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Identification and characterization of RAPD and SCAR markers linked to anthracnose resistance gene in sorghum [Sorghum bicolor (L.) Moench]

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An Erratum to this article was published on 17 November 2006

Abstract

Anthracnose, one of the destructive foliar diseases of sorghum growing in warm humid regions, is incited by the fungus Colletotrichum graminicola.The inheritance of anthracnose resistance was studied using the parental cultivars of Sorghum bicolor (L.) Moench, HC 136 (susceptible to anthracnose) and G 73 (anthracnose resistant). The F1 and F2 plants were inoculated with the local isolates of C. graminicola cultures. The F2 plants showed a segregation ratio of 3 (susceptible): 1(resistant) indicating that the locus for resistance to anthracnose in sorghum accession G 73 segregates as a recessive trait in a cross to susceptible cultivar HC 136. RAPD (random amplified polymorphic DNA) marker OPJ 011437 was identified as marker closely linked to anthracnose resistance gene in sorghum by bulked segregant analysis of HC 136 × G73 derived recombinant inbred lines (RILs) of sorghum. A total of 84 random decamer primers were used to screen polymorphism among the parental genotypes. Among these, only 24 primers were polymorphic. On bulked segregant analysis, primer OPJ 01 amplified a 1437 bp fragment only in resistant parent G 73 and resistant bulk. The marker OPJ 011437 was cloned and sequenced. The sequence of RAPD marker OPJ 011437 was used to generate specific markers called sequence characterized amplified regions (SCARs). A pair of SCAR markers SCJ 01-1 and SCJ 01-2 was developed using Mac Vector program. SCAR amplification of resistant and susceptible parents along with their respective bulks and RILs confirmed that SCAR marker SCJ 01 is at the same loci as that of RAPD marker OPJ 011437 and hence, is linked to anthracnose resistance gene. Resistant parent G 73 and resistant bulk amplified single specific band on PCR amplification using SCAR primer pairs. The RAPD marker OPJ 011437 was mapped at a distance of 3.26 cM apart from the locus governing anthracnose resistance on the sorghum genetic map by the segregation analysis of the RILs. Using BLAST program, it was found that the marker showed 100 per cent alignment with the contig{_}3966 located on the longer arm of chromosome 8 of sorghum genome. Therefore, these identified RAPD and SCAR markers can be used in the resistance-breeding program of sorghum anthracnose by marker-assisted selection.

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Abbreviations

bp:

base pair

cM:

centimorgan

PCR:

polymerase chain reaction

RAPD:

random amplified polymorphic DNA

RIL:

recombinant inbred line

SCAR:

sequence characterized amplified region

References

  • Ali, M.E.K. & H.L. Warren, 1992. Anthracnose of sorghum. p. 203–208. In: W.A.J. de Milliano, R.A. Frederiksen and G.D. Bengston (eds.). Sorghum and millets diseases: A second world review. ICRISAT, Patancheru, A.P. 502324, India.

    Google Scholar 

  • Boora, K.S., R.A. Frederiksen & C.W. Magill, 1998. DNA-based markers for a recessive gene conferring anthracnose resistance in sorghum. Crop Sci 38: 1708–1709.

    Article  CAS  Google Scholar 

  • Boora, K.S., R.A. Frederiksen & C.W. Magill, 1999. A molecular marker that segregates with sorghum leaf blight resistance in one cross is maternally inherited in mother. Mol Gen Genet 261: 317–322.

    Article  PubMed  CAS  Google Scholar 

  • Botstein, B., R.L. White, M. Skolnick & R.W. Dairs, 1980. Construction of a genetic linkage map using restriction fragment length polymorphisms. Am J Hum Genet 32: 314–331.

    PubMed  CAS  Google Scholar 

  • Brinboim, H.C. & J. Dolly, 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7: 1513–1523.

    Google Scholar 

  • Burr, B., F.A. Burr, K. H. Thomson, M. C. Albertson & C. W. Stuber, 1988. Gene mapping with recombinant inbreds in maize. Genetics 118: 519–526.

    PubMed  CAS  Google Scholar 

  • Chague, Y., J.C. Mercier, M. Guenard, A. de Courcel & F. Vedel, 1996. Identification and mapping on chromosome 9 of RAPD markers linked to Sw-5 gene in tomato by bulked segregant analysis. Theor Appl Genet 92: 1045–1051.

    Article  CAS  Google Scholar 

  • Dogget, H. 1988. Sorghum. 2nd ed. John Wiley and Sons, Inc., New York.

    Google Scholar 

  • Duncan, R.R., P.J. Bramel-Cox & F.R. Miller, 1991. Contributions of introduced sorghum germplasm to hybrid development in the USA. p. 69–102. In: H.L. Shands and L.E. Wiesner (eds.). Use of plant introductions in cultivar development: Part 1. CSSA spec. Publ. No. 17, CSSA, Madison, WI.

  • Gold, J., D. Harder, F.T. Smith, T. Aung & J. Procunier, 1999. Development of a molecular marker for rust resistance genes Sr39 and Lr35 in wheat breeding lines. plant Biotechnol. (e-journal)

  • Haldane, J. B. S. & C. H. Waddington, 1931. Inbreeding and linkage. Genetics 16: 357–374.

    CAS  PubMed  Google Scholar 

  • Hernandez, P., A. Martin & G. Dorado, 1999. Development of SCARs by direct sequencing of RAPD products: A practical tool for the introgression and marker-assisted selection of wheat. Mol Breed 5: 245–253.

