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Identification and evaluation of two diagnostic markers linked to Fusarium wilt resistance (race 4) in banana (Musa spp.)

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Abstract

Fusarium wilt caused by the fungus Fusarium oxysporum f. sp. cubense race 4 (FOC4) results in vascular tissue damage and ultimately death of banana (Musa spp.) plants. Somaclonal variants of in vitro micropropagated banana can hamper success in propagation of genotypes resistant to FOC4. Early identification of FOC4 resistance in micropropagated banana plantlets is difficult, however. In this study, we identified sequence-characterized amplified region (SCAR) markers of banana associated with resistance to FOC4. Using pooled DNA from resistant or susceptible genotypes and 500 arbitrary 10-mer oligonucleotide primers, 24 random amplified polymorphic DNA (RAPD) products were identified. Two of these RAPD markers were successfully converted to SCAR markers, called ScaU1001 (GenBank accession number HQ613949) and ScaS0901 (GenBank accession number HQ613950). ScaS0901 and ScaU1001 could be amplified in FOC4-resistant banana genotypes (“Williams 8818-1” and Goldfinger), but not in five tested banana cultivars susceptible to FOC4. The two SCAR markers were then used to identify a somaclonal variant of the genotype “Williams 8818-1”, which lost resistance to FOC4. Hence, the identified SCAR markers can be applied for a rapid quality control of FOC4-resistant banana plantlets immediately after the in vitro micropropagation stage. Furthermore, ScaU1001 and ScaS0901 will facilitate marker-assisted selection of new banana cultivars resistant to FOC4.

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Abbreviations

FOC:

Fusarium oxysporum f. sp. cubense

FOC4:

Fusarium oxysporum f. sp. cubense race 4

RAPD:

Random amplified polymorphic DNA

SCAR:

Sequence-characterized amplified region

p.i.:

Post inoculation

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Acknowledgments

This research was financed by funds from the National Key Basic Research Special Foundation of China (2005DKA21000-5-05) and supported by the Programs for the National Public Welfare Scientific Research Institution Foundation (NYS200703) and the Hainan Provincial Natural Science Foundation (No. 807039).

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Authors and Affiliations

  1. Laboratory of Plant Genetic & Breeding, Anhui Agricultural University School of Life Science, 130 Changjiang West Road, Hefei, 230036, People’s Republic of China

    Wei Wang

  2. State Key Laboratory of Biocontrol, School of Life Sciences, SunYat-sen (Zhongshan) University, East Campus, Guangzhou, 510006, People’s Republic of China

    Wei Wang, Christian Staehelin & Dawei Xin

  3. Institute of China Southern Subtropical Crop Research, Chinese Academy of Tropical Agricultural Sciences (CATAS), Zhanjiang, 524091, People’s Republic of China

    Yulin Hu, Dequan Sun & Jianghui Xie

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  1. Wei Wang
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  2. Yulin Hu
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  3. Dequan Sun
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  4. Christian Staehelin
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  6. Jianghui Xie
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Correspondence to Jianghui Xie.

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Wang, W., Hu, Y., Sun, D. et al. Identification and evaluation of two diagnostic markers linked to Fusarium wilt resistance (race 4) in banana (Musa spp.). Mol Biol Rep 39, 451–459 (2012). https://doi.org/10.1007/s11033-011-0758-6

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