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PCR markers for selection of adult plant leaf rust resistance in barley (Hordeum vulgare L.)

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Abstract

Adult plant resistance (APR) is considered potentially more durable for controlling barley leaf rust than seedling Rph (Resistance to Puccinia hordei) genes. A major gene for adult plant resistance to barley leaf rust has been mapped to the telomere region of chromosome 5HS. PCR-based molecular markers were developed for saturation of this region based on previously mapped simple sequence repeat, restriction fragment length polymorphism and Diversity Arrays Technology markers. In addition, defence gene homologue (DGH) and wheat expressed sequence tags mapped in specific bins were used to develop new PCR markers. Seventeen PCR-based markers were mapped to the short arm of chromosome 5H in 292 doubled haploid lines from a cross of Pompadour × Stirling, in which seven markers were mapped within 5 cM of the APR gene. The closest linked marker was about 0.7 cM from the APR gene. The wheat deletion bin map together with defence gene homologues was demonstrated to be an efficient tool for development of new molecular markers associated with the disease resistance gene. Four DGH markers were associated with the APR gene. The new molecular markers are a useful tool for marker-assisted selection of the APR gene and provided a better understanding of the molecular mechanism for leaf rust resistance.

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Abbreviations

AFLP:

Amplified fragment length polymorphism

APR:

Adult plant resistance

CAPS:

Cleaved amplified polymorphic sequence

DArT:

Diversity Arrays Technology

DH:

Doubled haploid

DGH:

Defence gene homologue

MAP3K:

Mitogen-activated protein kinase

Rph :

Resistance to Puccinia hordei

SSCP:

Single-strand conformation polymorphism

WIR1 :

Wheat induced resistance gene 1

References

  • Aghnoum R, Marcel TC, Johrde A, Pecchioni N, Schweizer P, Niks RE (2010) Basal host resistance of barley to powdery mildew: connecting quantitative trait loci and candidate genes. Mol Plant Microbe Interact 23:91–102. doi:10.1094/MPMI-23-1-0091

    Article  PubMed  CAS  Google Scholar 

  • Ashida T, Nasuda S, Sat K, Endo TR (2007) Dissection of barley chromosome 5H in common wheat. Genes Genet Syst 82:123–133

    Article  PubMed  CAS  Google Scholar 

  • Bansal U, Hayden M, Venkata B, Khanna R, Saini R, Bariana H (2008) Genetic mapping of adult plant leaf rust resistance genes Lr48 and Lr49 in common wheat. Theor Appl Genet 117:307–312

    Article  PubMed  CAS  Google Scholar 

  • Bellgard M, Ye J, Gojobori T, Appels R (2004) The bioinformatics challenges in comparative analysis of cereal genomes—an overview. Funct Integr Genomics 4:1–11

    Article  PubMed  CAS  Google Scholar 

  • Draper J, Mur LAJ, Jenkins G, Ghosh-Biswas GC, Bablak P, Hasterok R, Routledge APM (2001) Brachypodium distachyon. A new model system for functional genomics in grasses. Plant Physiol 127:1539–1555. doi:10.1104/pp.010196

    Article  PubMed  CAS  Google Scholar 

  • Golegaonkar PG, Singh D, Park RF (2009) Evaluation of seedling and adult plant resistance to Puccinia hordei in barley. Euphytica 166:183–197

    Article  CAS  Google Scholar 

  • Hiebert CW, Thomas JB, Somers DJ, McCallum BD, Fox SL (2007) Microsatellite mapping of adult-plant leaf rust resistance gene Lr22a in wheat. Theor Appl Genet 115:877–884

    Article  PubMed  CAS  Google Scholar 

  • Hori K, Kobayashi T, Sato K, Takeda K (2005a) QTL analysis of Fusarium head blight resistance using a high-density linkage map in barley. Theor Appl Genet 111:1661–1672

    Article  PubMed  CAS  Google Scholar 

  • Hori K, Sato K, Nankaku N, Takeda K (2005b) QTL Analysis in recombinant chromosome substitution lines and doubled haploid lines derived from a cross between Hordeum vulgare ssp. vulgare and Hordeum vulgare ssp. spontaneum. Mol Breed 16:295–311

