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Novel quantitative trait loci (QTL) for Fusarium head blight resistance in wheat cultivar Chokwang

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Abstract

Fusarium head blight (FHB) is one of the most destructive diseases in wheat. This study was to identify new quantitative trait loci (QTL) for FHB resistance and the molecular markers closely linked to the QTL in wheat cultivar Chokwang. The primers of 612 simple sequence repeats (SSRs) and 12 target-region-amplified polymorphism (TRAP) marker were analyzed between resistant (Chokwang) and susceptible (Clark) parents. One hundred and seventy-two polymorphic markers were used to screen a population of 79 recombinant inbred lines (RILs) derived from the cross of Chokwang and Clark. One major QTL, Qfhb.ksu-5DL1, was identified on chromosome 5DL. The SSR marker Xbarc 239 was mapped in the QTL region, and also physically located to the bin of 5DL1-0.60-0.74 by using Chinese Spring deletion lines. Another QTL Qfhb.ksu-4BL1was linked to SSR Xbarc 1096 and tentatively mapped on 4BL. A QTL on 3BS, Qfhb.ksu-3BS1, was also detected with marginal significance in this population. Different marker alleles for these QTL were detected between Chokwang and Sumai 3 and its derivatives. These results suggested that Chokwang contains new QTL for FHB resistance that are different from those in Sumai 3. Pyramiding resistance QTL from various sources may enhance FHB resistance in wheat cultivars.

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Abbreviations

FHB:

Fusarium head blight

QTL:

Quantitative trait locus

SSR:

Simple sequence repeat

TRAP:

Target-region-amplified polymorphism

RIL:

Recombinant inbred line

IM:

Interval mapping

CIM:

Composite interval mapping

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Acknowledgements

This research was partly supported by the US Wheat and Barley Scab Initiative. All the aneuploid stocks were kindly provided by Wheat Genetic Resource Center, Kansas State University, USA, and DNA of these aneuploid stocks was kindly provided by Dr. Qi from the Center. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. This is contribution No 05-248-J from the Kansas Agricultural Experiment Station, Manhattan, KS, USA.

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Authors and Affiliations

  1. Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA

    Jun Yang

  2. USDA-ARS, Plant Science and Entomology Research Unit, 4008 Throckmorton Hall, Manhattan, KS, 66506, USA

    Guihua Bai

  3. Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN, 47907, USA

    Gregory E. Shaner

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  1. Jun Yang
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  2. Guihua Bai
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  3. Gregory E. Shaner
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Correspondence to Guihua Bai.

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Communicated by B. Keller

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Yang, J., Bai, G. & Shaner, G.E. Novel quantitative trait loci (QTL) for Fusarium head blight resistance in wheat cultivar Chokwang. Theor Appl Genet 111, 1571–1579 (2005). https://doi.org/10.1007/s00122-005-0087-z

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