Summary
Tissue culture of the Zea mays inbred line A188 resulted in the regeneration of plants having a high level of phenotypic variation compared to seed-grown control plants. To determine how such variation was induced and whether this could be related to specific in vitro culture methods, callus cultures were established and maintained on different, commonly used culture media. Plants were regenerated and the genomic DNA of callus cultures and regenerants analysed for RFLP differences. The results show that regardless of the gene probe used, callus formation resulted in significant deviations from the DNA pattern normally found in seed-grown control plants. Alterations in gene copy number also occurred. As differentiation and organogenesis began, the level of DNA variation fell, and most of the regenerated plants showed a genetic similarity to the controls; those with RFLP differences were the somaclonal variants.
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References
Brown PTH (1989) DNA methylation in plants and its role in tissue culture. Genome 31: 717–729
Caboche M, Lark KG (1981) Preferential replication of repeated DNA sequences in nuclei isolated from soybean cells grown in suspension culture. Proc Natl Acad Sci 78: 1731–1735
D'Amato F (1985) Cytogenetics of plant cell and tissue cultures and their regenerants. Crit Rev Plant Sci 3: 73–112
Dhar MS, Dabak MM, Gupta VS, Ranjekar PK (1988) Organisation and properties of repeated DNA sequences in rice genome. Plant Sci 55: 43–52
Edallo S, Zucchinali C, Perenzin M, Salamini F (1981) Chromosomal variation and frequency of spontaneous mutation associated with in vitro culture and plant regeneration in maize. Maydica 26: 39–56
Flavell RB (1985) Repeated sequences and genome change. In: Hohn B, Dennis ES (eds) Genetic flux in plants. Springer, Berlin Heidelberg New York, pp 139–153
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158
Green CE, Phillips RE (1975) Plant regeneration from tissue cultures of maize. Crop Sci 15: 417–421
Hake S, Walbot V (1980) The genome of Zea mays, its organisation and homology to related grasses. Chromosoma 79: 251–270
Hangyel Tarczy M, Feher F, Deak M (1986) Chromosome variation of somaclones in tetraploid lucerne. Noventermeles 35: 281–287
Hartke S, Lörz H (1989) Somatic embryogenesis and plant regeneration from various indica rice genotypes. J Genet Breed 43: 205–219
Johns MA, Strommer JN, Freeling M (1983) Exceptionally high levels of restriction site polymorphism in DNA near the maize Adh1 gene. Genetics 105: 733–743
Karp A, Bright SWJ (1985) On the origins of somaclonal variation. In: Miflin BJ (eds) Oxford surveys of plant and cell biology, vol 2. Oxford University Press. Oxford, pp 199–234
Lapitan NEV, Sears RG, Gill BS (1988) Amplification of repeated sequences in wheat x rye hybrids regenerated from tissue culture. Theor Appl Genet 75: 381–388
Lührs R, Lörz H (1987) Plant regeneration in vitro from embryogenic cultures of spring and winter-type barley (Hordeum vulgare L.) varieties. Theor Appl Genet 75: 16–25
Morrish F, Vasil V, Vasil IK (1987) Developmental morphogenesis and genetic manipulation in tissue cultures of the Gramineae. Adv Genet 24: 431–499
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Müller E, Brown PTH, Hartke S, Lörz H (1990) DNA variation in tissue culture-derived rice plants. Theor Appl Genet 80: 673–679
Peschke VM, Phillips RL, Gengenbach BG (1987) Discovery of transposable element activity among progeny of tissue culture-derived maize plants. Science 238: 804–807
Potrykus I, Harms CT, Lörz H (1979) Callus formation from cell cultures protoplasts of corn Zea mays (L.). Theor Appl Genet 54: 209–214
Rigby PWJ, Diekmann M, Rhodes C, Berg P (1977) Labelling deoxynucleic acid to a high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113: 237–251
Rivin CJ, Cullis CA, Walbot V (1986) Evaluating quantitative variation in the genome of Zea mays. Genetics 113: 1001–1019
Southern EM (1975) Detection of sepcific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517
Swedlund B, Vasil IK (1985) Cytogenetic characterisation of embryogenic callus and regenerated plants of Pennisetum americanum (L.). K. Schum. Theor Appl Genet 69: 575–581
Taylor B, Powell A (1985) Isolation of plant DNA and RNA. Focus 4: 4–6
Zheng KL, Castiglione S, Biasini MG, Biroli A, Morandi C, Scala F (1987) Nuclear DNA amplification in cultured cells of Oryza sativa (L.). Theor Appl Genet 74: 65–70
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Brown, P.T.H., Göbel, E. & Lörz, H. RFLP analysis of Zea mays callus cultures and their regenerated plants. Theoret. Appl. Genetics 81, 227–232 (1991). https://doi.org/10.1007/BF00215727
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DOI: https://doi.org/10.1007/BF00215727