Abstract
Transgenic sorghum has trailed behind other cereals in progress due to tissue culture limitations, lack of model genotypes, low regeneration, and lack of sustainability of regeneration through sub-cultures. Particle bombardment and Agrobacterium-mediated methods are frequently preferred methods for production of transgenic sorghum. Immature embryos and shoot apical meristems are the most suited as target material for genetic transformation. Transformation efficiency is improved through tailored in vitro protocols in desirable genotypes. Many agronomically important traits were introduced in sorghum genotypes to improve quality of grain and forage and to increase resistance to biotic and abiotic stresses. Despite several improvements in transgenic technology and its application for sorghum crop improvement, so far there are no reports on the release and cultivation of transgenic sorghum. Deployment of innovative genetic modification technologies that can keep away from GMO classification and biosafety concerns in sorghum can benefit the producers and consumers of sorghum.
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References
Able JA, Rathus C, Godwin ID (2001) The investigation of optimal bombardment parameters for transient and stable transgene expression in sorghum. In Vitro Cell Dev Biol Plant 37:341–348
Amali P, Kingsley SJ, Ignacimuthu S (2014) Enhanced plant regeneration involving somatic embryogenesis from shoot tip explants of Sorghum bicolor (L. Moench). Asian J Plant Sci Res 4(3):26–34
Balakrishna D, Vinod R, Vijaya I, Padmaja PG, Venkatesh Bhat B, Patil JV (2013) Agrobacterium-mediated genetic transformation of sorghum [Sorghum bicolor (L.) Moench] using synthetic CRY1B gene. In: Proceedings of the global consultation on Millets promotion for health and nutritional security, Directorate of Sorghum Research, Hyderabad, 18–20 December 2013, pp 284
Basu A, Maiti MK, Kar S, Sen SK, Pandey B (2007) Transgenic sweet sorghum with altered lignin composition and process of preparation thereof. Nagarjuna Private Limited, Hyderabad, India. Patent no. WO/2008/102241
Battraw M, Hall TC (1991) Stable transformation of Sorghum bicolor protoplasts with chimeric neomycin phosphotransferase II and b-glucuronidase genes. Theor Appl Genet 82:161–168
Bhaskaran S, Smith RH, Paliwal S, Schertz KF (1987) Somaclonal variation from Sorghum bicolor (L.) Moench. cell culture. Plant Cell Tiss Org Cult 9:189–196
Brandão RL, Carneiro NP, de Oliveira AC, Coelho GTCP, Carneiro AA (2012) Genetic transformation of immature sorghum inflorescence via microprojectile bombardment. In: Yelda Ozden Çiftçi (ed) Transgenic plants – advances and limitations, PhD., In Tech, China
Cai T, Butler L (1990) Plant regeneration from embryogenic callus initiated from immature inflorescences of several high-tannin sorghums. Plant Cell Tiss Org Cult 20:101–110
Cai T, Daly B, Butler L (1987) Callus induction and plant regeneration from shoot portions of mature embryos of high tannin sorghum. Plant Cell Tiss Org Cult 9:245–252
Carvalho CHS, Zehr UB, Gunaratna N, Anderson J, Kononowicz HH, Hodges TK, Axtell JD (2004) Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency. Genet Mol Biol 27:259–269
Casas AM, Kononowicz AK, Zehr UB, Tomes DT, Axtell JD, Butler LG, Bressan RA, Hasegawa PM (1993) Transgenic sorghum plants via microprojectile bombardment. Proc Natl Acad Sci U S A 90:11212–11216
