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Engineering of NADPH regenerators in Escherichia coli for enhanced biotransformation

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Abstract

Efficient regeneration of NADPH is one of the limiting factors that constrain the productivity of biotransformation processes. In order to increase the availability of NADPH for enhanced biotransformation by engineered Escherichia coli, modulation of the pentose phosphate pathway and amplification of the transhydrogenases system have been conventionally attempted as primary solutions. Recently, other approaches for stimulating NADPH regeneration during glycolysis, such as replacement of native glyceradehdye-3-phosphate dehydrogenase (GAPDH) with NADP-dependent GAPDH from Clostridium acetobutylicum and introduction of NADH kinase catalyzing direct phosphorylation of NADH to NADPH from Saccharomyces cerevisiae, were attempted and resulted in remarkable impacts on NADPH-dependent bioprocesses. This review summarizes several metabolic engineering approaches used for improving the NADPH regenerating capacity in engineered E. coli for whole-cell-based bioprocesses and discusses the key features and progress of those attempts.

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Acknowledgments

This research was supported by Advanced Biomass R&D Center (ABC) (2011-0031359) and WCU (World Class University) program (R320110001018530) through the National Research Foundation of Korea (NRF) both funded by the Ministry of Education, Science and Technology. This work was also supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (2011-0003791). M.D.K. was supported by the Ministry of Knowledge Economy (MKE) and the Korea Institute for Advancement of Technology (KIAT) through the Research and Development for Regional Industry.

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Authors and Affiliations

  1. Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul, 151-921, Korea

    Won-Heong Lee & Jin-Ho Seo

  2. Department of Food Science and Human Nutrition and Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA

    Won-Heong Lee & Yong-Su Jin

  3. Department of Food Science and Biotechnology, Kangwon National University, Chuncheon, 200-701, Korea

    Myoung-Dong Kim

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  1. Won-Heong Lee
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Correspondence to Jin-Ho Seo.

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Lee, WH., Kim, MD., Jin, YS. et al. Engineering of NADPH regenerators in Escherichia coli for enhanced biotransformation. Appl Microbiol Biotechnol 97, 2761–2772 (2013). https://doi.org/10.1007/s00253-013-4750-z

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