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Somaclonal Variation and Methods Used for Its Detection

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Propagation and Genetic Manipulation of Plants

Abstract

During plant tissue culture, the genetic stability of in vitro plantlets is often compromised and the underlying factors include the plant species or varieties involved, mode of regeneration used, genotype and ploidy level, composition of the growth medium, duration of the callus phase, and total time in culture. During tissue culture, conversion of the explant into in vitro tissue in fact imposes stress to the plant cells, which undergo a genomic shock and require restructuring of their genomes. An array of morphological, biochemical, and molecular methods has been used to assess somaclonal variation in different plant species and variable efficiency has been observed. The array of methods including molecular markers used for ensuring genetic stability of regenerated cultures in different plants is reviewed in this chapter.

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Authors and Affiliations

  1. Faculty of Agriculture, University of Mauritius, Réduit, Moka, Mauritius

    V. M. Ranghoo-Sanmukhiya

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  1. V. M. Ranghoo-Sanmukhiya
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Correspondence to V. M. Ranghoo-Sanmukhiya .

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  1. Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Iram Siddique

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Ranghoo-Sanmukhiya, V.M. (2021). Somaclonal Variation and Methods Used for Its Detection. In: Siddique, I. (eds) Propagation and Genetic Manipulation of Plants. Springer, Singapore. https://doi.org/10.1007/978-981-15-7736-9_1

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