    Article  CAS  Google Scholar 

  • Li C, Ni P, Francki M, Hunter A, Zhang Y, Schibeci D, Li H, Tarr A, Wang J, Cakir M, Yu J, Bellgard M, Lance R, Appels R (2004) Genes controlling seed dormancy and pre-harvest sprouting in a rice-wheat-barley comparison. Funct Integr Genomics 4:84–93

    Article  PubMed  CAS  Google Scholar 

  • Li CD, Gupta S, Zhang XQ, Westcott S, Yang J, Park R, Platz G, Loughman R, Lance R (2010) A major QTL controlling adult plant resistance for barley leaf rust. Plant Breed

  • Mammadov J, Steffenson B, Maroof M (2005) High-resolution mapping of the barley leaf rust resistance gene Rph5 using barley expressed sequence tags (ESTs) and synteny with rice. Theor Appl Genet 111:1651–1660

    Article  PubMed  CAS  Google Scholar 

  • Manly KF (1993) A Macintosh program for storage and analysis of experimental genetic mapping data. Mamm Genome 4:303–313

    Article  PubMed  CAS  Google Scholar 

  • Marcel TC (2007) Genetic architecture of basal resistance of barley to Puccinia hordei. Dissertation, Wageningen University

  • Marcel TC, Aghnoum R, Durand J, Varshney RK, Niks RE (2007a) Dissection of the Barley 2L1.0 Region Carrying the ‘Laevigatum’ quantitative resistance gene to leaf rust using near-isogenic lines (NILs) and sub-NILs. Mol Plant Microbe Interact 20:1604–1615. doi:10.1094/MPMI-20-12-1604

    Article  PubMed  CAS  Google Scholar 

  • Marcel TC, Varshney RK, Barbieri M, Jafary H, de Kock MJD, Graner A, Niks RE (2007b) A high-density consensus map of barley to compare the distribution of QTLs for partial resistance to Puccinia hordei and of defence gene homologues. Theor Appl Genet 114:487–500. doi:10.1007/s00122-006-0448-2

    Article  PubMed  CAS  Google Scholar 

  • Marcel TC, Gorguet B, Ta MT, Kohutova Z, Vels A, Niks RE (2008) Isolate specificity of quantitative trait loci for partial resistance of barley to Puccinia hordei confirmed in mapping populations and near-isogenic lines. New Phytol 177:743–755. doi:10.1111/j.1469-8137.2007.02298.x

    Article  PubMed  Google Scholar 

  • Martins-Lopes P, Zhang H, Koebner R (2001) Detection of single nucleotide mutations in wheat using single strand conformation polymorphism gels. Plant Mol Biol Rep 19:159–162

    Article  CAS  Google Scholar 

  • Park RF (2003) Pathogenic specialization and pathotype distribution of Puccinia hordei in Australia, 1992–2001. Plant Dis 87:1311–1316

    Article  CAS  Google Scholar 

  • Park RF (2008) Breeding cereals for rust resistance in Australia. Plant Pathol 57:591–602

    Article  Google Scholar 

  • Park RF, Karakousis A (2002) Characterization and mapping of gene Rph19 conferring resistance to Puccinia hordei in the cultivar ‘Reka 1’ and several Australian barleys. Plant Breed 121:232–236

    Article  CAS  Google Scholar 

  • Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust severity on leaves and stems of cereals. Can J Res C 26:496–500

    Article  Google Scholar 

  • Qi XQ, Stam P, Lindhout P (1998a) Use of locus-specific AFLP markers to construct a high-density molecular map in barley. Theor Appl Genet 96:376–384

    Article  CAS  Google Scholar 

  • Qi XQ, Niks RE, Stam P, Lindhout P (1998b) Identification of QTLs for partial resistance to leaf rust (Puccinia hordei) in barley. Theor Appl Genet 96:1205–1215