Casas AM, Kononowicz AK, Haan TG, Zhang L, Tomes DT, Bressan RA, Hasegawa PM (1997) Transgenic sorghum plants obtained after microprojectile bombardment of immature inflorescences. In Vitro Cell Dev Biol Plant 33:92–100
Cheng M, Hu T, Layton J, Liu CN, Fry JE (2003) Desiccation of plant tissues post- Agrobacterium infection enhances T-DNA delivery and increases stable transformation efficiency in wheat. In Vitro Cell Dev Biol Plant 39:595–604
Devi P, Zhong H, Sticklen M (2004) Production of Transgenic sorghum plants with related HVA1 gene. In: Seetharama N, Godwin ID (eds) Sorghum tissue culture and transformation. Oxford Publishers, New Delhi, pp 75–79
Elkonin LA, Pakhomova NV (2000) Influence of nitrogen and phosphorus on induction embryogenic callus of sorghum. Plant Cell Tiss Org Cult 61:115–123
Elkonin LA, Ravin NV, Leshko EV, Volokhina IV, Chumakov MI, Skryabin AG (2009) In planta agrobacterial transformation of sorghum plants. Biotekhnologiya 1:23–30
Emani C, Sunilkumar G, Rathore KS (2002) Transgene silencing and reactivation in sorghum. Plant Sci 162:181–192
Gao Z, Jayaraj J, Muthukrishnan S, Claflin L, Liang GH (2005a) Efficient genetic transformation of sorghum using a visual screening marker. Genome 48:321–333
Gao Z, Xie X, Ling Y, Muthukrishnan S, Liang GH (2005b) Agrobacterium tumefaciens mediated sorghum transformation using a mannose selection system. Plant Biotechnol J 3:591–599
Gendy C, Sene M, Van Le B, Vidal J, Van Tran TK (1996) Somatic embryogenesis and plant regeneration in Sorghum bicolor (L.) Moench. Plant Cell Rep 15:900–904
Girijashankar V, Swathisree V (2009) Genetic transformation of Sorghum bicolor. Physiol Mol Biol Plant 15(4):287–302
Girijashankar V, Sharma HC, Sharma KK et al (2005) Development of transgenic sorghum for insect resistance against the spotted stem borer (Chilo partellus). Plant Cell Rep 24(9):513–522
Grootboom AW, O’Kennedy MM, Mkhonza NL, Kunert K, Chakauya E, Chikwamba RK (2008) In vitro culture and plant regeneration of sorghum genotypes using immature zygotic embryos as explant source. Int J Bot 4:450–455
Grootboom AW, Mkhonza NL, O’Kennedy MM, Chakauya E, Kunert K, Chikwamba RK (2010) Biolistic mediated sorghum (Sorghum bicolor L. Moench) transformation via Mannose and Bialaphos based selection systems. Int J Bot 6(2):89–94
Gupta S, Khanna VK, Singh R, Garg GK (2006) Strategies for overcoming genotypic limitations of in vitro regeneration and determination of genetic components of variability of plant regeneration traits in sorghum. Plant Cell Tiss Org Cult 86:379–388
Gurel S, Gurel E, Kaur R, Wong J, Meng L, Tan HQ, Lemaux PG (2009) Efficient reproducible Agrobacterium-mediated transformation of sorghum using heat treatment of immature embryos. Plant Cell Rep 28(3):429–444
Hagio T (2002) Adventitious shoot regeneration from immature embryos of sorghum. Plant Cell Tiss Org Cult 68:65–72
Hagio T, Blowers AD, Earle ED (1991) Stable transformation of sorghum cell cultures after bombardment with DNA coated microprojectiles. Plant Cell Rep 10(5):260–264
Harshavardhan D, Rani TS, Ugalanathan K, Seetharama N (2002) An improved protocol for regeneration of Sorghum bicolor from isolated shoot apices. Plant Biotechnol 19(3):163–171
Hill-Ambroz KL, Weeks JT (2001) Comparison of constitutive promoters for sorghum transformation. Cereal Res Commun 29:17–24
Howe A, Sato S, Dweikat I, Fromm M, Clemente T (2006) Rapid and reproducible Agrobacterium-mediated transformation of sorghum. Plant Cell Rep 25(8):784–791
