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Molecular characterization of Fusarium head blight resistance from wheat variety Wangshuibai

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Abstract

Fusarium head blight (FHB) is a destructive disease of wheat worldwide. FHB resistance genes from Sumai 3 and its derivatives such as Ning 7840 have been well characterized through molecular mapping. In this study, resistance genes in Wangshuibai, a Chinese landrace with high and stable FHB resistance, were analyzed through molecular mapping. A population of 104 F2-derived F7 recombinant inbred lines (RILs) was developed from the cross between resistant landrace Wangshuibai and susceptible variety Alondra‘s’. A total of 32 informative amplified fragment length polymorphism (AFLP) primer pairs (EcoRI/MseI) amplified 410 AFLP markers segregating among the RILs. Among them, 250 markers were mapped in 23 linkage groups covering a genetic distance of 2,430 cM. In addition, 90 simple sequence repeat (SSR) markers were integrated into the AFLP map. Fifteen markers associated with three quantitative trait loci (QTL) for FHB resistance (P < 0.01) were located on two chromosomes. One QTL was mapped on 1B and two others were mapped on 3B. One QTL on 3BS showed a major effect and explained up to 23.8% of the phenotypic variation for type II FHB resistance.

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Abbreviations

cM:

centimorgan

LOD:

log likelihood ratio

RILs:

recombinant inbred lines

AFLP:

amplified fragment length polymorphisms

FHB:

Fusarium head blight

SSR:

simple sequence repeat

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Authors and Affiliations

  1. Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, P.R. China

    Xu Zhang, Miaoping Zhou, Lijuan Ren, Hongxiang Ma & Weizhong Lu

  2. USDA/ARS/PSERU, 4008 Throckmorton Hall, Department of Agronomy, Kansas State University, KS, 66506, U.S.A.

    Guihua Bai

  3. BU Genetics and Breeding, Plant Research International, 6700AA, Wageningen, The Netherlands

    Olga E Scholten

  4. Department of Plant and Soil Sciences, Oklahoma State University, OK, 74078, U.S.A.

    Peiguo Guo

Authors
  1. Xu Zhang
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  2. Miaoping Zhou
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  3. Lijuan Ren
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  4. Guihua Bai
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  5. Hongxiang Ma
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  6. Olga E Scholten
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  7. Peiguo Guo
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  8. Weizhong Lu
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Correspondence to Weizhong Lu.

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Zhang, X., Zhou, M., Ren, L. et al. Molecular characterization of Fusarium head blight resistance from wheat variety Wangshuibai. Euphytica 139, 59–64 (2004). https://doi.org/10.1007/s10681-004-2298-9

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  • DOI: https://doi.org/10.1007/s10681-004-2298-9

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