphytica 207, 551–561 (2016). https://doi.org/10.1007/s10681-015-1539-4

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