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Genetic analysis of in vitro callus and production of multiple shoots in eggplant

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Abstract

Genetic analysis of four in vitro characters (callus initiation, callus productivity, embryogenic callus percentage, and mean number of regenerated shoots per callus) was conducted using a 6 × 6 diallel cross among four cultivars of eggplant (Solanum melongena) and two related species (S. indicum, S. surattense) showing different in vitro responses. Among the 30 hybrid families, the in vitro performance exceeded the midparent in 19, 29, 18, and 22 cases, respectively, for callus initiation, callus productivity, embryogenic callus induction, and number of regenerated shoots. Both additive and dominant effects were significant and additive gene action was predominant for three of four in vitro characters with the exception of callus productivity. Different degrees of dominance were observed in the expression of the four characters. The broad and narrow sense heritabilities were 0.97 and 0.82 for callus initiation frequency, 0.99 and 0.42 for total amount of callus, 0.99 and 0.92 for E-callus productivity, and 0.99 and 0.73 for mean number of shoots, respectively, suggesting that these traits can be easily transferred into economically important cultivars with low tissue culture response or recalcitrance.

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Abbreviations

MS:

Murashige and Skoog (1962) medium

NAA:

α-Naphthaleneacetic acid

BA:

6-Benzyladenine

KN:

Kinetin

E-callus:

Embryogenic callus

MSKB:

MS with each 2.5 mg/l kinetin + BAP

GCA:

General combining ability

SCA:

Specific combining ability

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Acknowledgements

The author thanks Prof. M. Chittibabu, H.O.D, Statistics Dept., Andhra University, Visakhapatnam for his help in statistical analysis. Thanks are also due to the UGC-SAP for providing facilities for this work.

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Authors and Affiliations

  1. MAS Lab, DSR-ICAR, Rajendranagar, Hyderabad, 500030, Andhra Pradesh, India

    D. V. N. Chakravarthi

  2. Department of Botany, Andhra University, Visakhapatnam, 530003, Andhra Pradesh, India

    Y. V. Rao, M. V. S. Rao & V. Manga

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  1. D. V. N. Chakravarthi
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  2. Y. V. Rao
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  3. M. V. S. Rao
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  4. V. Manga
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Correspondence to D. V. N. Chakravarthi.

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Chakravarthi, D.V.N., Rao, Y.V., Rao, M.V.S. et al. Genetic analysis of in vitro callus and production of multiple shoots in eggplant. Plant Cell Tiss Organ Cult 102, 87–97 (2010). https://doi.org/10.1007/s11240-010-9709-5

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  • DOI: https://doi.org/10.1007/s11240-010-9709-5

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