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Saturation and mapping of a majorFusarium head blight resistance QTL on chromosome 3BS of Sumai 3 wheat

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Abstract

Fusarium head blight (FHB) is a destructive disease in wheat. The major quantitative trait locus (QTL) on 3BS from Sumai 3 and its derivatives has been used as a major source of the resistance to FHB worldwide, but the discrepancy in reported location of the major QTL could block its using in map based cloning and marker assisted selection. In this study, Chinese Spring-Sumai 3 chromosome 3B substitution line was used as resistant parent of the mapping population to reduce the confounded effect of genetic background in Sumai 3. The major QTL region was saturated with the Sequence Tagged Microsatellite (STM) and Sequence Tagged Site (STS) markers. A linkage map of chromosome 3B with 36 markers covering a genetic distance of 112.4 cM was constructed. Twelve new markers were inserted into the chromosome region where the major QTL was located. The average interval distance between markers was 1.5 cM. Multiple QTL Models (MQM) mapping indicated that the major QTL was located in the interval ofXgwm533 — Xsts9-1, and explained 45.6% of phenotypic variation of the resistance to FHB. The SSR (simple sequence repeat) markerXgwm533 and STM markerXstm748tcac are closely linked to the major QTL.

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References

  • Anderson JA, Stack RW, Liu S, Waldron BL, Fjeld AD, Coyne C, et al. 2001. DNA markers forFusarium head blight resistance QTL in two wheat populations. Theor Appl Genet 102: 1164–1168.

    Article  CAS  Google Scholar 

  • Bai GH, Xiao QP, Mei JF, 1989. Studies on the inheritance of scab resistance in six wheat varieties. Acta Agri Shanghai 5: 17–23. (in Chinese with English abstract)

    CAS  Google Scholar 

  • Bai GH, Shaner GE, 1994. Scab of wheat: prospects for control. Plant Dis 78: 760–766.

    Article  Google Scholar 

  • Bai GH, Kolb FL, Shaner GE, Domier LL, 1999. Amplified fragment length polymorphism markers linked to a major quantitative trait locus controlling scab resistance in wheat. Phytopathology 89: 343–348.

    Article  CAS  PubMed  Google Scholar 

  • Bai GH, Shaner GE, Ohm H, 2000. Inheritance of resistance toFusarium graminearum in wheat. Theor Appl Genet 100: 1–8.

    Article  Google Scholar 

  • Bai GH, Shaner GE, 2004. Management and resistance in wheat and barley toFusarium head blight. Ann Rev Phytopath 42: 135–161.

    Article  CAS  Google Scholar 

  • Buerstmayr H, Lemmens M, Hartl L, Doldi L, Steiner B, Stierschneider M, Ruckenbauer P, 2002. Molecular mapping of QTL forFusarium head blight resistance in spring wheat I Resistance to fungal spread (type II resistance). Theor Appl Genet 104: 84–91.

    Article  CAS  PubMed  Google Scholar 

  • Chen X, Faris JD, Hu J, Stack RW, Adhikari T, Elias EM, et al. 2007. Saturation and comparative mapping of a majorFusarium head blight resistance QTL in tetraploid wheat. Mol Breeding 19: 113–124.

    Article  CAS  Google Scholar 

  • Cuthbert PA, Somers DJ, Thomas J, Cloutier C, Brulé-Babel A, 2006. Fine mapping Fhb1, a major gene controllingFusarium head blight resistance in bread wheat (Triticum aestivum L.). Theor Appl Genet 112: 1465–1472.

    Article  CAS  PubMed  Google Scholar 

  • Cuthbert PA, Somers DJ, Brulé-Babel A, 2007. Mapping of Fhb2 on chromosome 6BS: a gene controllingFusarium head blight field resistance in bread wheat (Triticum aestivum L.). Theor Appl Genet 114: 429–437.

    Article  CAS  PubMed  Google Scholar 

  • Hayden MJ, Stephenson P, Logojan AM, Khatkar D, Rogers C, Koebner RMD, et al. 2004. A new approach to extending the wheat marker pool by anchored PCR amplification of compound SSRs. Theor Appl Genet 108: 733–742.

    Article  CAS  PubMed  Google Scholar 

  • Hayden MJ, Stephenson P, Logojan AM, Khatkar D, Rogers C, Elsden J, et al. 2006. Development and genetic mapping of sequence-tagged microsatellites (STMs) in bread wheat (Triticum aestivum L.) Theor Appl Genet 113: 1271–1281.

    Article  CAS  PubMed  Google Scholar 

  • Jansen RC, Stam P, 1994. High resolution of quantitative traits into multiple loci via interval mapping. Genetics 136: 1447–1455.

    CAS  PubMed  Google Scholar 

  • Kosambi DD, 1944. The estimation of map distance from recombination values. Ann Eugen 12: 172–175.

    Google Scholar 

  • Liu S, Anderson JA, 2003. Targeted molecular mapping of a major wheat QTL forFusarium head blight resistance using wheat ESTs and synteny with rice. Genome 46: 817–823.