    Article  CAS  Google Scholar 

  • Qi XQ, Jiang G, Chen W, Niks RE, Stam P, Lindhout P (1999) Isolate-specific QTLs for partial resistance to Puccinia hordei in barley. Theor Appl Genet 99:877–884

    Article  CAS  Google Scholar 

  • Qi XQ, Fufa F, Sijtsma D, Niks RE, Lindhout P, Stam P (2000) The evidence for abundance of QTLs for partial resistance to Puccinia hordei on the barley genome. Mol Breed 6:1–9

    Article  CAS  Google Scholar 

  • Rogowsky PM, Guidet FLY, Langridge P, Shepherd KW, Koebner RMD (1991) Isolation and characterization of wheat-rye recombinants involving chromosome arm 1DS of wheat. Theor Appl Genet 82:537–544

    Article  CAS  Google Scholar 

  • Savov A, Angelicheva D, Jordanova A, Eigel A, Kalaydjieva L (1992) High percentage acrylamide gels improve resolution in SSCP analysis. Nucleic Acids Res 20:6741–6742. doi:10.1093/nar/20.24.6741

    Article  PubMed  CAS  Google Scholar 

  • Sun X, Bai G, Carver B (2009) Molecular markers for wheat leaf rust resistance gene Lr41. Mol Breed 23:311–321

    Article  CAS  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882. doi:10.1093/nar/25.24.4876

    Article  PubMed  CAS  Google Scholar 

  • Van Os H, Stam P, Visser R, Van Eck H (2005) RECORD: a novel method for ordering loci on a genetic linkage map. Theor Appl Genet 112:30–40

    Article  PubMed  CAS  Google Scholar 

  • Varshney RK, Hoisington DA, Tyagi AK (2006) Advances in cereal genomics and applications in crop breeding. Trends Biotechnol 24:490–499. doi:10.1016/j.tibtech.2006.08.006

    Article  PubMed  CAS  Google Scholar 

  • Woldeab G, Fininsa C, Singh H, Yuen J, Crossa J (2007) Variation in partial resistance to barley leaf rust (Puccinia hordei) and agronomic characters of Ethiopian landrace lines. Euphytica 158:139–151

    Article  Google Scholar 

  • Zigtkiewicz E, Yotova V, Jarnik M, Korab-Laskowska M, Kidd KK, Modiano D, Scozzari R, Stoneking M, Tishkoff S, Batzer M, Labuda D (1997) Nuclear DNA diversity in worldwide distributed human populations. Gene 205:161–171

    Article  Google Scholar 

Download references

Acknowledgments

The project is supported by the Australia Grain Research and Development Corporation. Fenglou Liu received scholarship from the Chinese Scholarship Council. Jason Bradley, Cathy Burchell, Ruihua Lu and Vera Limadinata provided technical support to this research.

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Authors and Affiliations

  1. College of Agronomy, Northwest A&F University, No. 3 Taicheng Road, 712100, Yangling, Shaanxi, People’s Republic of China

    Fenglou Liu

  2. State Agricultural Biotechnology Center, Murdoch University, Perth, WA, 6150, Australia

    Fenglou Liu, Sanjiv Gupta, Xiao-Qi Zhang, Michael Jones & Chengdao Li

  3. Department of Agriculture and Food, Western Australia, 3 Baron-Hay Court, South Perth, WA, 6151, Australia

    Sanjiv Gupta, Robert Loughman, Reg Lance & Chengdao Li

Authors
  1. Fenglou Liu
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  2. Sanjiv Gupta
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  3. Xiao-Qi Zhang
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  4. Michael Jones
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  5. Robert Loughman
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  6. Reg Lance
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  7. Chengdao Li
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Correspondence to Chengdao Li.

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Liu, F., Gupta, S., Zhang, XQ. et al. PCR markers for selection of adult plant leaf rust resistance in barley (Hordeum vulgare L.). Mol Breeding 28, 657–666 (2011). https://doi.org/10.1007/s11032-010-9517-y

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  • DOI: https://doi.org/10.1007/s11032-010-9517-y

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