Ignacimuthu S, Premkumar A (2014) Development of transgenic Sorghum bicolor (L.) Moench resistant to the Chilo partellus (Swinhoe) through Agrobacterium-mediated transformation. Mol Biol Genet Eng. doi:http://dx.doi.org/10.7243/2053-5767-2-1
Indukuri V (2014) Introgression of cry1B gene into sweet sorghum (SSV 84) by marker-assisted backcross breeding. Department of Botany, Andhra University, Waltair
Ishida Y, Saito H, Ohta S, Hiei Y, Komari T, Kumashiro T (1996) High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens. Nat Biotechnol 14(6):745–750
Jeoung JM, Krishnaveni S, Muthukrishnan S, Trick HN, Liang GH (2002) Optimization of sorghum transformation parameters using genes for green fluorescent protein and beta–glucuronidase as visual markers. Hereditas 137(1):20–28
Jogeswar G, Ranadheer D, Anjaiah V, Kavi Kishor PB (2007) High frequency somatic embryogenesis and regeneration in different genotypes of Sorghum bicolor (L.) Moench from immature inflorescence explants. In Vitro Cell Dev Biol Plant 43:159–166
Kaeppler HF, Pederson JF (1996) Media effects on phenotype of callus cultures initiated from photoperiod-insensitive, elite inbred sorghum lines. Maydica 41(2):83–89
Kaeppler HF, Pederson JF (1997) Evaluation of 41 elite and exotic inbred sorghum genotypes for high quality callus production. Plant Cell Tissue Organ Cult 48:71–75
Kononowicz AK, Casas AM, Tomes DT, Bressan RA, Hasegawa PM (1995) New vistas are opened for sorghum improvement by genetic transformation. Afr Crop Sci J 3(2):171–180
Kosambo-Ayoo LM, Bader M, Loerz H, Becker D (2011) Transgenic sorghum (Sorghum bicolor L. Moench) developed by transformation with chitinase and chitosanase genes from Trichoderma harzianum expresses tolerance to anthracnose. Afr J Biotechnol 10:3659–3670
Krishnaveni S, Jeoung JM, Muthukrishnan S, Liang GH (2001) Transgenic sorghum plants constitutively expressing a rice chitinase gene show improved resistance to stalk rot. J Genet Breed 55:151–158
Kumar V, Campbell LM, Rathore KS (2011) Rapid recovery and characterization of transformants following Agrobacterium-mediated T-DNA transfer to sorghum. Plant Cell Tiss Org Cult 104:137–146
Liu G, Godwin ID (2012) Highly efficient sorghum transformation. Plant Cell Rep 31(6):999–1007
Lu L, Wu X, Yin X, Morrand J, Chen X, Folk WR, Zhang ZJ (2009) Development of marker-free transgenic sorghum [Sorghum bicolor (L.) Moench] using standard binary vectors with bar as a selectable marker. Plant Cell Tiss Org Cult 99:97–108
Maheswari M, Varalaxmi Y, Vijayalakshmi A, Yadav SK, Sharmila P, Venkateswarlu B, Vanaja M, Saradhi PP (2010) Metabolic engineering using mtlD gene enhances tolerance to water deficit and salinity in sorghum. Biol Plant 54(4):647–652
Mall TK (2010) Evaluation of novel input output traits in sorghum through biotechnology. Dissertation, Agronomy, University of Nebraska-Lincoln, Lincoln, Nebraska
Murty UR, Visarada AA, Bharathi M (1990a) Developing tissue culture system for sorghum, Sorghum bicolor (L.) Moench. embryogenic callus induction from elite genotypes. Cereal Res Commun 18(3):257–262
Murty UR, Visarada AA, Bharathi M (1990b) Developing tissue culture system for sorghum, Sorghum bicolor (L.) Moench. plant regeneration from embryogenic callus. Cereal Res Commun 18(4):355–358
Mythili PK, Seetharama N, Reddy VD (1999) Plant regeneration from embryogenic cell suspension cultures of wild sorghum (Sorghum dimidiatum Stapf.). Plant Cell Rep 18:424–428
