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Mapping QTL associated with resistance to Fusarium head blight in the Nanda2419 × Wangshuibai population. I. Type II resistance

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Abstract

Scab disease caused by Fusarium spp. has been a major concern for both wheat producers and consumers. Deployment of scab-resistant varieties is the major strategy to curb this disease. To identify the scab resistance genes in wheat cv. Wangshuibai, we produced a F6:7 recombinant inbred line (RIL) population by crossing Wangshuibai with the scab-susceptible cultivar Nanda2419. The RILs were evaluated for scab resistance in the field by single floret inoculation in two replicates in 2002 and one replicate in 2003. The number of diseased spikelets (NDS) and the length of diseased rachides (LDR) were investigated to reflect the Type II resistance. Among 654 simple sequence repeat (SSR) markers surveyed, 326 were found to be polymorphic between the parents. A partial molecular map was constructed with these markers that covered over 2,210 cM of the wheat genome. Six chromosome regions showed association with both NDS and LDR in a one-way anova analysis, even though the variation explained by them varied between the two traits. Eight intervals were detected for their association with Type II resistance through interval mapping, five of which were not identified in single-point analysis. The quantitative trait loci (QTL) with large effects were the ones in the interval of Xgwm533-3–Xgwm533-1 on chromosome 3B and in the interval of Xwmc539–Xbarc024 on chromosome 6B, whose alleles favoring resistance originate from Wangshuibai. In addition, a QTL whose resistance allele originated from Nanda2419 was consistently detected in the interval of Xs1021m–Xgwm47-1 on chromosome 2B. These results suggest that Wangshuibai is the major source for Type II resistance in this population. The markers associated with these QTL would facilitate the use of scab-resistant genes of Wangshuibai in scab resistance breeding programs of wheat.

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References

  • Anderson JA, Stack RW, Liu S, Waldron BL, Field AD, Coyne C, Moreno-Sevilla B, Mitchell Fetch J, Song QJ, Cregan PB, Frohberg RC (2001) DNA markers for Fusarium head blight resistance QTL in two wheat populations. Theor Appl Genet 102:1164–1168

    Article  CAS  Google Scholar 

  • Arthur JC (1891) Wheat scab. Ind Agric Exp Stn Bull 36:129

    Google Scholar 

  • Bai GH, Kolb FL, Shaner G, Domier LL (1999) Amplified fragment length polymorphism markers linked to a major quantitative trait locus controlling scab resistance in wheat. Phytopath 89:343–348

    CAS  Google Scholar 

  • Bai GH, Guo PG, Kolb FL (2003) Genetic relationships among head blight resistant cultivars of wheat assessed on the basis of molecular markers. Crop Sci 43:498–507

    CAS  Google Scholar 

  • Ban T, Suenaga K (2000) Genetic analysis of resistance to Fusarium head blight caused by Fusarium graminearum in Chinese wheat cultivar Sumai 3 and the Japanese cultivar Saikai 165. Euphytica 113:87–99

    Article  Google Scholar 

  • Basten CJ, Weir BS, Zeng ZB (1994) zmap—a QTL cartgrapher. In: Smith C, Gavora JS, Benkel B, Chesnais J, Fairfull W, Gibson JP, Kennedy BW, Burnside EB (eds) 5th World Congr Genet Applied Livestock Prod. Comput Strategies Software vol 22, pp 65–66http://statgen.ncsu.edu/qtlcart/cartographer/

  • Buerstmayr H, Lemmens M, Hartl L, Doldi L, Steiner B, Stierschneider M, Ruckenbauer P (2002) Molecular mapping of QTL for Fusarium head blight resistance in spring wheat. I. Resistance to fungal spread (Type II resistance). Theor Appl Genet 104:84–91

    Article  CAS  PubMed  Google Scholar 

  • Buerstmayr H, Steiner B, Hartl L, Griesser M, Angerer N, Lengauer D, Miedaner T, Schneider B, Lemmens M (2003) Molecular mapping of QTL for Fusarium head blight resistance in spring wheat. II. Resistance to fungal penetration and spread. Theor Appl Genet 107:503–508

    Article  CAS  PubMed  Google Scholar 

  • Del Blanco IA, Frohberg RC, Stack RW, Berzonsky WA, Kianian SF (2003) Detection of QTL linked to Fusarium head blight resistance in Sumai 3-derived North Dakota bread wheat lines. Theor Appl Genet 106:1027–1031

    PubMed  Google Scholar 

  • Gao L, Tang F, Li H, Jia J (2003) Analysis of microsatellites in major crops assessed by computational and experimental approaches. Mol Breed 12:245–261

    Article  CAS  Google Scholar 

  • Gervais L, Dedryver F, Morlais JY, Bodusseau V, Negre S, Bilous M, Groos C, Trottet M (2003) Mapping of quantitative trait loci for field resistance to Fusarium head blight in an European winter wheat. Theor Appl Genet 106:961–970

    CAS  PubMed  Google Scholar 

  • Gilbert J, Tekauz A (2000) Review: recent developments in research on Fusarium head blight of wheat in Canada. Can J Plant Pathol 22:1–8

    Google Scholar 

  • Gupta A, Lipps PE, Campbell KG (2000) Finding quantitative trait loci associated with Fusarium head blight of wheat using simple sequence repeat markers. In: Ward RW et al. (eds) Proc 2000 Natl Fusarium Head Blight Forum. Michigan State University, East Lansing, pp38–32

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199

    CAS  PubMed  Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daley MJ, Lincoln SE, Newburg L (1987) mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    CAS  PubMed  Google Scholar 

