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Association mapping of resistance to Puccinia hordei in Australian barley breeding germplasm

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“To find stable resistance using association mapping tools, QTL with major and minor effects on leaf rust reactions were identified in barley breeding lines by assessing seedlings and adult plants.”

Abstract

Three hundred and sixty (360) elite barley (Hordeum vulgare L.) breeding lines from the Northern Region Barley Breeding Program in Australia were genotyped with 3,244 polymorphic diversity arrays technology markers and the results used to map quantitative trait loci (QTL) conferring a reaction to leaf rust (Puccinia hordei Otth). The F3:5 (Stage 2) lines were derived or sourced from different geographic origins or hubs of international barley breeding ventures representing two breeding cycles (2009 and 2011 trials) and were evaluated across eight environments for infection type at both seedling and adult plant stages. Association mapping was performed using mean scores for disease reaction, accounting for family effects using the eigenvalues from a matrix of genotype correlations. In this study, 15 QTL were detected; 5 QTL co-located with catalogued leaf rust resistance genes (Rph1, Rph3/19, Rph8/14/15, Rph20, Rph21), 6 QTL aligned with previously reported genomic regions and 4 QTL (3 on chromosome 1H and 1 on 7H) were novel. The adult plant resistance gene Rph20 was identified across the majority of environments and pathotypes. The QTL detected in this study offer opportunities for breeding for more durable resistance to leaf rust through pyramiding multiple genomic regions via marker-assisted selection.

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Acknowledgments

This research was supported by the Grains Research and Development Corporation of Australia (UQ00056). The authors thank Ms Julie McKavanagh (DAFFQ), Mr Ryan Fowler (DAFFQ and QAAFI) and Ms Janet Barsby (DAFFQ) for technical assistance in the laboratory and field. We also wish to acknowledge the staff at the University of Sydney for providing seedling and adult plant data on leaf rust responses for the breeding populations assessed at Cobbitty.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD, 4072, Australia

    L. A. Ziems & L. T. Hickey

  2. Department of Agriculture, Fisheries and Forestry, Hermitage Research Facility, Warwick, QLD, 4370, Australia

    C. H. Hunt, E. S. Mace, G. J. Platz & J. D. Franckowiak

  3. Queensland Alliance for Agriculture and Food Innovation, Hermitage Research Facility, The University of Queensland, Warwick, QLD, 4370, Australia

    D. R. Jordan

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  1. L. A. Ziems
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  2. L. T. Hickey
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Corresponding author

Correspondence to L. A. Ziems.

Additional information

Communicated by X. Qi.

Electronic supplementary material

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122_2014_2291_MOESM1_ESM.xls

Supplementary material 1 The integrated map used as the reference map for QTL projection including Wenzl et al. (2006), RWA (ND19119-5/PI 642914) and ND24260/Flagship maps, including a total of 3,476 markers positions (XLS 385 kb)

Supplementary material 2 (DOCX 25 kb)

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Ziems, L.A., Hickey, L.T., Hunt, C.H. et al. Association mapping of resistance to Puccinia hordei in Australian barley breeding germplasm. Theor Appl Genet 127, 1199–1212 (2014). https://doi.org/10.1007/s00122-014-2291-1

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  • DOI: https://doi.org/10.1007/s00122-014-2291-1

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