    Article  CAS  Google Scholar 

  • Kennard, W.C., K. Potter, Dijkhuizen, V. Meglie, J. Staub & M. Harvey, 1994. Linkages among RFLP, RAPD, isozymes, disease resistance and morphological markers in narrow and wide crosses of cucumber. Theor Appl Genet 89: 42–48.

    CAS  Google Scholar 

  • Litt, M. & J.A. Luty, 1989. A hypervariable microsatellite revealed by in vitro amplification of a di-nucleotide repeat within the cardiac muscle actin gene. Am J Hum Genet 44: 397–401.

    PubMed  CAS  Google Scholar 

  • Martin, G.B., J.G.K. Williams & S.D. Tanksley, 1991. Rapid identification of markers linked to a Pseudomonas resistance gene in tomato using random primers and near isogenic lines. Proc Natl Acad Sci USA 88: 2236–2340.

    Article  Google Scholar 

  • Michelmore, R.W., Paran, I. & R.V. Kesseli, 1991. Identification of markers linked to disease resistance genes by bulk segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88: 9829–9832.

    Article  Google Scholar 

  • Mohan, M., S. Nair, A. Bhagwat, T.G. Krishna, & M. Yano, 1997. Genome mapping, molecular markers and marker assisted selection in crop plants. Mol Breed 3: 87–103.

    Article  CAS  Google Scholar 

  • Murray, M.G. & W.F. Thompson, 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8: 4321–4325.

    PubMed  CAS  Google Scholar 

  • Ohmori, T., M. Murata & F. Motoyoshi, 1996. Molecular characterization of RAPD and SCAR markers linked to the Tn-1 locus in tomato. Theor Appl Genet 92: 151–156.

    Article  CAS  Google Scholar 

  • Pandey, S., A. Sindhu & K.S. Boora, 2002. RAPD based DNA markers linked to arthracnose disease resistance in Sorghum bicolor. (L.) Moench Indian J Exptl Biol 40: 206–211.

    Google Scholar 

  • Pande, S., R.P. Thakur, R.I. Karunakar, R. Bandyopadhyay, & Reddy, B.V.S. 1994. Development of screening methods and identification of stable resistance to anthracnose in sorghum. Field Crops Res 38: 157–166.

    Article  Google Scholar 

  • Paran, I., R. Kesseli & Michelmore, R. 1991. Identification of restriction fragment length polymorphism and random amplified polymorphic DNA markers linked to downy mildew resistance genes in lettuce, using near isogenic lines. Genome 34: 1021–1027.

    PubMed  CAS  Google Scholar 

  • Pastor-Corrales, M.A. & R.A. Frederiksen, 1980. Sorghum anthracnose. p. 289–294. In: Sorghum diseases, a world review: Proceedings of the international workshop on sorghum diseases, 11–15 Dec. 1978. ICRISAT, Patancheru, A.P., India.

  • Saghai-Maroof, M.A., K.M. Soliman, R.A. Jorgensen & R.W. Allard, 1984. Ribosomal DNA spacer length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natt Acad Sci USA 81: 8014–8018.

    Article  CAS  Google Scholar 

  • Sobir, T., M. Ohmori, & F. Motoyoshi, 2000. Molecular characterization of the SCAR markers tightly linked to the Tm-2 locus of the genes in Lycopersicon. Theor Appl Genet 101: 64–69.

    Article  CAS  Google Scholar 

  • Tar, M., L. Purnhauser, L. Csosz, A. Mesterhazy & G. Gyulai, 2002. Identification of molecular markers for an efficient leaf rust resistance gene (Lr 29) in wheat. In: Proceedings of the 7th Hungarian Congress on plant Physiology, 2002. Acta Biologica Szegediensis 46(3–4): 133–134.

    Google Scholar 

  • Vos, P., R. Hogers, M. Beeker, M. Reijans, T. Vandelee, M. Hornes, A. Frijlers, J. Pot, J. Peleman, M. Kuiper, & M. Zebecau, 1995. AFLP: A new technique for DNA fingerprinting. Nucleic Acid Res 23: 4407–4414.

    PubMed  CAS  Google Scholar 

  • Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski, & S.V. Tingey, 1990. DNA polymorphisms amplified primers are useful as genetic markers. Nucleic Acid Res 18: 6531–6535.

    PubMed  CAS  Google Scholar 

  • Xu, G.W., C.W. Magill, K.F. Schertz & G.E. Hart, 1994. An RFLP linkage map of Sorghum bicolor (L). Moench Theor Appol Genet 89: 139–145.

    CAS  Google Scholar 

  • Zheng, C., R. Chang, L. Qiu, P. Chen, X. Wu & S. Chen, 2003. Identification and characterization of a RAPD/SCAR marker linked to a resistance gene for soybean mosaic virus in soybean. Euphytica 132: 199–210.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology and Molecular Biology, CCS Haryana Agricultural University, Hisar, 125 004, Haryana, India

    Monika Singh, K. Chaudhary & K. S. Boora

  2. Department of Biochemistry, CCS Haryana Agricultural University, Hisar, 125 004, Haryana, India

    H. R. Singal

  3. Department of Plant Pathology and Microbiology, Texas A & M University, College Station, USA

    C. W. Magill

Authors
  1. Monika Singh
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  2. K. Chaudhary
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  3. H. R. Singal
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  4. C. W. Magill
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  5. K. S. Boora
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Correspondence to Monika Singh.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s10681-006-9306-1

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Singh, M., Chaudhary, K., Singal, H.R. et al. Identification and characterization of RAPD and SCAR markers linked to anthracnose resistance gene in sorghum [Sorghum bicolor (L.) Moench]. Euphytica 149, 179–187 (2006). https://doi.org/10.1007/s10681-005-9065-4

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