Nguyen TV, Thu TT, Claeys M, Angenon G (2007) Agrobacterium-mediated transformation of sorghum (Sorghum bicolor (L.) Moench) using an improved in vitro regeneration system. Plant Cell Tiss Org Cult 91:155–164
Oldach KH, Morgenstern A, Rother S, Girgi M, O’Kennedy MM, Lorz H (2001) Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and Sorghum bicolor (L.) Moench. Plant Cell Rep 20(5):416–421
Pandey AK, Bhat BV, Balakrishna D, Seetharama N (2010) Genetic transformation of sorghum (Sorghum bicolor (L.) Moench.). Int J Biotech Biochem 6(1):45–53
Pola S (2011) Leaf discs as a source material for plant tissue culture studies of Sorghum bicolor (L.) Moench. Notulae Scientia Biologicae 3(1):70–78
Pola S, Sarada Mani N, Ramana T (2007) Enhanced shoot regeneration in tissue culture studies of Sorghum bicolor. J Agric Technol 3:275–286
Pola S, Sarada Mani N, Ramana T (2008) Plant tissue culture studies in Sorghum bicolor: immature embryo explants as the source material. Int J Plant Prod 2:1–14
Pola S, Sarada Mani N, Ramana T (2009) Long-term maintenance of callus cultures from immature embryo of Sorghum bicolor. World J Agric Sci 5(4):415–421
Prasad Sant RR (2011) Development of a transformation system for sorghum (Sorghum bicolor L.). Ph.D. thesis, Centre for Tropical Crops and Biocommodities, Queensland University of Technology, Brisbane, Australia
Raghuwanshi A, Birch RG (2010) Genetic transformation of sweet sorghum. Plant Cell Rep 29(9):997–1005
Raju RK, Nagaraju K, Annapurna A, Srinivas R, Gawali HS, Rao SV, Visarada KBRS (2007) Simple methods to generate ample explant sources for genetic transformation of sorghum. In: Poster presented at 8th agricultural science congress held during 15–17 Feb. 2007 at Tamil Nadu Agricultural University (TNAU), Coimbatore, India
Rao AM, Padma Sree K, Kavikishore PB (1995) Enhanced plant regeneration in grain and sweet sorghum by asparagine, praline and cefotaxime. Plant Cell Rep 15:72–75
Rathus C, Nguyen T, Able JA, Gray SJ, Godwin ID (2004) Optimizing sorghum transformation technology via somatic embryogenesis. In: Seetharama N, Godwin ID (eds) Sorghum tissue culture, transformation and genetic engineering. ICRISAT and Oxford Publishers, New Delhi
Ratnala V (2013) Genetic transformation of sorghum for stem borer resistance using cry1Aa gene via Agrobacterium-mediated approach. Dissertation, Department of Botany, Andhra University, Waltair, India
Rose JB, Dunwell JM, Sunderland N (1986) Anther culture of Sorghum bicolor. Plant Cell Tiss Org Cult 6:15–32
Sai Kishore N, Aravinda Lakshmi Y, Pashupatinath E, Ramana Kumari B, Balakrishna D, Rao SV, Seetharama N, Visarada KBRS (2004) A simple system to transgenic sorghum using shoot apical meristem. In: Proceedings of the national symposium biohorizon 2004, 12–13 March, 2004, IIT, Delhi, New Delhi, India
Sai Kishore N, Visarada KBRS, Aravinda Lakshmi Y, Pashupatinath E, Rao SV, Seetharama N (2006) In vitro culture methods in sorghum with shoot tip as the explant material. Plant Cell Rep 25(3):174–182
Sai Kishore N, Visarada KBRS, Rao SV, Seetharama N (2011) Progress and prospects for Agrobacterium-mediated genetic transformation in sorghum in comparison to other cereals. Transgenic Plant J 5(1):27–34
Sairam RV, Seetharama N, Devi PS, Verma A, Murthy UR, Potrykus I (1999) Culture and regeneration of mesophyll derived protoplasts of sorghum (Sorghum bicolor (L.) Moench). Plant Cell Rep 18:972–977