  • Li BQ, Liu FL, Xu RC, Huang C, Cheng FS, Liu JY, Meng J, Mou JM (2000) Sumai No. 3: its development, genetic characteristics, and applications in wheat breeding for Fusarium head blight. In: Raupp J, Ma Z, Chen P, Liu D (eds) Proc Int Symp Wheat Improve Scab Resistance. KSU Printing Services, Manhattan, Kan., pp 187–193

  • Liu SX, Anderson JA (2003) Marker assisted evaluation of Fusarium head blight resistant wheat germplasm. Crop Sci 43:760–766

    CAS  Google Scholar 

  • Liu ZZ, Wang ZY (1991) Improved scab resistance in China: sources of resistance and problems. In: Saunders DA (ed) Wheat for the nontraditional warm areas. Proc Int Conf. CIMMYT, Mexico, DF, pp 178–188

  • Ma ZQ, Sorrells ME (1995) Genetic analysis of fertility restoration in wheat using restriction fragment length polymorphisms. Crop Sci 35:1137–1143

    CAS  Google Scholar 

  • McMullen M, Jones R, Gallenberg D (1997) Scab of wheat and barley: a re-emerging disease of devastating impact. Plant Dis 81:1340–1348

    Google Scholar 

  • Mesterhazy A (1995) Types and components of resistance to Fusarium head blight of wheat. Plant Breed 114:377–386

    Google Scholar 

  • Miller JD, Young JC, Sampson DR (1985) Deoxynivalenol and Fusarium head blight resistance in spring cereals. J Phytopath 113:359–367

    CAS  Google Scholar 

  • Otto CD, Kianian SF, Elias EM, Stack RW, Joppa LR (2002) Genetic dissection of a major Fusarium head blight QTL in tetraploid wheat. Plant Mol Biol 48:625–632

    Google Scholar 

  • Paillard S, Schnurbusch T, Tiwari R, Messmer M, Winzeler M, Keller B, Schachermayr G (2004) QTL analysis of resistance to Fusarium head blight in Swiss winter wheat (Triticum aestivum L.). Theor Appl Genet (in press)

    Article  CAS  PubMed  Google Scholar 

  • Parry DW, Jenkinson P, McLeod L (1995) Fusarium ear blight (scab) in small grain cereals-a review. Plant Pathol 44:207–238

    Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Saur L (1991) Sources of resistance to head blight caused by Fusarium culmorum in bread wheat and related species. Agronomie 11:535–541

    Google Scholar 

  • Schroeder HW, Christensen JJ (1963) Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopath 53:831–838

    Google Scholar 

  • Shen X, Ittu M, Ohm H (2003a) Quantitative trait loci conditioning resistance to Fusarium head blight in wheat line F201R. Crop Sci 43:850–857

    CAS  Google Scholar 

  • Shen X, Zhou M, Lu W, Ohm H (2003b) Detection of Fusarium head blight resistance QTL in a wheat population using bulked segregant analysis. Theor Appl Genet 106:1041–1047

    CAS  PubMed  Google Scholar 

  • Snijders CHA (1990) Genetic variation for resistance to Fusarium head blight in bread wheat. Euphytica 50:171–179

    Google Scholar 

  • Steiner B, Lemmens M, Griesser M, Scholz U, Schondelmaier J, Buerstmayr H (2004) Molecular mapping of resistance to Fusarium head blight in the spring wheat cultivar Frontana. Theor Appl Genet 109:215–224

    Article  CAS  PubMed  Google Scholar 

  • Waldron BL, Moreno-Sevilla B, Anderson JA, Stack RW, Frohberg RC (1999) RFLP mapping of QTL for Fusarium head blight resistance in wheat. Crop Sci 39:805–811

    CAS  Google Scholar 

  • Wang YZ (1996) Epidemiology and management of wheat scab in China. In: CIMMYT (ed) Fusarium head scab: global status and future prospects. CIMMYT, El Batan, pp 97–105

    Google Scholar 

  • Zhou WC, Kolb FL, Bai GH, Shaner G, Domier LL (2002) Genetic analysis of scab resistance QTL in wheat with microsatellite and AFLP markers. Genome 45:719–727

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This project was partially supported by the “973” program (G1998010200), NFSC program (30270807), NSFC-outstanding youth fund and “863” program (2002AA224161, 2003AA207100). We are grateful to Prof. Jizeng Jia for his kindness in providing us the wmc primers. We thank all of the laboratory staffs and graduate students in our laboratory who contributed to this work.

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Authors and Affiliations

  1. National Key Lab of Crop Genetics and Germplasm Enhancement, College of Agricultural Sciences, Nanjing Agricultural University, Nanjing Jiangsu, 210095, China

    F. Lin, Z. X. Kong, H. L. Zhu, S. L. Xue, J. Z. Wu, D. G. Tian, J. B. Wei, C. Q. Zhang & Z. Q. Ma

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  1. F. Lin
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  2. Z. X. Kong
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  3. H. L. Zhu
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  4. S. L. Xue
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  5. J. Z. Wu
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  6. D. G. Tian
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  7. J. B. Wei
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  8. C. Q. Zhang
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  9. Z. Q. Ma
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Correspondence to Z. Q. Ma.

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Communicated by F. Salamini

F. Lin and Z.X. Kong have equally contributed to this work.

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Lin, F., Kong, Z.X., Zhu, H.L. et al. Mapping QTL associated with resistance to Fusarium head blight in the Nanda2419 × Wangshuibai population. I. Type II resistance. Theor Appl Genet 109, 1504–1511 (2004). https://doi.org/10.1007/s00122-004-1772-z

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  • DOI: https://doi.org/10.1007/s00122-004-1772-z

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