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Investigating successive Australian barley breeding populations for stable resistance to leaf rust

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Genome-wide association studies of barley breeding populations identified candidate minor genes for pairing with the adult plant resistance gene Rph20 to provide stable leaf rust resistance across environments.

Abstract

Stable resistance to barley leaf rust (BLR, caused by Puccinia hordei) was evaluated across environments in barley breeding populations (BPs). To identify genomic regions that can be combined with Rph20 to improve adult plant resistance (APR), two BPs genotyped with the Diversity Arrays Technology genotyping-by-sequencing platform (DArT-seq) were examined for reaction to BLR at both seedling and adult growth stages in Australian environments. An integrated consensus map comprising both first- and second-generation DArT platforms was used to integrate QTL information across two additional BPs, providing a total of four interrelated BPs and 15 phenotypic data sets. This enabled identification of key loci underpinning BLR resistance. The APR gene Rph20 was the only active resistance region consistently detected across BPs. Of the QTL identified, RphQ27 on chromosome 6HL was considered the best candidate for pairing with Rph20. RphQ27 did not align or share proximity with known genes and was detected in three of the four BPs. The combination of RphQ27 and Rph20 was of low frequency in the breeding material; however, strong resistance responses were observed for the lines carrying this pairing. This suggests that the candidate minor gene RphQ27 can interact additively with Rph20 to provide stable resistance to BLR across diverse environments.

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Acknowledgements

This research was supported by the Grains Research and Development Corporation of Australia (UQ00056; US00070). The authors give thanks to Ms Julie McKavanagh (DAF), Ms Janet Barsby (DAF), Mr Ryan Fowler (DAF and QAAFI), and Mr Matthew Williams (PBI, University of Sydney) for the technical assistance in the laboratory and field. We would also like to acknowledge statistical support provided by Miss Bethany Macdonald, Mr Eric Dinglasan, and Dr. Kai Voss-Fels.

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Authors and Affiliations

  1. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD, 4072, Australia

    L. A. Ziems, E. S. Mace, D. R. Jordan & L. T. Hickey

  2. Department of Agronomy and Plant Genetics, University of Minnesota, St Paul, MN, 55108, USA

    J. D. Franckowiak

  3. Department of Agriculture and Fisheries, Hermitage Research Facility, Warwick, QLD, 4370, Australia

    G. J. Platz & E. S. Mace

  4. The University of Sydney, Plant Breeding Institute, Narellan, NSW, 2567, Australia

    R. F. Park & D. Singh

Authors
  1. L. A. Ziems
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  2. J. D. Franckowiak
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  3. G. J. Platz
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  4. E. S. Mace
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  5. R. F. Park
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  6. D. Singh
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  7. D. R. Jordan
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  8. L. T. Hickey
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Contributions

J.D.F., L.T.H., D.R.J., and R.F.P. managed the project; D.R.J., E.S.M., and G.J.P. designed the experiments; J.D.F. provided the plant materials; G.J.P. and D.S. conducted and scored the leaf rust screenings; L.A.Z. and L.T.H. led the data analysis with contributions from E.S.M.; L.A.Z. wrote the manuscript with assistance from all the other authors.

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Correspondence to L. A. Ziems.

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Additional information

Communicated by Dr. Xiaoquan Qi.

Electronic supplementary material

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122_2017_2970_MOESM1_ESM.pptx

Sequence information for markers used for gene designation or peak marker of each QTL. Markers selected were the most strongly associated with leaf rust resistance within each QTL. Marker name and chromosome position are also presented. Supplementary material 1 (PPTX 86 kb)

122_2017_2970_MOESM2_ESM.pptx

Genomic regions for reaction to leaf rust identified from association mapping using four breeding populations from the Northern Region Barley Breeding (NRBB) Program in Australia. BP1 and 2 are depicted in grey and BP3 and 4 in black. Known Rph genes have been positioned on the map along with QTL reported in the previous mapping studies. The key depicts the color coding for the discovery QTL mapping studies. For graphical display purposes, if the QTL confidence interval (CI) was <4 cM or the QTL was based on a single marker only, a CI of 2cM was used. Supplementary material 2 (PPTX 653 kb)

122_2017_2970_MOESM3_ESM.pptx

Mean disease response for lines carrying different combinations of Rph20 and RphQ27 (6H) at 2013 filed sites COB2013_Adult (a) and LRF2013Adult (b). Supplementary material 3 (PPTX 78 kb)

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Ziems, L.A., Franckowiak, J.D., Platz, G.J. et al. Investigating successive Australian barley breeding populations for stable resistance to leaf rust. Theor Appl Genet 130, 2463–2477 (2017). https://doi.org/10.1007/s00122-017-2970-9

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