Seetharama N, Sairam RV, Rani TS (2000) Regeneration of sorghum from shoot tip cultures and field performance of the progeny. Plant Cell Tiss Org Cult 61:169–173
Smith RH, Bhaskaran S, Schertz K (1983) Sorghum plant regeneration from aluminum selection medium. Plant Cell Rep 2(3):129–132
Sood P, Bhattacharya A, Sood A (2011) Problems and possibilities of monocot transformation. Biol Plant 55:1–15
Syamala D, Devi P (2003) Efficient regeneration of sorghum, Sorghum bicolor (L.) Moench, from shoot-tip explant. Indian J Exp Biol 41(12):1482–1486
Tadesse Y, Jacobs M (2004) Nutritional quality improvement of sorghum through genetic transformation. In: Seetharama N, Godwin ID (eds) Sorghum tissue culture and transformation. Oxford Publishers, New Delhi, pp 81–84
Tadesse Y, Sagi L, Swennen R, Jacobs M (2003) Optimization of transformation conditions and production of transgenic sorghum (Sorghum bicolor) via microprojectile bombardment. Plant Cell Tiss Org Cult 75:1–18
Urriola J, Rathore KS (2014) Temporal and spatial activities of a rice glutelin promoter in transgenic sorghum. Plant Cell Tiss Org Cult 116:227–234
Visarada KBRS, Sai Kishore N, Balakrishna D, Rao SV (2003) Transient gus expression studies in sorghum to develop a simple protocol for Agrobacterium-mediated genetic transformation. J Genet Breed 57:147–154
Visarada KBRS, Rajani G, Prasad GS, Royer M (2013) Development of transgenic sweet sorghum for tolerance to stem borer. Sorghum Times 9:6
Visarada KBRS, Padmaja PG, Sai Kishore N, Pashupatinath E, Royer M, Seetharama N, Patil JV (2014) Production and evaluation of transgenic sorghum for resistance to stem borer. In Vitro Cell Dev Biol Plant 50:176–189
Wang W, Wang J, Yang C, Li Y, Liu L, Xu J (2007) Pollen-mediated transformation of Sorghum bicolor plants. Biotechnol Appl Biochem 48:79–83
Wu E, Lenderts B, Glassman K, Berezowska-Kaniewska M, Christensen H, Asmus T, Zhen S, Chu U, Cho MJ, Zhao ZY (2014) Optimized Agrobacterium-mediated sorghum transformation protocol and molecular data of transgenic sorghum plants. In Vitro Cell Dev Biol Plant 50:9–18
Zhang W, Dewey R, Boss W, Phillippy B, Qu R (2013) Enhanced Agrobacterium-mediated transformation efficiencies in monocot cells is associated with attenuated defense responses. Plant Mol Biol 81:273–286
Zhao ZY, Cai T, Tagliani L et al (2000) Agrobacterium-mediated sorghum transformation. Plant Mol Biol 44(6):789–798
Zhao ZY, Gu W, Cai T, Tagliani L, Hondred D, Bond D, Schroeder S, Rudert M, Pierce D (2001) High throughput genetic transformation mediated by Agrobacterium tumefaciens in maize. Mol Breed 8:323–333
Zhao L, Liu S, Song S (2010) Optimization of callus induction and plant regeneration from germinating seeds of sweet sorghum (Sorghum bicolor Moench). Afr J Biotechnol 9(16):2367–2374
Zhong H, Wang W, Sticklen M (1998) In vitro morphogenesis of Sorghum bicolor (L.) Moench: efficient plant regeneration from shoot apices. J Plant Physiol 153(5–6):719–726
Zhu H, Muthukrishnan S, Krishnaveni S, Wilde G, Jeoung JM, Liang GH (1998) Biolistic transformation of sorghum using a rice chitinase gene. J Genet Breed 52(3):243–252
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Visarada, K.B.R.S., Sai Kishore, N. (2015). Advances in Genetic Transformation. In: Madhusudhana, R., Rajendrakumar, P., Patil, J. (eds) Sorghum Molecular Breeding. Springer, New Delhi. https://doi.org/10.1007/978-81-322-2422-8_9
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