    Article  CAS  PubMed  Google Scholar 

  • Liu S, Zhang X, Pumphrey MO, Stack RW, Gill BS, Anderson JA, 2006. Complex microcolinearity among wheat, rice, and barley revealed by fine mapping of the genomic region harboring a major QTL for resistance toFusarium head blight in wheat. Funct Integr Genomics 6: 83–89.

    Article  CAS  PubMed  Google Scholar 

  • Qi L, Echalier B, Chao B, Lazo GR, Butler GE, et al. 2004. A chromosome bin map of 16000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat. Genetics 168: 701–712

    Article  CAS  PubMed  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW, 1998. A SSR map of wheat. Genetics 149: 2007–2023.

    PubMed  Google Scholar 

  • Saghai-Maroof MA, Soliman K, Jorgensen RA, Allard RW, 1984. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81: 8014–8018.

    Article  CAS  PubMed  Google Scholar 

  • Shen X, Zhou M, Lu W, Ohm H, 2003. Detection ofFusarium head blight resistance QTL in a wheat population using bulked segregant analysis. Theor Appl Genet 106: 1041–1047.

    CAS  PubMed  Google Scholar 

  • Somers DJ, Fadak G, Savard M, 2003. Molecular mapping of novel genes controllingFusarium head blight resistance and deoxynivalenol accumulation in spring wheat. Genome 46: 555–564.

    Article  CAS  PubMed  Google Scholar 

  • Van Ginkel M, Van der Schaar W, Yang Z, Rajaram S, 1996. Inheritance of resistance to scab in two wheat cultivars from Brazil and China. Plant Disease 80: 863–867.

    Article  Google Scholar 

  • Van Ooijen JW, Voorrips RE, 2001. JoinMap 3.0, Software for the calculation of genetic linkage maps. Plant Research International, Wageningen, The Netherlands.

    Google Scholar 

  • Van Ooijen JW, Boer MP, Jansen RC, Maliepaard C, 2002. MapQTL 4.0, Software for the calculation of QTL positions on genetic maps. Plant Research International, Wageningen, The Netherlands.

    Google Scholar 

  • Waldron BL, Moreno-Sevilla B, Anderson JA, Stack RW, Frohberg RC, 1999. RFLP Mapping of QTL forFusarium head blight resistance in wheat. Crop Sci 39: 805–811.

    Article  CAS  Google Scholar 

  • Yao JB, Ge YF, Wang SW, Yao GC, Zhou CF, Qian CM, 1997. Chromosomal location of genes for scab resistance in wheat cultivar Sumai 3. Scientia Agricultural Sinica 23: 450–453. (in Chinese with English abstract)

    Google Scholar 

  • Zhou MP, Ren LJ, Zhang X, Scholten OE, Shen XR, Bai GH, et al. 2003. Analysis of major QTL forFusarium head blight resistance on the short arm of chromosome 3B in wheat. Acta Genetica Sinica 30: 571–576. (in Chinese with English abstract)

    CAS  PubMed  Google Scholar 

  • Zhou WC, Kolb FL, Bai GH, Shaner GE, Domier LL, 2002a. Genetic analysis of scab resistance QTL in wheat with SSR and AFLP markers. Genome 45: 719–727.

    Article  CAS  PubMed  Google Scholar 

  • Zhou WC, Kolb FL, Bai GH, Domier LL, Boze LK, Smith NJ, 2002b. Effect of individual Sumai 3 chromosomes on resistance to scab spread within spikes and deoxynivalenol accumulation within kernels in wheat. Hereditas 137: 81–89.

    Article  CAS  PubMed  Google Scholar 

  • Zhou WC, Kolb FL, Yu JB, Bai GH, Domier LL, Boze LK, Domier LL, 2004. Molecular characterization ofFusarium head blight resistance in Wangshuibai with simple sequence repeat and amplified fragment length polymorphism markers. Genome 47: 1137–1143.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Zhongling street 50, 210014, Nanjing, P.R.China

    M. P. Zhou, W. Z. Lu & H. -X. Ma

  2. Molecular Plant Breeding CRC, PMB1, Glen Osmond, SA, Australia

    M. J. Hayden

  3. Institute of Crop Science, Chinese Academy of Agricultural Sciences, P.R.China

    Z. Y. Zhang

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  1. M. P. Zhou
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  2. M. J. Hayden
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  3. Z. Y. Zhang
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  4. W. Z. Lu
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  5. H. -X. Ma
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Correspondence to H. -X. Ma.

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Zhou, M.P., Hayden, M.J., Zhang, Z.Y. et al. Saturation and mapping of a majorFusarium head blight resistance QTL on chromosome 3BS of Sumai 3 wheat. J Appl Genet 51, 19–25 (2010). https://doi.org/10.1007/BF03195706

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  • DOI: https://doi.org/10.1007/BF03195706

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