Abstract
Immature inflorescences of ten rye inbred lines (inbred degree S10 and S11) were cultured on solidified MS medium supplemented with 3.0 mg/dm3 of 2,4-D. According to their capability for callus production explants were classified into two groups : responsive (giving weak or intensive callus production) and non responsive (lack of callus formation). After transferring responsive material into hormone-free medium the regeneration of roots or shoots from the intensive growing callus was observed. Consistent differences between lines in the portion of explants with a certain response were found. They were divided into five groups reacting in the same way. Lines with different in-vitro response were crossed in an incomplete diallel. F1, F2 and F3 generations were analyzed and the following conclusions drawn: the ability for plant regeneration from immature inflorescences in rye is determined by numerous loci, has a recessive character, and both callus production and regeneration suppression may be controlled by complementary genes.
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Rakoczy-Trojanowska, M., Malepszy, S. Genetic factors influencing regeneration ability in rye (Secale cereale L.). I. Immature inflorescences. Theoret. Appl. Genetics 86, 406–410 (1993). https://doi.org/10.1007/BF00838554
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DOI: https://doi.org/10.1007/BF00838554