Abstract
Intracellular NADPH/NADP+ ratio in cells grown on various production media with different carbon and nitrogen sources had a positive correlation with the thymidine production. To improve thymidine production in a previously engineered E. coli strain, NAD+ kinase was overexpressed in it resulting in the NADPH/NADP+ ratio shifting from 0.184 to 0.267. The [NADH + NADP+]/[NAD+ + NADPH] ratio was, however, not significantly altered. In jar fermentation, 740 mg thymidine l−1 was produced in parental strain, while 940 mg l−1 of thymidine was produced in NAD+ kinase-expressing strain.
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Lee, H.C., Kim, J.S., Jang, W. et al. Thymidine production by overexpressing NAD+ kinase in an Escherichia coli recombinant strain. Biotechnol Lett 31, 1929–1936 (2009). https://doi.org/10.1007/s10529-009-0097-z
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DOI: https://doi.org/10.1007/s10529-009-0097-z